Protein levels measured by imaging in BRAF(V600E/D) melanoma cell lines following treatment with Vemurafenib in combination with chromatin-targeting compounds (immunofluorescence dataset 3 of 4) - Dataset (ID:20276)

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Cell Small Molecule 1 Small Molecule 2 Small Mol 1 Concentration Small Mol 2 Concentration Small Mol Conc Unit Time Point Time Point Unit Replicate Number of Cells Primary Antibody (488) Secondary Antibody (488) Protein Detected (488) Mean Fluorescence Intensity (488) Primary Antibody (647) Secondary Antibody (647) Protein Detected (647) Mean Fluorescence Intensity (647) Plate Name Plate Well
WM-115 Vemurafenib Epigallocatechin gallate 1.0000 0.5333
uM
 48
hr
 1 1363 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 136.88 NGFR rabbit IgG(H+L) / donkey NGFR 1138.47
WM115-B1-rep1
E8
WM-115 Vemurafenib Decitabine 1.0000 0.5333
uM
 48
hr
 1 1312 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 150.52 NGFR rabbit IgG(H+L) / donkey NGFR 949.09
WM115-B1-rep1
F2
WM-115 Vemurafenib Garcinol 1.0000 0.5333
uM
 48
hr
 1 1246 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 139.16 NGFR rabbit IgG(H+L) / donkey NGFR 1098.79
WM115-B1-rep1
F3
WM-115 Vemurafenib Pracinostat 1.0000 0.5333
uM
 48
hr
 1 1333 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 127.90 NGFR rabbit IgG(H+L) / donkey NGFR 666.71
WM115-B1-rep1
F4
WM-115 Vemurafenib BIX-01294 1.0000 0.5333
uM
 48
hr
 1 1331 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 172.39 NGFR rabbit IgG(H+L) / donkey NGFR 974.77
WM115-B1-rep1
F5
WM-115 Vemurafenib Methylstat 1.0000 0.5333
uM
 48
hr
 1 1289 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 122.93 NGFR rabbit IgG(H+L) / donkey NGFR 1114.18
WM115-B1-rep1
F6
WM-115 Vemurafenib I-BET151 1.0000 0.5333
uM
 48
hr
 1 1213 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 125.34 NGFR rabbit IgG(H+L) / donkey NGFR 683.96
WM115-B1-rep1
F7
WM-115 Vemurafenib CX-5461 1.0000 0.5333
uM
 48
hr
 1 1376 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 121.03 NGFR rabbit IgG(H+L) / donkey NGFR 1089.00
WM115-B1-rep1
F8
WM-115 Vemurafenib RG-108 1.0000 0.5333
uM
 48
hr
 1 1156 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 157.47 NGFR rabbit IgG(H+L) / donkey NGFR 1016.92
WM115-B1-rep1
G2
WM-115 Vemurafenib Anacardic acid 1.0000 0.5333
uM
 48
hr
 1 1099 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 138.32 NGFR rabbit IgG(H+L) / donkey NGFR 1027.42
WM115-B1-rep1
G3
WM-115 Vemurafenib MC1568 1.0000 0.5333
uM
 48
hr
 1 1071 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 186.41 NGFR rabbit IgG(H+L) / donkey NGFR 1029.34
WM115-B1-rep1
G4
WM-115 Vemurafenib UNC0638 1.0000 0.5333
uM
 48
hr
 1 1095 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 146.03 NGFR rabbit IgG(H+L) / donkey NGFR 1097.91
WM115-B1-rep1
G5
WM-115 Vemurafenib Tranylcypromine 1.0000 0.5333
uM
 48
hr
 1 1076 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 134.75 NGFR rabbit IgG(H+L) / donkey NGFR 1159.45
WM115-B1-rep1
G6
WM-115 Vemurafenib Ischemin 1.0000 0.5333
uM
 48
hr
 1 986 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 132.26 NGFR rabbit IgG(H+L) / donkey NGFR 1032.60
WM115-B1-rep1
G7
WM-115 Vemurafenib 1.0000
uM
 48
hr
 1 1136 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 140.45 NGFR rabbit IgG(H+L) / donkey NGFR 946.39
WM115-B1-rep1
H10
WM-115 Vemurafenib 1.0000
uM
 48
hr
 1 976 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 163.14 NGFR rabbit IgG(H+L) / donkey NGFR 952.48
WM115-B1-rep1
H11
WM-115 Vemurafenib 1.0000
uM
 48
hr
 1 1076 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 143.73 NGFR rabbit IgG(H+L) / donkey NGFR 961.85
WM115-B1-rep1
H2
WM-115 Vemurafenib 1.0000
uM
 48
hr
 1 966 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 123.08 NGFR rabbit IgG(H+L) / donkey NGFR 1010.70
WM115-B1-rep1
H3
WM-115 Vemurafenib 1.0000
uM
 48
hr
 1 954 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 126.62 NGFR rabbit IgG(H+L) / donkey NGFR 1061.42
WM115-B1-rep1
H4
WM-115 Vemurafenib 1.0000
uM
 48
hr
 1 999 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 126.60 NGFR rabbit IgG(H+L) / donkey NGFR 1041.89
WM115-B1-rep1
H5
WM-115 Vemurafenib 1.0000
uM
 48
hr
 1 971 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 144.64 NGFR rabbit IgG(H+L) / donkey NGFR 992.91
WM115-B1-rep1
H6
WM-115 Vemurafenib 1.0000
uM
 48
hr
 1 1020 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 135.56 NGFR rabbit IgG(H+L) / donkey NGFR 1013.45
WM115-B1-rep1
H7
WM-115 Vemurafenib 1.0000
uM
 48
hr
 1 971 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 138.87 NGFR rabbit IgG(H+L) / donkey NGFR 1060.23
WM115-B1-rep1
H8
WM-115 Vemurafenib 1.0000
uM
 48
hr
 1 1086 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 138.77 NGFR rabbit IgG(H+L) / donkey NGFR 1029.13
WM115-B1-rep1
H9
WM-115 Vemurafenib 1.0000
uM
 48
hr
 2 1307 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 155.08 NGFR rabbit IgG(H+L) / donkey NGFR 1224.07
WM115-B1-rep2
A10