Protein levels measured by imaging in BRAF(V600E/D) melanoma cell lines following treatment with Vemurafenib in combination with chromatin-targeting compounds (immunofluorescence dataset 3 of 4) - Dataset (ID:20276)

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Cell Small Molecule 1 Small Molecule 2 Small Mol 1 Concentration Small Mol 2 Concentration Small Mol Conc Unit Time Point Time Point Unit Replicate Number of Cells Primary Antibody (488) Secondary Antibody (488) Protein Detected (488) Mean Fluorescence Intensity (488) Primary Antibody (647) Secondary Antibody (647) Protein Detected (647) Mean Fluorescence Intensity (647) Plate Name Plate Well
COLO 858 Vemurafenib Garcinol 0.3160 2.6667
uM
 48
hr
 1 981 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 330.96 NGFR rabbit IgG(H+L) / donkey NGFR 147.82
Colo858-A2-rep1
F3
COLO 858 Vemurafenib BIX-01294 0.3160 2.6667
uM
 48
hr
 1 561 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 189.27 NGFR rabbit IgG(H+L) / donkey NGFR 24.74
Colo858-A2-rep1
F5
COLO 858 Vemurafenib Methylstat 0.3160 2.6667
uM
 48
hr
 1 1783 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 213.42 NGFR rabbit IgG(H+L) / donkey NGFR 68.18
Colo858-A2-rep1
F6
COLO 858 Vemurafenib Anacardic acid 0.3160 2.6667
uM
 48
hr
 1 1253 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 315.28 NGFR rabbit IgG(H+L) / donkey NGFR 120.19
Colo858-A2-rep1
G3
COLO 858 Vemurafenib MC1568 0.3160 2.6667
uM
 48
hr
 1 1318 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 365.04 NGFR rabbit IgG(H+L) / donkey NGFR 114.40
Colo858-A2-rep1
G4
COLO 858 Vemurafenib Tranylcypromine 0.3160 2.6667
uM
 48
hr
 1 1165 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 304.43 NGFR rabbit IgG(H+L) / donkey NGFR 172.86
Colo858-A2-rep1
G6
COLO 858 Vemurafenib Ischemin 0.3160 2.6667
uM
 48
hr
 1 1323 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 307.48 NGFR rabbit IgG(H+L) / donkey NGFR 114.56
Colo858-A2-rep1
G7
COLO 858 Vemurafenib 0.3160
uM
 48
hr
 1 964 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 364.09 NGFR rabbit IgG(H+L) / donkey NGFR 110.85
Colo858-A2-rep1
H2
COLO 858 Vemurafenib 0.3160
uM
 48
hr
 1 950 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 327.98 NGFR rabbit IgG(H+L) / donkey NGFR 130.86
Colo858-A2-rep1
H3
COLO 858 Vemurafenib 0.3160
uM
 48
hr
 1 673 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 276.26 NGFR rabbit IgG(H+L) / donkey NGFR 130.08
Colo858-A2-rep1
H5
COLO 858 Vemurafenib 0.3160
uM
 48
hr
 1 797 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 303.09 NGFR rabbit IgG(H+L) / donkey NGFR 127.30
Colo858-A2-rep1
H6
COLO 858 Vemurafenib 0.3160
uM
 48
hr
 2 1033 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 359.83 NGFR rabbit IgG(H+L) / donkey NGFR 196.89
Colo858-A2-rep2
A2
COLO 858 Vemurafenib 0.3160
uM
 48
hr
 2 908 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 313.42 NGFR rabbit IgG(H+L) / donkey NGFR 130.36
Colo858-A2-rep2
A3
COLO 858 Vemurafenib 0.3160
uM
 48
hr
 2 1117 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 406.39 NGFR rabbit IgG(H+L) / donkey NGFR 145.48
Colo858-A2-rep2
A5
COLO 858 Vemurafenib 0.3160
uM
 48
hr
 2 923 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 349.90 NGFR rabbit IgG(H+L) / donkey NGFR 131.92
Colo858-A2-rep2
A6
COLO 858 Vemurafenib 0.3160
uM
 48
hr
 2 965 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 322.70 NGFR rabbit IgG(H+L) / donkey NGFR 158.50
Colo858-A2-rep2
A9
COLO 858 Vemurafenib Olaparib 0.3160 2.6667
uM
 48
hr
 2 1429 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 244.86 NGFR rabbit IgG(H+L) / donkey NGFR 158.62
Colo858-A2-rep2
B2
COLO 858 Vemurafenib CTB 0.3160 2.6667
uM
 48
hr
 2 1525 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 264.79 NGFR rabbit IgG(H+L) / donkey NGFR 156.84
Colo858-A2-rep2
B4
COLO 858 Vemurafenib Rocilinostat 0.3160 2.6667
uM
 48
hr
 2 596 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 175.51 NGFR rabbit IgG(H+L) / donkey NGFR 157.64
Colo858-A2-rep2
B5
COLO 858 Vemurafenib Veliparib 0.3160 2.6667
uM
 48
hr
 2 1852 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 303.91 NGFR rabbit IgG(H+L) / donkey NGFR 148.05
Colo858-A2-rep2
C2
COLO 858 Vemurafenib Rucaparib 0.3160 2.6667
uM
 48
hr
 2 2191 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 274.88 NGFR rabbit IgG(H+L) / donkey NGFR 168.71
Colo858-A2-rep2
C3
COLO 858 Vemurafenib Selisistat 0.3160 2.6667
uM
 48
hr
 2 2435 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 300.72 NGFR rabbit IgG(H+L) / donkey NGFR 104.24
Colo858-A2-rep2
C5
COLO 858 Vemurafenib GSK-J2 0.3160 2.6667
uM
 48
hr
 2 2739 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 331.72 NGFR rabbit IgG(H+L) / donkey NGFR 152.80
Colo858-A2-rep2
C6
COLO 858 Vemurafenib 0.3160
uM
 48
hr
 1 748 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 229.27 NGFR rabbit IgG(H+L) / donkey NGFR 160.07
Colo858-B2-rep1
H4
COLO 858 Vemurafenib 0.3160
uM
 48
hr
 1 709 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 278.92 NGFR rabbit IgG(H+L) / donkey NGFR 116.26
Colo858-B2-rep1
H3