Protein levels measured by imaging in BRAF(V600E/D) melanoma cell lines following treatment with Vemurafenib in combination with chromatin-targeting compounds (immunofluorescence dataset 3 of 4) - Dataset (ID:20276)

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Cell Small Molecule 1 Small Molecule 2 Small Mol 1 Concentration Small Mol 2 Concentration Small Mol Conc Unit Time Point Time Point Unit Replicate Number of Cells Primary Antibody (488) Secondary Antibody (488) Protein Detected (488) Mean Fluorescence Intensity (488) Primary Antibody (647) Secondary Antibody (647) Protein Detected (647) Mean Fluorescence Intensity (647) Plate Name Plate Well
COLO 858 Vemurafenib UNC1215 0.3160 2.6667
uM
 48
hr
 1 2700 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 375.51 NGFR rabbit IgG(H+L) / donkey NGFR 134.85
Colo858-A2-rep1
C8
COLO 858 Vemurafenib JW55 0.3160 2.6667
uM
 48
hr
 1 2243 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 243.79 NGFR rabbit IgG(H+L) / donkey NGFR 100.13
Colo858-A2-rep1
D3
COLO 858 Vemurafenib Entinostat 0.3160 2.6667
uM
 48
hr
 1 2256 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 128.46 NGFR rabbit IgG(H+L) / donkey NGFR 15.79
Colo858-A2-rep1
D4
COLO 858 Vemurafenib Azacitidine 0.3160 2.6667
uM
 48
hr
 1 840 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 198.97 NGFR rabbit IgG(H+L) / donkey NGFR 20.08
Colo858-A2-rep1
E2
COLO 858 Vemurafenib C646 0.3160 2.6667
uM
 48
hr
 1 2689 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 316.57 NGFR rabbit IgG(H+L) / donkey NGFR 154.25
Colo858-A2-rep1
E3
COLO 858 Vemurafenib Resveratrol 0.3160 2.6667
uM
 48
hr
 1 2795 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 318.85 NGFR rabbit IgG(H+L) / donkey NGFR 129.75
Colo858-A2-rep1
E5
COLO 858 Vemurafenib Daminozide 0.3160 2.6667
uM
 48
hr
 1 3426 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 399.44 NGFR rabbit IgG(H+L) / donkey NGFR 145.60
Colo858-A2-rep1
E6
COLO 858 Vemurafenib Decitabine 0.3160 2.6667
uM
 48
hr
 1 1469 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 264.32 NGFR rabbit IgG(H+L) / donkey NGFR 124.92
Colo858-A2-rep1
F2
COLO 858 Vemurafenib Garcinol 0.3160 2.6667
uM
 48
hr
 1 981 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 330.96 NGFR rabbit IgG(H+L) / donkey NGFR 147.82
Colo858-A2-rep1
F3
COLO 858 Vemurafenib BIX-01294 0.3160 2.6667
uM
 48
hr
 1 561 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 189.27 NGFR rabbit IgG(H+L) / donkey NGFR 24.74
Colo858-A2-rep1
F5
COLO 858 Vemurafenib Methylstat 0.3160 2.6667
uM
 48
hr
 1 1783 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 213.42 NGFR rabbit IgG(H+L) / donkey NGFR 68.18
Colo858-A2-rep1
F6
COLO 858 Vemurafenib Anacardic acid 0.3160 2.6667
uM
 48
hr
 1 1253 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 315.28 NGFR rabbit IgG(H+L) / donkey NGFR 120.19
Colo858-A2-rep1
G3
COLO 858 Vemurafenib MC1568 0.3160 2.6667
uM
 48
hr
 1 1318 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 365.04 NGFR rabbit IgG(H+L) / donkey NGFR 114.40
Colo858-A2-rep1
G4
COLO 858 Vemurafenib Tranylcypromine 0.3160 2.6667
uM
 48
hr
 1 1165 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 304.43 NGFR rabbit IgG(H+L) / donkey NGFR 172.86
Colo858-A2-rep1
G6
COLO 858 Vemurafenib Ischemin 0.3160 2.6667
uM
 48
hr
 1 1323 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 307.48 NGFR rabbit IgG(H+L) / donkey NGFR 114.56
Colo858-A2-rep1
G7
COLO 858 Vemurafenib 0.3160
uM
 48
hr
 1 964 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 364.09 NGFR rabbit IgG(H+L) / donkey NGFR 110.85
Colo858-A2-rep1
H2
COLO 858 Vemurafenib 0.3160
uM
 48
hr
 1 950 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 327.98 NGFR rabbit IgG(H+L) / donkey NGFR 130.86
Colo858-A2-rep1
H3
COLO 858 Vemurafenib 0.3160
uM
 48
hr
 1 673 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 276.26 NGFR rabbit IgG(H+L) / donkey NGFR 130.08
Colo858-A2-rep1
H5
COLO 858 Vemurafenib 0.3160
uM
 48
hr
 1 797 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 303.09 NGFR rabbit IgG(H+L) / donkey NGFR 127.30
Colo858-A2-rep1
H6
COLO 858 Vemurafenib 0.3160
uM
 48
hr
 2 1033 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 359.83 NGFR rabbit IgG(H+L) / donkey NGFR 196.89
Colo858-A2-rep2
A2
COLO 858 Vemurafenib 0.3160
uM
 48
hr
 2 908 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 313.42 NGFR rabbit IgG(H+L) / donkey NGFR 130.36
Colo858-A2-rep2
A3
COLO 858 Vemurafenib 0.3160
uM
 48
hr
 2 1117 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 406.39 NGFR rabbit IgG(H+L) / donkey NGFR 145.48
Colo858-A2-rep2
A5
COLO 858 Vemurafenib 0.3160
uM
 48
hr
 2 923 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 349.90 NGFR rabbit IgG(H+L) / donkey NGFR 131.92
Colo858-A2-rep2
A6
COLO 858 Vemurafenib Decitabine 0.3160 2.6667
uM
 48
hr
 2 1525 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 271.76 NGFR rabbit IgG(H+L) / donkey NGFR 135.82
Colo858-A2-rep2
F2
COLO 858 Vemurafenib Epigallocatechin gallate 0.3160 2.6667
uM
 48
hr
 2 2922 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 321.58 NGFR rabbit IgG(H+L) / donkey NGFR 130.77
Colo858-A2-rep2
E8