Protein levels measured by imaging in BRAF(V600E/D) melanoma cell lines following treatment with Vemurafenib in combination with chromatin-targeting compounds (immunofluorescence dataset 3 of 4) - Dataset (ID:20276)

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Cell Small Molecule 1 Small Molecule 2 Small Mol 1 Concentration Small Mol 2 Concentration Small Mol Conc Unit Time Point Time Point Unit Replicate Number of Cells Primary Antibody (488) Secondary Antibody (488) Protein Detected (488) Mean Fluorescence Intensity (488) Primary Antibody (647) Secondary Antibody (647) Protein Detected (647) Mean Fluorescence Intensity (647) Plate Name Plate Well
COLO 858 Vemurafenib Rucaparib 0.3160 0.1067
uM
 48
hr
 1 2092 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 264.74 NGFR rabbit IgG(H+L) / donkey NGFR 163.85
Colo858-B2-rep1
C3
COLO 858 Vemurafenib Belinostat 0.3160 0.1067
uM
 48
hr
 1 798 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 187.52 NGFR rabbit IgG(H+L) / donkey NGFR 66.34
Colo858-B2-rep1
C4
COLO 858 Vemurafenib GSK-J2 0.3160 0.1067
uM
 48
hr
 1 2462 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 317.12 NGFR rabbit IgG(H+L) / donkey NGFR 150.12
Colo858-B2-rep1
C6
COLO 858 Vemurafenib (+)-JQ1 0.3160 0.1067
uM
 48
hr
 1 1564 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 110.51 NGFR rabbit IgG(H+L) / donkey NGFR 23.82
Colo858-B2-rep1
C7
COLO 858 Vemurafenib 0.3160
uM
 48
hr
 1 1391 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 358.32 NGFR rabbit IgG(H+L) / donkey NGFR 158.87
Colo858-A2-rep1
A10
COLO 858 Vemurafenib 0.3160
uM
 48
hr
 1 1242 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 296.85 NGFR rabbit IgG(H+L) / donkey NGFR 156.51
Colo858-A2-rep1
A11
COLO 858 Vemurafenib 0.3160
uM
 48
hr
 1 1024 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 345.50 NGFR rabbit IgG(H+L) / donkey NGFR 135.78
Colo858-A2-rep1
A3
COLO 858 Vemurafenib 0.3160
uM
 48
hr
 1 1076 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 288.18 NGFR rabbit IgG(H+L) / donkey NGFR 162.29
Colo858-A2-rep1
A4
COLO 858 Vemurafenib 0.3160
uM
 48
hr
 1 1095 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 337.39 NGFR rabbit IgG(H+L) / donkey NGFR 164.33
Colo858-A2-rep1
A7
COLO 858 Vemurafenib 0.3160
uM
 48
hr
 1 897 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 335.21 NGFR rabbit IgG(H+L) / donkey NGFR 126.80
Colo858-A2-rep1
A8
COLO 858 Vemurafenib Olaparib 0.3160 2.6667
uM
 48
hr
 1 1345 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 290.82 NGFR rabbit IgG(H+L) / donkey NGFR 146.21
Colo858-A2-rep1
B2
COLO 858 Vemurafenib Iniparib 0.3160 2.6667
uM
 48
hr
 1 1703 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 310.30 NGFR rabbit IgG(H+L) / donkey NGFR 122.97
Colo858-A2-rep1
B3
COLO 858 Vemurafenib PFI-1 0.3160 2.6667
uM
 48
hr
 1 1383 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 138.34 NGFR rabbit IgG(H+L) / donkey NGFR 30.41
Colo858-A2-rep1
B7
COLO 858 Vemurafenib UNC669 0.3160 2.6667
uM
 48
hr
 1 2097 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 357.07 NGFR rabbit IgG(H+L) / donkey NGFR 157.23
Colo858-A2-rep1
B8
COLO 858 Vemurafenib Rucaparib 0.3160 2.6667
uM
 48
hr
 1 2594 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 255.48 NGFR rabbit IgG(H+L) / donkey NGFR 179.23
Colo858-A2-rep1
C3
COLO 858 Vemurafenib Belinostat 0.3160 2.6667
uM
 48
hr
 1 209 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 309.25 NGFR rabbit IgG(H+L) / donkey NGFR 21.49
Colo858-A2-rep1
C4
COLO 858 Vemurafenib (+)-JQ1 0.3160 2.6667
uM
 48
hr
 1 1745 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 123.39 NGFR rabbit IgG(H+L) / donkey NGFR 10.46
Colo858-A2-rep1
C7
COLO 858 Vemurafenib UNC1215 0.3160 2.6667
uM
 48
hr
 1 2700 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 375.51 NGFR rabbit IgG(H+L) / donkey NGFR 134.85
Colo858-A2-rep1
C8
COLO 858 Vemurafenib JW55 0.3160 2.6667
uM
 48
hr
 1 2243 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 243.79 NGFR rabbit IgG(H+L) / donkey NGFR 100.13
Colo858-A2-rep1
D3
COLO 858 Vemurafenib Entinostat 0.3160 2.6667
uM
 48
hr
 1 2256 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 128.46 NGFR rabbit IgG(H+L) / donkey NGFR 15.79
Colo858-A2-rep1
D4
COLO 858 Vemurafenib Azacitidine 0.3160 2.6667
uM
 48
hr
 1 840 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 198.97 NGFR rabbit IgG(H+L) / donkey NGFR 20.08
Colo858-A2-rep1
E2
COLO 858 Vemurafenib C646 0.3160 2.6667
uM
 48
hr
 1 2689 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 316.57 NGFR rabbit IgG(H+L) / donkey NGFR 154.25
Colo858-A2-rep1
E3
COLO 858 Vemurafenib Resveratrol 0.3160 2.6667
uM
 48
hr
 1 2795 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 318.85 NGFR rabbit IgG(H+L) / donkey NGFR 129.75
Colo858-A2-rep1
E5
COLO 858 Vemurafenib IOX2 0.3160 0.1067
uM
 48
hr
 1 3247 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 296.22 NGFR rabbit IgG(H+L) / donkey NGFR 170.23
Colo858-B2-rep1
D8
COLO 858 Vemurafenib (-)-JQ1 0.3160 0.1067
uM
 48
hr
 1 3394 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 313.83 NGFR rabbit IgG(H+L) / donkey NGFR 168.87
Colo858-B2-rep1
D7