Protein levels measured by imaging in BRAF(V600E/D) melanoma cell lines following treatment with Vemurafenib in combination with chromatin-targeting compounds (immunofluorescence dataset 3 of 4) - Dataset (ID:20276)

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Cell Small Molecule 1 Small Molecule 2 Small Mol 1 Concentration Small Mol 2 Concentration Small Mol Conc Unit Time Point Time Point Unit Replicate Number of Cells Primary Antibody (488) Secondary Antibody (488) Protein Detected (488) Mean Fluorescence Intensity (488) Primary Antibody (647) Secondary Antibody (647) Protein Detected (647) Mean Fluorescence Intensity (647) Plate Name Plate Well
COLO 858 Vemurafenib AGK2 0.3160 2.6667
uM
 48
hr
 2 635 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 266.45 NGFR rabbit IgG(H+L) / donkey NGFR 71.47
Colo858-A2-rep2
D5
COLO 858 Vemurafenib GSK-J4 0.3160 2.6667
uM
 48
hr
 2 682 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 189.00 NGFR rabbit IgG(H+L) / donkey NGFR 11.65
Colo858-A2-rep2
D6
COLO 858 Vemurafenib Mocetinostat 0.3160 2.6667
uM
 48
hr
 2 2331 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 165.13 NGFR rabbit IgG(H+L) / donkey NGFR 22.79
Colo858-A2-rep2
E4
COLO 858 Vemurafenib Resveratrol 0.3160 2.6667
uM
 48
hr
 2 2640 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 313.76 NGFR rabbit IgG(H+L) / donkey NGFR 122.85
Colo858-A2-rep2
E5
COLO 858 Vemurafenib I-BET 0.3160 2.6667
uM
 48
hr
 2 2077 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 129.89 NGFR rabbit IgG(H+L) / donkey NGFR 10.47
Colo858-A2-rep2
E7
COLO 858 Vemurafenib Epigallocatechin gallate 0.3160 2.6667
uM
 48
hr
 2 2922 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 321.58 NGFR rabbit IgG(H+L) / donkey NGFR 130.77
Colo858-A2-rep2
E8
COLO 858 Vemurafenib Pracinostat 0.3160 2.6667
uM
 48
hr
 2 127 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 200.69 NGFR rabbit IgG(H+L) / donkey NGFR 37.13
Colo858-A2-rep2
F4
COLO 858 Vemurafenib BIX-01294 0.3160 2.6667
uM
 48
hr
 2 721 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 172.48 NGFR rabbit IgG(H+L) / donkey NGFR 30.74
Colo858-A2-rep2
F5
COLO 858 Vemurafenib I-BET151 0.3160 2.6667
uM
 48
hr
 2 1438 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 110.13 NGFR rabbit IgG(H+L) / donkey NGFR 13.48
Colo858-A2-rep2
F7
COLO 858 Vemurafenib CX-5461 0.3160 2.6667
uM
 48
hr
 2 1927 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 203.59 NGFR rabbit IgG(H+L) / donkey NGFR 217.19
Colo858-A2-rep2
F8
COLO 858 Vemurafenib UNC0638 0.3160 2.6667
uM
 48
hr
 2 793 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 188.46 NGFR rabbit IgG(H+L) / donkey NGFR 153.42
Colo858-A2-rep2
G5
COLO 858 Vemurafenib Tranylcypromine 0.3160 2.6667
uM
 48
hr
 2 1329 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 304.95 NGFR rabbit IgG(H+L) / donkey NGFR 179.43
Colo858-A2-rep2
G6
COLO 858 Vemurafenib 0.3160
uM
 48
hr
 2 901 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 241.75 NGFR rabbit IgG(H+L) / donkey NGFR 128.46
Colo858-A2-rep2
H10
COLO 858 Vemurafenib 0.3160
uM
 48
hr
 2 865 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 215.59 NGFR rabbit IgG(H+L) / donkey NGFR 149.37
Colo858-A2-rep2
H11
COLO 858 Vemurafenib 0.3160
uM
 48
hr
 2 728 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 275.60 NGFR rabbit IgG(H+L) / donkey NGFR 129.02
Colo858-A2-rep2
H4
COLO 858 Vemurafenib 0.3160
uM
 48
hr
 2 818 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 270.37 NGFR rabbit IgG(H+L) / donkey NGFR 132.19
Colo858-A2-rep2
H5
COLO 858 Vemurafenib 0.3160
uM
 48
hr
 2 903 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 227.64 NGFR rabbit IgG(H+L) / donkey NGFR 138.37
Colo858-A2-rep2
H7
COLO 858 Vemurafenib 0.3160
uM
 48
hr
 2 922 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 278.87 NGFR rabbit IgG(H+L) / donkey NGFR 126.84
Colo858-A2-rep2
H8
COLO 858 Vemurafenib 0.3160
uM
 48
hr
 1 1008 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 371.10 NGFR rabbit IgG(H+L) / donkey NGFR 120.59
Colo858-B1-rep1
A5
COLO 858 Vemurafenib 0.3160
uM
 48
hr
 1 1005 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 348.84 NGFR rabbit IgG(H+L) / donkey NGFR 143.10
Colo858-B1-rep1
A6
COLO 858 Vemurafenib 0.3160
uM
 48
hr
 1 970 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 376.63 NGFR rabbit IgG(H+L) / donkey NGFR 125.61
Colo858-B1-rep1
A8
COLO 858 Vemurafenib 0.3160
uM
 48
hr
 1 1228 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 378.70 NGFR rabbit IgG(H+L) / donkey NGFR 125.18
Colo858-B1-rep1
A9
COLO 858 Vemurafenib CTB 0.3160 0.5333
uM
 48
hr
 1 1883 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 274.67 NGFR rabbit IgG(H+L) / donkey NGFR 153.12
Colo858-B1-rep1
B4
COLO 858 Vemurafenib Belinostat 0.3160 0.1067
uM
 48
hr
 2 796 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 190.45 NGFR rabbit IgG(H+L) / donkey NGFR 129.48
Colo858-B2-rep2
C4
COLO 858 Vemurafenib Rucaparib 0.3160 0.1067
uM
 48
hr
 2 2779 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 314.05 NGFR rabbit IgG(H+L) / donkey NGFR 173.73
Colo858-B2-rep2
C3