Protein levels measured by imaging in BRAF(V600E/D) melanoma cell lines following treatment with Vemurafenib in combination with chromatin-targeting compounds (immunofluorescence dataset 3 of 4) - Dataset (ID:20276)

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Cell Small Molecule 1 Small Molecule 2 Small Mol 1 Concentration Small Mol 2 Concentration Small Mol Conc Unit Time Point Time Point Unit Replicate Number of Cells Primary Antibody (488) Secondary Antibody (488) Protein Detected (488) Mean Fluorescence Intensity (488) Primary Antibody (647) Secondary Antibody (647) Protein Detected (647) Mean Fluorescence Intensity (647) Plate Name Plate Well
A-375 Vemurafenib PFI-1 1.0000 0.5333
uM
 48
hr
 2 2340 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 155.42 NGFR rabbit IgG(H+L) / donkey NGFR 204.08
A375-B1-rep2
B7
A-375 Vemurafenib GSK-J1 1.0000 0.5333
uM
 48
hr
 2 2749 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 153.27 NGFR rabbit IgG(H+L) / donkey NGFR 180.25
A375-B1-rep2
B6
A-375 Vemurafenib Epigallocatechin gallate 1.0000 2.6667
uM
 48
hr
 2 2588 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 185.72 NGFR rabbit IgG(H+L) / donkey NGFR 175.22
A375-A2-rep2
E8
A-375 Vemurafenib Rocilinostat 1.0000 0.5333
uM
 48
hr
 2 2904 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 288.39 NGFR rabbit IgG(H+L) / donkey NGFR 128.70
A375-B1-rep2
B5
A-375 Vemurafenib CTB 1.0000 0.5333
uM
 48
hr
 2 2694 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 159.97 NGFR rabbit IgG(H+L) / donkey NGFR 139.51
A375-B1-rep2
B4
A-375 Vemurafenib I-BET 1.0000 2.6667
uM
 48
hr
 2 2493 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 130.30 NGFR rabbit IgG(H+L) / donkey NGFR 93.88
A375-A2-rep2
E7
A-375 Vemurafenib UNC0638 1.0000 2.6667
uM
 48
hr
 1 1266 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 181.34 NGFR rabbit IgG(H+L) / donkey NGFR 57.07
A375-A2-rep1
G5
A-375 Vemurafenib Iniparib 1.0000 0.5333
uM
 48
hr
 2 2392 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 155.20 NGFR rabbit IgG(H+L) / donkey NGFR 165.74
A375-B1-rep2
B3
A-375 Vemurafenib Olaparib 1.0000 0.5333
uM
 48
hr
 2 2162 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 173.14 NGFR rabbit IgG(H+L) / donkey NGFR 197.62
A375-B1-rep2
B2
A-375 Vemurafenib Daminozide 1.0000 2.6667
uM
 48
hr
 2 2889 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 184.32 NGFR rabbit IgG(H+L) / donkey NGFR 208.04
A375-A2-rep2
E6
A-375 Vemurafenib 1.0000
uM
 48
hr
 2 1958 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 162.65 NGFR rabbit IgG(H+L) / donkey NGFR 176.09
A375-B1-rep2
A9
A-375 Vemurafenib 1.0000
uM
 48
hr
 2 2194 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 176.74 NGFR rabbit IgG(H+L) / donkey NGFR 173.12
A375-B1-rep2
A8
A-375 Vemurafenib Resveratrol 1.0000 2.6667
uM
 48
hr
 2 2903 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 213.67 NGFR rabbit IgG(H+L) / donkey NGFR 191.15
A375-A2-rep2
E5
A-375 Vemurafenib MC1568 1.0000 2.6667
uM
 48
hr
 1 1904 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 389.68 NGFR rabbit IgG(H+L) / donkey NGFR 146.07
A375-A2-rep1
G4
A-375 Vemurafenib UNC1215 1.0000 2.6667
uM
 48
hr
 1 2539 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 153.45 NGFR rabbit IgG(H+L) / donkey NGFR 217.23
A375-A2-rep1
C8
A-375 Vemurafenib Iniparib 1.0000 2.6667
uM
 48
hr
 1 2408 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 180.32 NGFR rabbit IgG(H+L) / donkey NGFR 193.86
A375-A2-rep1
B3
A-375 Vemurafenib 1.0000
uM
 48
hr
 1 2252 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 227.51 NGFR rabbit IgG(H+L) / donkey NGFR 199.22
A375-A2-rep1
A5
A-375 Vemurafenib 1.0000
uM
 48
hr
 1 2214 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 229.79 NGFR rabbit IgG(H+L) / donkey NGFR 205.29
A375-A2-rep1
A2
A-375 Vemurafenib 1.0000
uM
 48
hr
 2 1975 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 186.12 NGFR rabbit IgG(H+L) / donkey NGFR 191.67
A375-B1-rep2
A7
A-375 Vemurafenib 1.0000
uM
 48
hr
 2 2302 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 198.28 NGFR rabbit IgG(H+L) / donkey NGFR 170.70
A375-B1-rep2
A6
A-375 Vemurafenib Mocetinostat 1.0000 2.6667
uM
 48
hr
 2 533 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 254.36 NGFR rabbit IgG(H+L) / donkey NGFR 81.09
A375-A2-rep2
E4
A-375 Vemurafenib 1.0000
uM
 48
hr
 2 1701 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 187.13 NGFR rabbit IgG(H+L) / donkey NGFR 188.45
A375-B1-rep2
A5
A-375 Vemurafenib 1.0000
uM
 48
hr
 2 2208 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 193.23 NGFR rabbit IgG(H+L) / donkey NGFR 197.15
A375-B1-rep2
A4
A-375 Vemurafenib C646 1.0000 2.6667
uM
 48
hr
 2 2465 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 167.50 NGFR rabbit IgG(H+L) / donkey NGFR 173.88
A375-A2-rep2
E3
A-375 Vemurafenib Anacardic acid 1.0000 2.6667
uM
 48
hr
 1 1318 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 145.31 NGFR rabbit IgG(H+L) / donkey NGFR 170.13
A375-A2-rep1
G3