Protein levels measured by imaging in BRAF(V600E/D) melanoma cell lines following treatment with Vemurafenib in combination with chromatin-targeting compounds (immunofluorescence dataset 3 of 4) - Dataset (ID:20276)

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Cell Small Molecule 1 Small Molecule 2 Small Mol 1 Concentration Small Mol 2 Concentration Small Mol Conc Unit Time Point Time Point Unit Replicate Number of Cells Primary Antibody (488) Secondary Antibody (488) Protein Detected (488) Mean Fluorescence Intensity (488) Primary Antibody (647) Secondary Antibody (647) Protein Detected (647) Mean Fluorescence Intensity (647) Plate Name Plate Well
A-375 Vemurafenib Olaparib 1.0000 2.6667
uM
 48
hr
 2 2229 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 159.21 NGFR rabbit IgG(H+L) / donkey NGFR 210.77
A375-A2-rep2
B2
A-375 Vemurafenib Olaparib 1.0000 0.5333
uM
 48
hr
 1 2160 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 155.98 NGFR rabbit IgG(H+L) / donkey NGFR 170.41
A375-B1-rep1
B2
COLO 858 Vemurafenib Mocetinostat 0.3160 2.6667
uM
 48
hr
 1 2109 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 155.83 NGFR rabbit IgG(H+L) / donkey NGFR 22.08
Colo858-A2-rep1
E4
COLO 858 Vemurafenib Mocetinostat 0.3160 2.6667
uM
 48
hr
 2 2331 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 165.13 NGFR rabbit IgG(H+L) / donkey NGFR 22.79
Colo858-A2-rep2
E4
A-375 Vemurafenib Mocetinostat 1.0000 0.5333
uM
 48
hr
 2 2584 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 288.63 NGFR rabbit IgG(H+L) / donkey NGFR 378.86
A375-B1-rep2
E4
A-375 Vemurafenib Mocetinostat 1.0000 0.1067
uM
 48
hr
 2 3376 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 267.71 NGFR rabbit IgG(H+L) / donkey NGFR 465.75
A375-B2-rep2
E4
COLO 858 Vemurafenib Mocetinostat 0.3160 0.1067
uM
 48
hr
 2 3092 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 219.79 NGFR rabbit IgG(H+L) / donkey NGFR 83.00
Colo858-B2-rep2
E4
COLO 858 Vemurafenib Mocetinostat 0.3160 0.5333
uM
 48
hr
 2 2847 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 153.05 NGFR rabbit IgG(H+L) / donkey NGFR 41.69
Colo858-B1-rep2
E4
WM-115 Vemurafenib Mocetinostat 1.0000 2.6667
uM
 48
hr
 2 1114 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 125.59 NGFR rabbit IgG(H+L) / donkey NGFR 597.14
WM115-A2-rep2
E4
A-375 Vemurafenib Mocetinostat 1.0000 2.6667
uM
 48
hr
 2 533 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 254.36 NGFR rabbit IgG(H+L) / donkey NGFR 81.09
A375-A2-rep2
E4
COLO 858 Vemurafenib Mocetinostat 0.3160 0.1067
uM
 48
hr
 1 2689 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 206.37 NGFR rabbit IgG(H+L) / donkey NGFR 98.91
Colo858-B2-rep1
E4
A-375 Vemurafenib Mocetinostat 1.0000 0.5333
uM
 48
hr
 1 2312 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 248.09 NGFR rabbit IgG(H+L) / donkey NGFR 431.39
A375-B1-rep1
E4
WM-115 Vemurafenib Mocetinostat 1.0000 0.1067
uM
 48
hr
 2 1572 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 120.44 NGFR rabbit IgG(H+L) / donkey NGFR 1493.64
WM115-B2-rep2
E4
WM-115 Vemurafenib Mocetinostat 1.0000 0.1067
uM
 48
hr
 1 1586 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 123.99 NGFR rabbit IgG(H+L) / donkey NGFR 1412.07
WM115-B2-rep1
E4
WM-115 Vemurafenib Mocetinostat 1.0000 0.5333
uM
 48
hr
 2 1590 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 119.27 NGFR rabbit IgG(H+L) / donkey NGFR 1037.35
WM115-B1-rep2
E4
COLO 858 Vemurafenib Mocetinostat 0.3160 0.5333
uM
 48
hr
 1 2873 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 157.87 NGFR rabbit IgG(H+L) / donkey NGFR 44.51
Colo858-B1-rep1
E4
WM-115 Vemurafenib Mocetinostat 1.0000 2.6667
uM
 48
hr
 1 987 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 136.34 NGFR rabbit IgG(H+L) / donkey NGFR 568.17
WM115-A2-rep1
E4
WM-115 Vemurafenib Mocetinostat 1.0000 0.5333
uM
 48
hr
 1 1518 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 134.70 NGFR rabbit IgG(H+L) / donkey NGFR 987.13
WM115-B1-rep1
E4
A-375 Vemurafenib Mocetinostat 1.0000 0.1067
uM
 48
hr
 1 2929 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 280.55 NGFR rabbit IgG(H+L) / donkey NGFR 517.04
A375-B2-rep1
E4
A-375 Vemurafenib Mocetinostat 1.0000 2.6667
uM
 48
hr
 1 304 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 299.57 NGFR rabbit IgG(H+L) / donkey NGFR 87.98
A375-A2-rep1
E4
COLO 858 Vemurafenib Methylstat 0.3160 2.6667
uM
 48
hr
 2 1461 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 193.86 NGFR rabbit IgG(H+L) / donkey NGFR 69.08
Colo858-A2-rep2
F6
COLO 858 Vemurafenib Methylstat 0.3160 0.1067
uM
 48
hr
 1 2248 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 279.63 NGFR rabbit IgG(H+L) / donkey NGFR 146.80
Colo858-B2-rep1
F6
A-375 Vemurafenib Methylstat 1.0000 0.1067
uM
 48
hr
 2 2638 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 143.17 NGFR rabbit IgG(H+L) / donkey NGFR 152.99
A375-B2-rep2
F6
COLO 858 Vemurafenib Methylstat 0.3160 0.1067
uM
 48
hr
 2 2596 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 315.46 NGFR rabbit IgG(H+L) / donkey NGFR 165.29
Colo858-B2-rep2
F6
COLO 858 Vemurafenib Methylstat 0.3160 0.5333
uM
 48
hr
 1 2664 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 266.70 NGFR rabbit IgG(H+L) / donkey NGFR 133.40
Colo858-B1-rep1
F6