Protein levels measured by imaging in BRAF(V600E/D) melanoma cell lines following treatment with Vemurafenib in combination with chromatin-targeting compounds (immunofluorescence dataset 3 of 4) - Dataset (ID:20276)

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Cell Small Molecule 1 Small Molecule 2 Small Mol 1 Concentration Small Mol 2 Concentration Small Mol Conc Unit Time Point Time Point Unit Replicate Number of Cells Primary Antibody (488) Secondary Antibody (488) Protein Detected (488) Mean Fluorescence Intensity (488) Primary Antibody (647) Secondary Antibody (647) Protein Detected (647) Mean Fluorescence Intensity (647) Plate Name Plate Well
A-375 Vemurafenib 1.0000
uM
 48
hr
 1 2252 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 227.51 NGFR rabbit IgG(H+L) / donkey NGFR 199.22
A375-A2-rep1
A5
A-375 Vemurafenib (+)-JQ1 1.0000 2.6667
uM
 48
hr
 2 2251 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 165.40 NGFR rabbit IgG(H+L) / donkey NGFR 86.81
A375-A2-rep2
C7
COLO 858 Vemurafenib Methylstat 0.3160 0.1067
uM
 48
hr
 1 2248 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 279.63 NGFR rabbit IgG(H+L) / donkey NGFR 146.80
Colo858-B2-rep1
F6
A-375 Vemurafenib 1.0000
uM
 48
hr
 1 2248 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 151.33 NGFR rabbit IgG(H+L) / donkey NGFR 175.00
A375-B1-rep1
A8
COLO 858 Vemurafenib C646 0.3160 0.1067
uM
 48
hr
 1 2247 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 313.23 NGFR rabbit IgG(H+L) / donkey NGFR 149.21
Colo858-B2-rep1
E3
COLO 858 Vemurafenib JW55 0.3160 2.6667
uM
 48
hr
 1 2243 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 243.79 NGFR rabbit IgG(H+L) / donkey NGFR 100.13
Colo858-A2-rep1
D3
A-375 Vemurafenib Rucaparib 1.0000 0.1067
uM
 48
hr
 2 2239 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 153.46 NGFR rabbit IgG(H+L) / donkey NGFR 159.08
A375-B2-rep2
C3
A-375 Vemurafenib 1.0000
uM
 48
hr
 1 2235 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 168.50 NGFR rabbit IgG(H+L) / donkey NGFR 181.65
A375-B1-rep1
A3
COLO 858 Vemurafenib Azacitidine 0.3160 0.1067
uM
 48
hr
 2 2234 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 274.09 NGFR rabbit IgG(H+L) / donkey NGFR 137.68
Colo858-B2-rep2
E2
A-375 Vemurafenib Olaparib 1.0000 2.6667
uM
 48
hr
 2 2229 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 159.21 NGFR rabbit IgG(H+L) / donkey NGFR 210.77
A375-A2-rep2
B2
A-375 Vemurafenib CTB 1.0000 0.1067
uM
 48
hr
 2 2227 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 169.07 NGFR rabbit IgG(H+L) / donkey NGFR 150.81
A375-B2-rep2
B4
COLO 858 Vemurafenib I-BET 0.3160 0.5333
uM
 48
hr
 2 2224 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 161.45 NGFR rabbit IgG(H+L) / donkey NGFR 24.73
Colo858-B1-rep2
E7
A-375 Vemurafenib CTB 1.0000 2.6667
uM
 48
hr
 1 2224 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 159.14 NGFR rabbit IgG(H+L) / donkey NGFR 160.82
A375-A2-rep1
B4
A-375 Vemurafenib Vorinostat 1.0000 2.6667
uM
 48
hr
 2 2222 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 252.58 NGFR rabbit IgG(H+L) / donkey NGFR 61.42
A375-A2-rep2
D2
A-375 Vemurafenib 1.0000
uM
 48
hr
 1 2222 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 152.21 NGFR rabbit IgG(H+L) / donkey NGFR 164.68
A375-B1-rep1
A9
A-375 Vemurafenib 1.0000
uM
 48
hr
 1 2214 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 229.79 NGFR rabbit IgG(H+L) / donkey NGFR 205.29
A375-A2-rep1
A2
A-375 Vemurafenib 1.0000
uM
 48
hr
 2 2208 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 193.23 NGFR rabbit IgG(H+L) / donkey NGFR 197.15
A375-B1-rep2
A4
A-375 Vemurafenib Veliparib 1.0000 0.5333
uM
 48
hr
 1 2207 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 158.60 NGFR rabbit IgG(H+L) / donkey NGFR 168.87
A375-B1-rep1
C2
A-375 Vemurafenib Azacitidine 1.0000 0.1067
uM
 48
hr
 2 2202 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 173.55 NGFR rabbit IgG(H+L) / donkey NGFR 137.51
A375-B2-rep2
E2
COLO 858 Vemurafenib Methylstat 0.3160 0.5333
uM
 48
hr
 2 2202 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 242.84 NGFR rabbit IgG(H+L) / donkey NGFR 132.23
Colo858-B1-rep2
F6
A-375 Vemurafenib Decitabine 1.0000 0.1067
uM
 48
hr
 1 2198 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 190.33 NGFR rabbit IgG(H+L) / donkey NGFR 172.41
A375-B2-rep1
F2
A-375 Vemurafenib PFI-1 1.0000 0.1067
uM
 48
hr
 2 2198 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 144.45 NGFR rabbit IgG(H+L) / donkey NGFR 167.49
A375-B2-rep2
B7
A-375 Vemurafenib 1.0000
uM
 48
hr
 2 2194 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 176.74 NGFR rabbit IgG(H+L) / donkey NGFR 173.12
A375-B1-rep2
A8
A-375 Vemurafenib 1.0000
uM
 48
hr
 2 2194 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 194.15 NGFR rabbit IgG(H+L) / donkey NGFR 186.08
A375-A2-rep2
A5
COLO 858 Vemurafenib Rucaparib 0.3160 2.6667
uM
 48
hr
 2 2191 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 274.88 NGFR rabbit IgG(H+L) / donkey NGFR 168.71
Colo858-A2-rep2
C3