Protein levels measured by imaging in BRAF(V600E/D) melanoma cell lines following treatment with Vemurafenib in combination with chromatin-targeting compounds (immunofluorescence dataset 3 of 4) - Dataset (ID:20276)

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Cell Small Molecule 1 Small Molecule 2 Small Mol 1 Concentration Small Mol 2 Concentration Small Mol Conc Unit Time Point Time Point Unit Replicate Number of Cells Primary Antibody (488) Secondary Antibody (488) Protein Detected (488) Mean Fluorescence Intensity (488) Primary Antibody (647) Secondary Antibody (647) Protein Detected (647) Mean Fluorescence Intensity (647) Plate Name Plate Well
COLO 858 Vemurafenib Veliparib 0.3160 0.1067
uM
 48
hr
 2 2385 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 352.61 NGFR rabbit IgG(H+L) / donkey NGFR 183.60
Colo858-B2-rep2
C2
COLO 858 Vemurafenib Veliparib 0.3160 0.1067
uM
 48
hr
 1 1792 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 277.98 NGFR rabbit IgG(H+L) / donkey NGFR 153.52
Colo858-B2-rep1
C2
COLO 858 Vemurafenib Veliparib 0.3160 0.5333
uM
 48
hr
 1 1919 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 252.38 NGFR rabbit IgG(H+L) / donkey NGFR 162.60
Colo858-B1-rep1
C2
A-375 Vemurafenib Veliparib 1.0000 0.1067
uM
 48
hr
 2 2173 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 161.93 NGFR rabbit IgG(H+L) / donkey NGFR 165.85
A375-B2-rep2
C2
WM-115 Vemurafenib Veliparib 1.0000 2.6667
uM
 48
hr
 2 1323 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 147.36 NGFR rabbit IgG(H+L) / donkey NGFR 1068.09
WM115-A2-rep2
C2
WM-115 Vemurafenib Veliparib 1.0000 2.6667
uM
 48
hr
 1 1299 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 149.15 NGFR rabbit IgG(H+L) / donkey NGFR 1076.34
WM115-A2-rep1
C2
COLO 858 Vemurafenib Veliparib 0.3160 2.6667
uM
 48
hr
 1 2017 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 297.02 NGFR rabbit IgG(H+L) / donkey NGFR 148.72
Colo858-A2-rep1
C2
A-375 Vemurafenib Veliparib 1.0000 2.6667
uM
 48
hr
 1 2081 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 171.06 NGFR rabbit IgG(H+L) / donkey NGFR 200.69
A375-A2-rep1
C2
WM-115 Vemurafenib Veliparib 1.0000 0.1067
uM
 48
hr
 1 1557 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 148.83 NGFR rabbit IgG(H+L) / donkey NGFR 1159.27
WM115-B2-rep1
C2
WM-115 Vemurafenib Veliparib 1.0000 0.5333
uM
 48
hr
 1 1336 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 145.75 NGFR rabbit IgG(H+L) / donkey NGFR 1058.87
WM115-B1-rep1
C2
WM-115 Vemurafenib Veliparib 1.0000 0.1067
uM
 48
hr
 2 1355 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 139.84 NGFR rabbit IgG(H+L) / donkey NGFR 1032.08
WM115-B2-rep2
C2
A-375 Vemurafenib UNC669 1.0000 0.5333
uM
 48
hr
 1 2511 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 175.77 NGFR rabbit IgG(H+L) / donkey NGFR 171.25
A375-B1-rep1
B8
A-375 Vemurafenib UNC669 1.0000 2.6667
uM
 48
hr
 2 2373 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 210.68 NGFR rabbit IgG(H+L) / donkey NGFR 209.48
A375-A2-rep2
B8
WM-115 Vemurafenib UNC669 1.0000 0.1067
uM
 48
hr
 1 1446 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 144.33 NGFR rabbit IgG(H+L) / donkey NGFR 1076.76
WM115-B2-rep1
B8
COLO 858 Vemurafenib UNC669 0.3160 0.1067
uM
 48
hr
 2 1966 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 419.20 NGFR rabbit IgG(H+L) / donkey NGFR 177.46
Colo858-B2-rep2
B8
COLO 858 Vemurafenib UNC669 0.3160 0.5333
uM
 48
hr
 2 1498 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 327.22 NGFR rabbit IgG(H+L) / donkey NGFR 143.43
Colo858-B1-rep2
B8
WM-115 Vemurafenib UNC669 1.0000 0.5333
uM
 48
hr
 2 1455 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 141.47 NGFR rabbit IgG(H+L) / donkey NGFR 1276.18
WM115-B1-rep2
B8
A-375 Vemurafenib UNC669 1.0000 0.1067
uM
 48
hr
 2 2020 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 138.07 NGFR rabbit IgG(H+L) / donkey NGFR 160.38
A375-B2-rep2
B8
COLO 858 Vemurafenib UNC669 0.3160 0.1067
uM
 48
hr
 1 1631 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 318.51 NGFR rabbit IgG(H+L) / donkey NGFR 162.38
Colo858-B2-rep1
B8
A-375 Vemurafenib UNC669 1.0000 0.5333
uM
 48
hr
 2 2288 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 162.78 NGFR rabbit IgG(H+L) / donkey NGFR 184.93
A375-B1-rep2
B8
WM-115 Vemurafenib UNC669 1.0000 0.5333
uM
 48
hr
 1 1278 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 156.58 NGFR rabbit IgG(H+L) / donkey NGFR 1084.81
WM115-B1-rep1
B8
COLO 858 Vemurafenib UNC669 0.3160 0.5333
uM
 48
hr
 1 1503 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 309.39 NGFR rabbit IgG(H+L) / donkey NGFR 152.71
Colo858-B1-rep1
B8
A-375 Vemurafenib UNC669 1.0000 0.1067
uM
 48
hr
 1 2260 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 166.44 NGFR rabbit IgG(H+L) / donkey NGFR 151.61
A375-B2-rep1
B8
A-375 Vemurafenib UNC669 1.0000 2.6667
uM
 48
hr
 1 2298 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 153.06 NGFR rabbit IgG(H+L) / donkey NGFR 186.36
A375-A2-rep1
B8
COLO 858 Vemurafenib UNC669 0.3160 2.6667
uM
 48
hr
 1 2097 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 357.07 NGFR rabbit IgG(H+L) / donkey NGFR 157.23
Colo858-A2-rep1
B8