Protein levels measured by imaging in BRAF(V600E/D) melanoma cell lines following treatment with Vemurafenib in combination with chromatin-targeting compounds (immunofluorescence dataset 3 of 4) - Dataset (ID:20276)

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Cell Small Molecule 1 Small Molecule 2 Small Mol 1 Concentration Small Mol 2 Concentration Small Mol Conc Unit Time Point Time Point Unit Replicate Number of Cells Primary Antibody (488) Secondary Antibody (488) Protein Detected (488) Mean Fluorescence Intensity (488) Primary Antibody (647) Secondary Antibody (647) Protein Detected (647) Mean Fluorescence Intensity (647) Plate Name Plate Well
COLO 858 Vemurafenib Epigallocatechin gallate 0.3160 2.6667
uM
 48
hr
 1 3105 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 357.79 NGFR rabbit IgG(H+L) / donkey NGFR 132.41
Colo858-A2-rep1
E8
COLO 858 Vemurafenib Pracinostat 0.3160 2.6667
uM
 48
hr
 1 169 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 222.43 NGFR rabbit IgG(H+L) / donkey NGFR 28.52
Colo858-A2-rep1
F4
COLO 858 Vemurafenib BIX-01294 0.3160 2.6667
uM
 48
hr
 1 561 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 189.27 NGFR rabbit IgG(H+L) / donkey NGFR 24.74
Colo858-A2-rep1
F5
COLO 858 Vemurafenib I-BET151 0.3160 2.6667
uM
 48
hr
 1 1486 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 118.87 NGFR rabbit IgG(H+L) / donkey NGFR 7.82
Colo858-A2-rep1
F7
COLO 858 Vemurafenib CX-5461 0.3160 2.6667
uM
 48
hr
 1 1891 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 209.48 NGFR rabbit IgG(H+L) / donkey NGFR 203.21
Colo858-A2-rep1
F8
COLO 858 Vemurafenib 0.3160
uM
 48
hr
 1 1391 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 358.32 NGFR rabbit IgG(H+L) / donkey NGFR 158.87
Colo858-A2-rep1
A10
COLO 858 Vemurafenib 0.3160
uM
 48
hr
 1 1242 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 296.85 NGFR rabbit IgG(H+L) / donkey NGFR 156.51
Colo858-A2-rep1
A11
COLO 858 Vemurafenib 0.3160
uM
 48
hr
 1 1024 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 345.50 NGFR rabbit IgG(H+L) / donkey NGFR 135.78
Colo858-A2-rep1
A3
COLO 858 Vemurafenib 0.3160
uM
 48
hr
 1 1076 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 288.18 NGFR rabbit IgG(H+L) / donkey NGFR 162.29
Colo858-A2-rep1
A4
COLO 858 Vemurafenib 0.3160
uM
 48
hr
 1 1095 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 337.39 NGFR rabbit IgG(H+L) / donkey NGFR 164.33
Colo858-A2-rep1
A7
COLO 858 Vemurafenib 0.3160
uM
 48
hr
 1 897 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 335.21 NGFR rabbit IgG(H+L) / donkey NGFR 126.80
Colo858-A2-rep1
A8
COLO 858 Vemurafenib Olaparib 0.3160 2.6667
uM
 48
hr
 1 1345 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 290.82 NGFR rabbit IgG(H+L) / donkey NGFR 146.21
Colo858-A2-rep1
B2
COLO 858 Vemurafenib Iniparib 0.3160 2.6667
uM
 48
hr
 1 1703 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 310.30 NGFR rabbit IgG(H+L) / donkey NGFR 122.97
Colo858-A2-rep1
B3
COLO 858 Vemurafenib PFI-1 0.3160 2.6667
uM
 48
hr
 1 1383 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 138.34 NGFR rabbit IgG(H+L) / donkey NGFR 30.41
Colo858-A2-rep1
B7
COLO 858 Vemurafenib UNC669 0.3160 2.6667
uM
 48
hr
 1 2097 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 357.07 NGFR rabbit IgG(H+L) / donkey NGFR 157.23
Colo858-A2-rep1
B8
COLO 858 Vemurafenib Rucaparib 0.3160 2.6667
uM
 48
hr
 1 2594 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 255.48 NGFR rabbit IgG(H+L) / donkey NGFR 179.23
Colo858-A2-rep1
C3
COLO 858 Vemurafenib Belinostat 0.3160 2.6667
uM
 48
hr
 1 209 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 309.25 NGFR rabbit IgG(H+L) / donkey NGFR 21.49
Colo858-A2-rep1
C4
COLO 858 Vemurafenib (+)-JQ1 0.3160 2.6667
uM
 48
hr
 1 1745 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 123.39 NGFR rabbit IgG(H+L) / donkey NGFR 10.46
Colo858-A2-rep1
C7
COLO 858 Vemurafenib UNC1215 0.3160 2.6667
uM
 48
hr
 1 2700 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 375.51 NGFR rabbit IgG(H+L) / donkey NGFR 134.85
Colo858-A2-rep1
C8
COLO 858 Vemurafenib JW55 0.3160 2.6667
uM
 48
hr
 1 2243 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 243.79 NGFR rabbit IgG(H+L) / donkey NGFR 100.13
Colo858-A2-rep1
D3
COLO 858 Vemurafenib Entinostat 0.3160 2.6667
uM
 48
hr
 1 2256 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 128.46 NGFR rabbit IgG(H+L) / donkey NGFR 15.79
Colo858-A2-rep1
D4
COLO 858 Vemurafenib Azacitidine 0.3160 2.6667
uM
 48
hr
 1 840 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 198.97 NGFR rabbit IgG(H+L) / donkey NGFR 20.08
Colo858-A2-rep1
E2
COLO 858 Vemurafenib C646 0.3160 2.6667
uM
 48
hr
 1 2689 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 316.57 NGFR rabbit IgG(H+L) / donkey NGFR 154.25
Colo858-A2-rep1
E3
COLO 858 Vemurafenib Tranylcypromine 0.3160 2.6667
uM
 48
hr
 1 1165 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 304.43 NGFR rabbit IgG(H+L) / donkey NGFR 172.86
Colo858-A2-rep1
G6
COLO 858 Vemurafenib UNC0638 0.3160 2.6667
uM
 48
hr
 1 581 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 215.25 NGFR rabbit IgG(H+L) / donkey NGFR 98.55
Colo858-A2-rep1
G5