Protein levels measured by imaging in BRAF(V600E/D) melanoma cell lines following treatment with Vemurafenib in combination with chromatin-targeting compounds (immunofluorescence dataset 3 of 4) - Dataset (ID:20276)

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Cell Small Molecule 1 Small Molecule 2 Small Mol 1 Concentration Small Mol 2 Concentration Small Mol Conc Unit Time Point Time Point Unit Replicate Number of Cells Primary Antibody (488) Secondary Antibody (488) Protein Detected (488) Mean Fluorescence Intensity (488) Primary Antibody (647) Secondary Antibody (647) Protein Detected (647) Mean Fluorescence Intensity (647) Plate Name Plate Well
A-375 Vemurafenib C646 1.0000 2.6667
uM
 48
hr
 2 2465 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 167.50 NGFR rabbit IgG(H+L) / donkey NGFR 173.88
A375-A2-rep2
E3
A-375 Vemurafenib Mocetinostat 1.0000 2.6667
uM
 48
hr
 2 533 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 254.36 NGFR rabbit IgG(H+L) / donkey NGFR 81.09
A375-A2-rep2
E4
A-375 Vemurafenib Resveratrol 1.0000 2.6667
uM
 48
hr
 2 2903 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 213.67 NGFR rabbit IgG(H+L) / donkey NGFR 191.15
A375-A2-rep2
E5
A-375 Vemurafenib Daminozide 1.0000 2.6667
uM
 48
hr
 2 2889 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 184.32 NGFR rabbit IgG(H+L) / donkey NGFR 208.04
A375-A2-rep2
E6
A-375 Vemurafenib I-BET 1.0000 2.6667
uM
 48
hr
 2 2493 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 130.30 NGFR rabbit IgG(H+L) / donkey NGFR 93.88
A375-A2-rep2
E7
A-375 Vemurafenib Epigallocatechin gallate 1.0000 2.6667
uM
 48
hr
 2 2588 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 185.72 NGFR rabbit IgG(H+L) / donkey NGFR 175.22
A375-A2-rep2
E8
A-375 Vemurafenib Decitabine 1.0000 2.6667
uM
 48
hr
 2 2092 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 222.42 NGFR rabbit IgG(H+L) / donkey NGFR 140.13
A375-A2-rep2
F2
A-375 Vemurafenib Garcinol 1.0000 2.6667
uM
 48
hr
 2 2002 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 190.44 NGFR rabbit IgG(H+L) / donkey NGFR 192.31
A375-A2-rep2
F3
A-375 Vemurafenib Pracinostat 1.0000 2.6667
uM
 48
hr
 2 215 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 234.28 NGFR rabbit IgG(H+L) / donkey NGFR 35.01
A375-A2-rep2
F4
A-375 Vemurafenib BIX-01294 1.0000 2.6667
uM
 48
hr
 2 1250 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 292.19 NGFR rabbit IgG(H+L) / donkey NGFR 42.51
A375-A2-rep2
F5
A-375 Vemurafenib Methylstat 1.0000 2.6667
uM
 48
hr
 2 1381 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 137.28 NGFR rabbit IgG(H+L) / donkey NGFR 43.97
A375-A2-rep2
F6
A-375 Vemurafenib I-BET151 1.0000 2.6667
uM
 48
hr
 2 1718 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 145.96 NGFR rabbit IgG(H+L) / donkey NGFR 72.08
A375-A2-rep2
F7
A-375 Vemurafenib CX-5461 1.0000 2.6667
uM
 48
hr
 2 1191 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 130.69 NGFR rabbit IgG(H+L) / donkey NGFR 133.97
A375-A2-rep2
F8
A-375 Vemurafenib RG-108 1.0000 2.6667
uM
 48
hr
 2 1857 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 209.06 NGFR rabbit IgG(H+L) / donkey NGFR 139.68
A375-A2-rep2
G2
A-375 Vemurafenib Anacardic acid 1.0000 2.6667
uM
 48
hr
 2 1845 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 197.52 NGFR rabbit IgG(H+L) / donkey NGFR 193.43
A375-A2-rep2
G3
A-375 Vemurafenib MC1568 1.0000 2.6667
uM
 48
hr
 2 2431 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 484.57 NGFR rabbit IgG(H+L) / donkey NGFR 158.89
A375-A2-rep2
G4
A-375 Vemurafenib UNC0638 1.0000 2.6667
uM
 48
hr
 2 1362 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 190.15 NGFR rabbit IgG(H+L) / donkey NGFR 72.59
A375-A2-rep2
G5
A-375 Vemurafenib Tranylcypromine 1.0000 2.6667
uM
 48
hr
 2 1762 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 188.67 NGFR rabbit IgG(H+L) / donkey NGFR 224.08
A375-A2-rep2
G6
A-375 Vemurafenib Ischemin 1.0000 2.6667
uM
 48
hr
 2 1740 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 196.06 NGFR rabbit IgG(H+L) / donkey NGFR 168.62
A375-A2-rep2
G7
A-375 Vemurafenib 1.0000
uM
 48
hr
 2 1358 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 192.78 NGFR rabbit IgG(H+L) / donkey NGFR 135.20
A375-A2-rep2
H10
A-375 Vemurafenib 1.0000
uM
 48
hr
 2 1289 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 205.41 NGFR rabbit IgG(H+L) / donkey NGFR 165.25
A375-A2-rep2
H11
A-375 Vemurafenib 1.0000
uM
 48
hr
 2 1350 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 187.39 NGFR rabbit IgG(H+L) / donkey NGFR 169.71
A375-A2-rep2
H2
A-375 Vemurafenib 1.0000
uM
 48
hr
 2 1538 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 187.96 NGFR rabbit IgG(H+L) / donkey NGFR 183.79
A375-A2-rep2
H3
A-375 Vemurafenib 1.0000
uM
 48
hr
 2 1451 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 184.45 NGFR rabbit IgG(H+L) / donkey NGFR 151.04
A375-A2-rep2
H4
A-375 Vemurafenib 1.0000
uM
 48
hr
 2 1282 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 207.71 NGFR rabbit IgG(H+L) / donkey NGFR 172.71
A375-A2-rep2
H5