Protein levels measured by imaging in BRAF(V600E/D) melanoma cell lines following treatment with Vemurafenib in combination with chromatin-targeting compounds (immunofluorescence dataset 3 of 4) - Dataset (ID:20276)

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Cell Small Molecule 1 Small Molecule 2 Small Mol 1 Concentration Small Mol 2 Concentration Small Mol Conc Unit Time Point Time Point Unit Replicate Number of Cells Primary Antibody (488) Secondary Antibody (488) Protein Detected (488) Mean Fluorescence Intensity (488) Primary Antibody (647) Secondary Antibody (647) Protein Detected (647) Mean Fluorescence Intensity (647) Plate Name Plate Well
A-375 Vemurafenib 1.0000
uM
 48
hr
 2 2312 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 193.78 NGFR rabbit IgG(H+L) / donkey NGFR 179.41
A375-B1-rep2
A3
A-375 Vemurafenib 1.0000
uM
 48
hr
 2 2108 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 195.39 NGFR rabbit IgG(H+L) / donkey NGFR 201.04
A375-B1-rep2
A2
A-375 Vemurafenib Azacitidine 1.0000 2.6667
uM
 48
hr
 2 1303 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 286.33 NGFR rabbit IgG(H+L) / donkey NGFR 62.73
A375-A2-rep2
E2
A-375 Vemurafenib 1.0000
uM
 48
hr
 2 1490 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 206.53 NGFR rabbit IgG(H+L) / donkey NGFR 194.20
A375-B1-rep2
A11
A-375 Vemurafenib 1.0000
uM
 48
hr
 2 1697 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 193.98 NGFR rabbit IgG(H+L) / donkey NGFR 177.83
A375-B1-rep2
A10
A-375 Vemurafenib IOX2 1.0000 2.6667
uM
 48
hr
 2 2998 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 182.38 NGFR rabbit IgG(H+L) / donkey NGFR 220.87
A375-A2-rep2
D8
A-375 Vemurafenib RG-108 1.0000 2.6667
uM
 48
hr
 1 1476 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 171.86 NGFR rabbit IgG(H+L) / donkey NGFR 162.39
A375-A2-rep1
G2
A-375 Vemurafenib (+)-JQ1 1.0000 2.6667
uM
 48
hr
 1 1957 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 124.74 NGFR rabbit IgG(H+L) / donkey NGFR 79.92
A375-A2-rep1
C7
A-375 Vemurafenib 1.0000
uM
 48
hr
 2 1514 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 164.99 NGFR rabbit IgG(H+L) / donkey NGFR 137.41
A375-B2-rep2
H9
A-375 Vemurafenib 1.0000
uM
 48
hr
 1 1314 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 167.73 NGFR rabbit IgG(H+L) / donkey NGFR 154.69
A375-B1-rep1
H9
A-375 Vemurafenib 1.0000
uM
 48
hr
 2 1439 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 142.21 NGFR rabbit IgG(H+L) / donkey NGFR 140.69
A375-B2-rep2
H8
A-375 Vemurafenib 1.0000
uM
 48
hr
 2 1348 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 158.69 NGFR rabbit IgG(H+L) / donkey NGFR 125.38
A375-B2-rep2
H7
A-375 Vemurafenib 1.0000
uM
 48
hr
 1 1111 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 143.94 NGFR rabbit IgG(H+L) / donkey NGFR 127.79
A375-B1-rep1
H8
A-375 Vemurafenib (-)-JQ1 1.0000 2.6667
uM
 48
hr
 2 3409 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 167.33 NGFR rabbit IgG(H+L) / donkey NGFR 293.21
A375-A2-rep2
D7
A-375 Vemurafenib 1.0000
uM
 48
hr
 2 1391 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 157.37 NGFR rabbit IgG(H+L) / donkey NGFR 133.06
A375-B2-rep2
H6
A-375 Vemurafenib 1.0000
uM
 48
hr
 2 1377 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 149.79 NGFR rabbit IgG(H+L) / donkey NGFR 130.22
A375-B2-rep2
H5
A-375 Vemurafenib 1.0000
uM
 48
hr
 1 1386 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 162.16 NGFR rabbit IgG(H+L) / donkey NGFR 141.90
A375-B1-rep1
H7
A-375 Vemurafenib 1.0000
uM
 48
hr
 2 1560 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 133.12 NGFR rabbit IgG(H+L) / donkey NGFR 135.12
A375-B2-rep2
H4
A-375 Vemurafenib 1.0000
uM
 48
hr
 2 1568 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 173.46 NGFR rabbit IgG(H+L) / donkey NGFR 132.48
A375-B2-rep2
H3
A-375 Vemurafenib 1.0000
uM
 48
hr
 1 1289 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 162.79 NGFR rabbit IgG(H+L) / donkey NGFR 134.80
A375-B1-rep1
H6
A-375 Vemurafenib GSK-J4 1.0000 2.6667
uM
 48
hr
 2 1738 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 131.40 NGFR rabbit IgG(H+L) / donkey NGFR 104.43
A375-A2-rep2
D6
A-375 Vemurafenib CX-5461 1.0000 2.6667
uM
 48
hr
 1 1229 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 146.16 NGFR rabbit IgG(H+L) / donkey NGFR 147.71
A375-A2-rep1
F8
A-375 Vemurafenib 1.0000
uM
 48
hr
 2 1626 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 158.56 NGFR rabbit IgG(H+L) / donkey NGFR 142.15
A375-B2-rep2
H2
A-375 Vemurafenib 1.0000
uM
 48
hr
 2 1564 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 129.45 NGFR rabbit IgG(H+L) / donkey NGFR 110.35
A375-B2-rep2
H11
A-375 Vemurafenib 1.0000
uM
 48
hr
 1 1235 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 168.84 NGFR rabbit IgG(H+L) / donkey NGFR 135.45
A375-B1-rep1
H5