Protein levels measured by imaging in BRAF(V600E/D) melanoma cell lines following treatment with Vemurafenib in combination with chromatin-targeting compounds (immunofluorescence dataset 3 of 4) - Dataset (ID:20276)

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Cell Small Molecule 1 Small Molecule 2 Small Mol 1 Concentration Small Mol 2 Concentration Small Mol Conc Unit Time Point Time Point Unit Replicate Number of Cells Primary Antibody (488) Secondary Antibody (488) Protein Detected (488) Mean Fluorescence Intensity (488) Primary Antibody (647) Secondary Antibody (647) Protein Detected (647) Mean Fluorescence Intensity (647) Plate Name Plate Well
WM-115 Vemurafenib UNC669 1.0000 0.1067
uM
 48
hr
 2 1142 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 138.65 NGFR rabbit IgG(H+L) / donkey NGFR 1180.01
WM115-B2-rep2
B8
WM-115 Vemurafenib IOX2 1.0000 0.1067
uM
 48
hr
 2 1530 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 136.30 NGFR rabbit IgG(H+L) / donkey NGFR 1179.71
WM115-B2-rep2
D8
WM-115 Vemurafenib 1.0000
uM
 48
hr
 2 1100 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 149.13 NGFR rabbit IgG(H+L) / donkey NGFR 1179.05
WM115-B2-rep2
A10
WM-115 Vemurafenib GSK-J2 1.0000 0.1067
uM
 48
hr
 1 1729 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 130.85 NGFR rabbit IgG(H+L) / donkey NGFR 1178.59
WM115-B2-rep1
C6
WM-115 Vemurafenib BIX-01294 1.0000 0.1067
uM
 48
hr
 2 1551 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 158.43 NGFR rabbit IgG(H+L) / donkey NGFR 1178.42
WM115-B2-rep2
F5
WM-115 Vemurafenib 1.0000
uM
 48
hr
 1 1383 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 149.01 NGFR rabbit IgG(H+L) / donkey NGFR 1177.58
WM115-B2-rep1
A6
WM-115 Vemurafenib AGK2 1.0000 0.5333
uM
 48
hr
 2 1023 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 128.12 NGFR rabbit IgG(H+L) / donkey NGFR 1175.15
WM115-B1-rep2
D5
WM-115 Vemurafenib Daminozide 1.0000 0.1067
uM
 48
hr
 2 1438 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 132.87 NGFR rabbit IgG(H+L) / donkey NGFR 1174.36
WM115-B2-rep2
E6
WM-115 Vemurafenib 1.0000
uM
 48
hr
 2 1179 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 152.88 NGFR rabbit IgG(H+L) / donkey NGFR 1171.65
WM115-B2-rep2
A11
WM-115 Vemurafenib GSK-J4 1.0000 0.5333
uM
 48
hr
 1 1681 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 133.56 NGFR rabbit IgG(H+L) / donkey NGFR 1168.38
WM115-B1-rep1
D6
WM-115 Vemurafenib 1.0000
uM
 48
hr
 1 1358 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 159.53 NGFR rabbit IgG(H+L) / donkey NGFR 1167.78
WM115-B2-rep1
A7
WM-115 Vemurafenib Tranylcypromine 1.0000 2.6667
uM
 48
hr
 2 906 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 122.81 NGFR rabbit IgG(H+L) / donkey NGFR 1167.67
WM115-A2-rep2
G6
WM-115 Vemurafenib 1.0000
uM
 48
hr
 2 1434 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 159.89 NGFR rabbit IgG(H+L) / donkey NGFR 1166.16
WM115-B2-rep2
A3
WM-115 Vemurafenib Daminozide 1.0000 0.5333
uM
 48
hr
 2 1419 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 134.10 NGFR rabbit IgG(H+L) / donkey NGFR 1163.35
WM115-B1-rep2
E6
WM-115 Vemurafenib Olaparib 1.0000 0.1067
uM
 48
hr
 2 1291 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 154.34 NGFR rabbit IgG(H+L) / donkey NGFR 1162.67
WM115-B2-rep2
B2
WM-115 Vemurafenib GSK-J1 1.0000 0.1067
uM
 48
hr
 1 1493 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 149.17 NGFR rabbit IgG(H+L) / donkey NGFR 1162.33
WM115-B2-rep1
B6
WM-115 Vemurafenib 1.0000
uM
 48
hr
 2 1108 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 148.07 NGFR rabbit IgG(H+L) / donkey NGFR 1162.21
WM115-B2-rep2
H2
WM-115 Vemurafenib 1.0000
uM
 48
hr
 2 956 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 128.27 NGFR rabbit IgG(H+L) / donkey NGFR 1161.55
WM115-B2-rep2
A8
WM-115 Vemurafenib Epigallocatechin gallate 1.0000 0.5333
uM
 48
hr
 2 1274 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 125.39 NGFR rabbit IgG(H+L) / donkey NGFR 1161.43
WM115-B1-rep2
E8
WM-115 Vemurafenib Epigallocatechin gallate 1.0000 0.1067
uM
 48
hr
 1 1508 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 142.32 NGFR rabbit IgG(H+L) / donkey NGFR 1161.16
WM115-B2-rep1
E8
WM-115 Vemurafenib Tranylcypromine 1.0000 0.5333
uM
 48
hr
 1 1076 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 134.75 NGFR rabbit IgG(H+L) / donkey NGFR 1159.45
WM115-B1-rep1
G6
WM-115 Vemurafenib Veliparib 1.0000 0.1067
uM
 48
hr
 1 1557 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 148.83 NGFR rabbit IgG(H+L) / donkey NGFR 1159.27
WM115-B2-rep1
C2
WM-115 Vemurafenib JW55 1.0000 0.1067
uM
 48
hr
 2 1482 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 140.32 NGFR rabbit IgG(H+L) / donkey NGFR 1155.40
WM115-B2-rep2
D3
WM-115 Vemurafenib Garcinol 1.0000 0.1067
uM
 48
hr
 1 1268 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 145.31 NGFR rabbit IgG(H+L) / donkey NGFR 1155.37
WM115-B2-rep1
F3
WM-115 Vemurafenib GSK-J1 1.0000 0.5333
uM
 48
hr
 1 1268 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 142.27 NGFR rabbit IgG(H+L) / donkey NGFR 1155.24
WM115-B1-rep1
B6