Protein levels measured by imaging in BRAF(V600E/D) melanoma cell lines following treatment with Vemurafenib in combination with chromatin-targeting compounds (immunofluorescence dataset 3 of 4) - Dataset (ID:20276)

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Cell Small Molecule 1 Small Molecule 2 Small Mol 1 Concentration Small Mol 2 Concentration Small Mol Conc Unit Time Point Time Point Unit Replicate Number of Cells Primary Antibody (488) Secondary Antibody (488) Protein Detected (488) Mean Fluorescence Intensity (488) Primary Antibody (647) Secondary Antibody (647) Protein Detected (647) Mean Fluorescence Intensity (647) Plate Name Plate Well
COLO 858 Vemurafenib Tranylcypromine 0.3160 2.6667
uM
 48
hr
 1 1165 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 304.43 NGFR rabbit IgG(H+L) / donkey NGFR 172.86
Colo858-A2-rep1
G6
COLO 858 Vemurafenib Ischemin 0.3160 2.6667
uM
 48
hr
 1 1323 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 307.48 NGFR rabbit IgG(H+L) / donkey NGFR 114.56
Colo858-A2-rep1
G7
COLO 858 Vemurafenib 0.3160
uM
 48
hr
 1 801 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 272.52 NGFR rabbit IgG(H+L) / donkey NGFR 119.13
Colo858-A2-rep1
H11
COLO 858 Vemurafenib 0.3160
uM
 48
hr
 1 964 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 364.09 NGFR rabbit IgG(H+L) / donkey NGFR 110.85
Colo858-A2-rep1
H2
COLO 858 Vemurafenib 0.3160
uM
 48
hr
 1 797 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 303.09 NGFR rabbit IgG(H+L) / donkey NGFR 127.30
Colo858-A2-rep1
H6
COLO 858 Vemurafenib 0.3160
uM
 48
hr
 1 978 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 291.02 NGFR rabbit IgG(H+L) / donkey NGFR 117.92
Colo858-A2-rep1
H7
COLO 858 Vemurafenib 0.3160
uM
 48
hr
 1 977 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 344.61 NGFR rabbit IgG(H+L) / donkey NGFR 118.55
Colo858-A2-rep1
H9
COLO 858 Vemurafenib 0.3160
uM
 48
hr
 2 988 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 341.76 NGFR rabbit IgG(H+L) / donkey NGFR 146.13
Colo858-A2-rep2
A10
COLO 858 Vemurafenib 0.3160
uM
 48
hr
 2 908 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 313.42 NGFR rabbit IgG(H+L) / donkey NGFR 130.36
Colo858-A2-rep2
A3
COLO 858 Vemurafenib 0.3160
uM
 48
hr
 2 811 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 338.76 NGFR rabbit IgG(H+L) / donkey NGFR 129.39
Colo858-A2-rep2
A4
COLO 858 Vemurafenib 0.3160
uM
 48
hr
 2 923 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 349.90 NGFR rabbit IgG(H+L) / donkey NGFR 131.92
Colo858-A2-rep2
A6
COLO 858 Vemurafenib 0.3160
uM
 48
hr
 2 851 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 376.95 NGFR rabbit IgG(H+L) / donkey NGFR 149.87
Colo858-A2-rep2
A7
COLO 858 Vemurafenib 0.3160
uM
 48
hr
 1 1391 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 358.32 NGFR rabbit IgG(H+L) / donkey NGFR 158.87
Colo858-A2-rep1
A10
COLO 858 Vemurafenib 0.3160
uM
 48
hr
 1 1242 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 296.85 NGFR rabbit IgG(H+L) / donkey NGFR 156.51
Colo858-A2-rep1
A11
COLO 858 Vemurafenib 0.3160
uM
 48
hr
 1 1024 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 345.50 NGFR rabbit IgG(H+L) / donkey NGFR 135.78
Colo858-A2-rep1
A3
COLO 858 Vemurafenib 0.3160
uM
 48
hr
 1 1076 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 288.18 NGFR rabbit IgG(H+L) / donkey NGFR 162.29
Colo858-A2-rep1
A4
COLO 858 Vemurafenib 0.3160
uM
 48
hr
 1 1095 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 337.39 NGFR rabbit IgG(H+L) / donkey NGFR 164.33
Colo858-A2-rep1
A7
COLO 858 Vemurafenib 0.3160
uM
 48
hr
 1 897 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 335.21 NGFR rabbit IgG(H+L) / donkey NGFR 126.80
Colo858-A2-rep1
A8
COLO 858 Vemurafenib Olaparib 0.3160 2.6667
uM
 48
hr
 1 1345 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 290.82 NGFR rabbit IgG(H+L) / donkey NGFR 146.21
Colo858-A2-rep1
B2
COLO 858 Vemurafenib Iniparib 0.3160 2.6667
uM
 48
hr
 1 1703 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 310.30 NGFR rabbit IgG(H+L) / donkey NGFR 122.97
Colo858-A2-rep1
B3
COLO 858 Vemurafenib PFI-1 0.3160 2.6667
uM
 48
hr
 1 1383 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 138.34 NGFR rabbit IgG(H+L) / donkey NGFR 30.41
Colo858-A2-rep1
B7
COLO 858 Vemurafenib UNC669 0.3160 2.6667
uM
 48
hr
 1 2097 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 357.07 NGFR rabbit IgG(H+L) / donkey NGFR 157.23
Colo858-A2-rep1
B8
COLO 858 Vemurafenib Rucaparib 0.3160 2.6667
uM
 48
hr
 1 2594 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 255.48 NGFR rabbit IgG(H+L) / donkey NGFR 179.23
Colo858-A2-rep1
C3
COLO 858 Vemurafenib Rocilinostat 0.3160 2.6667
uM
 48
hr
 2 596 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 175.51 NGFR rabbit IgG(H+L) / donkey NGFR 157.64
Colo858-A2-rep2
B5
COLO 858 Vemurafenib CTB 0.3160 2.6667
uM
 48
hr
 2 1525 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 264.79 NGFR rabbit IgG(H+L) / donkey NGFR 156.84
Colo858-A2-rep2
B4