Protein levels measured by imaging in BRAF(V600E/D) melanoma cell lines following treatment with Vemurafenib in combination with chromatin-targeting compounds (immunofluorescence dataset 3 of 4) - Dataset (ID:20276)

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Cell Small Molecule 1 Small Molecule 2 Small Mol 1 Concentration Small Mol 2 Concentration Small Mol Conc Unit Time Point Time Point Unit Replicate Number of Cells Primary Antibody (488) Secondary Antibody (488) Protein Detected (488) Mean Fluorescence Intensity (488) Primary Antibody (647) Secondary Antibody (647) Protein Detected (647) Mean Fluorescence Intensity (647) Plate Name Plate Well
A-375 Vemurafenib Rucaparib 1.0000 2.6667
uM
 48
hr
 1 2038 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 140.07 NGFR rabbit IgG(H+L) / donkey NGFR 172.41
A375-A2-rep1
C3
A-375 Vemurafenib Belinostat 1.0000 2.6667
uM
 48
hr
 1 279 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 395.49 NGFR rabbit IgG(H+L) / donkey NGFR 21.95
A375-A2-rep1
C4
A-375 Vemurafenib Selisistat 1.0000 2.6667
uM
 48
hr
 1 2960 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 191.58 NGFR rabbit IgG(H+L) / donkey NGFR 184.54
A375-A2-rep1
C5
A-375 Vemurafenib GSK-J2 1.0000 2.6667
uM
 48
hr
 1 2997 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 181.28 NGFR rabbit IgG(H+L) / donkey NGFR 199.06
A375-A2-rep1
C6
A-375 Vemurafenib (+)-JQ1 1.0000 2.6667
uM
 48
hr
 1 1957 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 124.74 NGFR rabbit IgG(H+L) / donkey NGFR 79.92
A375-A2-rep1
C7
A-375 Vemurafenib UNC1215 1.0000 2.6667
uM
 48
hr
 1 2539 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 153.45 NGFR rabbit IgG(H+L) / donkey NGFR 217.23
A375-A2-rep1
C8
A-375 Vemurafenib Vorinostat 1.0000 2.6667
uM
 48
hr
 1 1890 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 229.09 NGFR rabbit IgG(H+L) / donkey NGFR 60.09
A375-A2-rep1
D2
A-375 Vemurafenib JW55 1.0000 2.6667
uM
 48
hr
 1 2571 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 135.73 NGFR rabbit IgG(H+L) / donkey NGFR 138.78
A375-A2-rep1
D3
A-375 Vemurafenib Entinostat 1.0000 2.6667
uM
 48
hr
 1 2292 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 451.09 NGFR rabbit IgG(H+L) / donkey NGFR 264.91
A375-A2-rep1
D4
A-375 Vemurafenib AGK2 1.0000 2.6667
uM
 48
hr
 1 678 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 394.51 NGFR rabbit IgG(H+L) / donkey NGFR 132.96
A375-A2-rep1
D5
A-375 Vemurafenib GSK-J4 1.0000 2.6667
uM
 48
hr
 1 1533 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 141.60 NGFR rabbit IgG(H+L) / donkey NGFR 96.94
A375-A2-rep1
D6
A-375 Vemurafenib (-)-JQ1 1.0000 2.6667
uM
 48
hr
 1 3035 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 159.74 NGFR rabbit IgG(H+L) / donkey NGFR 239.66
A375-A2-rep1
D7
A-375 Vemurafenib IOX2 1.0000 2.6667
uM
 48
hr
 1 2950 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 154.83 NGFR rabbit IgG(H+L) / donkey NGFR 183.99
A375-A2-rep1
D8
A-375 Vemurafenib Azacitidine 1.0000 2.6667
uM
 48
hr
 1 1210 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 261.75 NGFR rabbit IgG(H+L) / donkey NGFR 53.65
A375-A2-rep1
E2
A-375 Vemurafenib C646 1.0000 2.6667
uM
 48
hr
 1 2172 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 156.52 NGFR rabbit IgG(H+L) / donkey NGFR 184.53
A375-A2-rep1
E3
A-375 Vemurafenib Mocetinostat 1.0000 2.6667
uM
 48
hr
 1 304 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 299.57 NGFR rabbit IgG(H+L) / donkey NGFR 87.98
A375-A2-rep1
E4
A-375 Vemurafenib Resveratrol 1.0000 2.6667
uM
 48
hr
 1 2505 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 181.88 NGFR rabbit IgG(H+L) / donkey NGFR 186.11
A375-A2-rep1
E5
A-375 Vemurafenib Daminozide 1.0000 2.6667
uM
 48
hr
 1 2676 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 168.16 NGFR rabbit IgG(H+L) / donkey NGFR 205.28
A375-A2-rep1
E6
A-375 Vemurafenib I-BET 1.0000 2.6667
uM
 48
hr
 1 2375 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 130.55 NGFR rabbit IgG(H+L) / donkey NGFR 81.67
A375-A2-rep1
E7
A-375 Vemurafenib Epigallocatechin gallate 1.0000 2.6667
uM
 48
hr
 1 2367 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 176.81 NGFR rabbit IgG(H+L) / donkey NGFR 170.27
A375-A2-rep1
E8
A-375 Vemurafenib Decitabine 1.0000 2.6667
uM
 48
hr
 1 1969 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 193.22 NGFR rabbit IgG(H+L) / donkey NGFR 150.60
A375-A2-rep1
F2
A-375 Vemurafenib Garcinol 1.0000 2.6667
uM
 48
hr
 1 1325 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 146.88 NGFR rabbit IgG(H+L) / donkey NGFR 175.82
A375-A2-rep1
F3
A-375 Vemurafenib Pracinostat 1.0000 2.6667
uM
 48
hr
 1 137 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 388.80 NGFR rabbit IgG(H+L) / donkey NGFR 39.73
A375-A2-rep1
F4
A-375 Vemurafenib BIX-01294 1.0000 2.6667
uM
 48
hr
 1 825 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 295.68 NGFR rabbit IgG(H+L) / donkey NGFR 33.17
A375-A2-rep1
F5
A-375 Vemurafenib Methylstat 1.0000 2.6667
uM
 48
hr
 1 1079 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 150.72 NGFR rabbit IgG(H+L) / donkey NGFR 55.48
A375-A2-rep1
F6