Protein levels measured by imaging in BRAF(V600E/D) melanoma cell lines following treatment with Vemurafenib in combination with chromatin-targeting compounds (immunofluorescence dataset 3 of 4) - Dataset (ID:20276)

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Cell Small Molecule 1 Small Molecule 2 Small Mol 1 Concentration Small Mol 2 Concentration Small Mol Conc Unit Time Point Time Point Unit Replicate Number of Cells Primary Antibody (488) Secondary Antibody (488) Protein Detected (488) Mean Fluorescence Intensity (488) Primary Antibody (647) Secondary Antibody (647) Protein Detected (647) Mean Fluorescence Intensity (647) Plate Name Plate Well
COLO 858 Vemurafenib Iniparib 0.3160 0.5333
uM
 48
hr
 2 1475 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 300.07 NGFR rabbit IgG(H+L) / donkey NGFR 155.24
Colo858-B1-rep2
B3
COLO 858 Vemurafenib GSK-J1 0.3160 0.5333
uM
 48
hr
 2 1597 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 332.46 NGFR rabbit IgG(H+L) / donkey NGFR 143.42
Colo858-B1-rep2
B6
COLO 858 Vemurafenib PFI-1 0.3160 0.5333
uM
 48
hr
 2 1454 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 310.50 NGFR rabbit IgG(H+L) / donkey NGFR 102.50
Colo858-B1-rep2
B7
COLO 858 Vemurafenib Veliparib 0.3160 0.5333
uM
 48
hr
 2 1765 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 252.26 NGFR rabbit IgG(H+L) / donkey NGFR 154.63
Colo858-B1-rep2
C2
COLO 858 Vemurafenib Rucaparib 0.3160 0.5333
uM
 48
hr
 2 2053 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 276.51 NGFR rabbit IgG(H+L) / donkey NGFR 162.63
Colo858-B1-rep2
C3
COLO 858 Vemurafenib (+)-JQ1 0.3160 0.5333
uM
 48
hr
 2 1702 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 111.67 NGFR rabbit IgG(H+L) / donkey NGFR 14.18
Colo858-B1-rep2
C7
COLO 858 Vemurafenib UNC1215 0.3160 0.5333
uM
 48
hr
 2 2388 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 298.81 NGFR rabbit IgG(H+L) / donkey NGFR 149.60
Colo858-B1-rep2
C8
COLO 858 Vemurafenib JW55 0.3160 0.5333
uM
 48
hr
 2 2472 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 267.42 NGFR rabbit IgG(H+L) / donkey NGFR 137.02
Colo858-B1-rep2
D3
COLO 858 Vemurafenib Entinostat 0.3160 0.5333
uM
 48
hr
 2 2701 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 211.88 NGFR rabbit IgG(H+L) / donkey NGFR 80.70
Colo858-B1-rep2
D4
COLO 858 Vemurafenib (-)-JQ1 0.3160 0.5333
uM
 48
hr
 2 3387 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 288.83 NGFR rabbit IgG(H+L) / donkey NGFR 150.04
Colo858-B1-rep2
D7
COLO 858 Vemurafenib IOX2 0.3160 0.5333
uM
 48
hr
 2 3074 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 323.98 NGFR rabbit IgG(H+L) / donkey NGFR 143.82
Colo858-B1-rep2
D8
COLO 858 Vemurafenib C646 0.3160 0.5333
uM
 48
hr
 2 2126 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 248.60 NGFR rabbit IgG(H+L) / donkey NGFR 140.35
Colo858-B1-rep2
E3
COLO 858 Vemurafenib Mocetinostat 0.3160 0.5333
uM
 48
hr
 2 2847 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 153.05 NGFR rabbit IgG(H+L) / donkey NGFR 41.69
Colo858-B1-rep2
E4
COLO 858 Vemurafenib Decitabine 0.3160 0.5333
uM
 48
hr
 2 1367 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 214.98 NGFR rabbit IgG(H+L) / donkey NGFR 173.73
Colo858-B1-rep2
F2
COLO 858 Vemurafenib Garcinol 0.3160 0.5333
uM
 48
hr
 2 1674 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 281.10 NGFR rabbit IgG(H+L) / donkey NGFR 142.39
Colo858-B1-rep2
F3
COLO 858 Vemurafenib BIX-01294 0.3160 0.5333
uM
 48
hr
 2 1928 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 358.98 NGFR rabbit IgG(H+L) / donkey NGFR 120.70
Colo858-B1-rep2
F5
COLO 858 Vemurafenib Methylstat 0.3160 0.5333
uM
 48
hr
 2 2202 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 242.84 NGFR rabbit IgG(H+L) / donkey NGFR 132.23
Colo858-B1-rep2
F6
COLO 858 Vemurafenib RG-108 0.3160 0.5333
uM
 48
hr
 2 1025 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 269.93 NGFR rabbit IgG(H+L) / donkey NGFR 130.25
Colo858-B1-rep2
G2
COLO 858 Vemurafenib Anacardic acid 0.3160 0.5333
uM
 48
hr
 2 926 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 272.48 NGFR rabbit IgG(H+L) / donkey NGFR 124.30
Colo858-B1-rep2
G3
COLO 858 Vemurafenib UNC0638 0.3160 0.5333
uM
 48
hr
 2 1278 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 262.32 NGFR rabbit IgG(H+L) / donkey NGFR 196.87
Colo858-B1-rep2
G5
COLO 858 Vemurafenib Tranylcypromine 0.3160 0.5333
uM
 48
hr
 2 1326 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 311.56 NGFR rabbit IgG(H+L) / donkey NGFR 177.03
Colo858-B1-rep2
G6
COLO 858 Vemurafenib 0.3160
uM
 48
hr
 2 787 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 272.73 NGFR rabbit IgG(H+L) / donkey NGFR 136.82
Colo858-B1-rep2
H2
COLO 858 Vemurafenib 0.3160
uM
 48
hr
 2 610 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 248.64 NGFR rabbit IgG(H+L) / donkey NGFR 141.66
Colo858-B1-rep2
H3
COLO 858 Vemurafenib 0.3160
uM
 48
hr
 1 892 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 319.64 NGFR rabbit IgG(H+L) / donkey NGFR 125.87
Colo858-B2-rep1
A5
COLO 858 Vemurafenib 0.3160
uM
 48
hr
 1 1027 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 328.61 NGFR rabbit IgG(H+L) / donkey NGFR 146.72
Colo858-B2-rep1
A4