Protein levels measured by imaging in BRAF(V600E/D) melanoma cell lines following treatment with Vemurafenib in combination with chromatin-targeting compounds (immunofluorescence dataset 3 of 4) - Dataset (ID:20276)

 Full screen view
Filter results: >>>>
Cell Small Molecule 1 Small Molecule 2 Small Mol 1 Concentration Small Mol 2 Concentration Small Mol Conc Unit Time Point Time Point Unit Replicate Number of Cells Primary Antibody (488) Secondary Antibody (488) Protein Detected (488) Mean Fluorescence Intensity (488) Primary Antibody (647) Secondary Antibody (647) Protein Detected (647) Mean Fluorescence Intensity (647) Plate Name Plate Well
A-375 Vemurafenib Pracinostat 1.0000 2.6667
uM
 48
hr
 1 137 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 388.80 NGFR rabbit IgG(H+L) / donkey NGFR 39.73
A375-A2-rep1
F4
COLO 858 Vemurafenib (-)-JQ1 0.3160 0.1067
uM
 48
hr
 2 3368 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 384.19 NGFR rabbit IgG(H+L) / donkey NGFR 168.62
Colo858-B2-rep2
D7
COLO 858 Vemurafenib BIX-01294 0.3160 0.5333
uM
 48
hr
 1 2472 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 382.91 NGFR rabbit IgG(H+L) / donkey NGFR 124.85
Colo858-B1-rep1
F5
COLO 858 Vemurafenib 0.3160
uM
 48
hr
 1 1214 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 380.70 NGFR rabbit IgG(H+L) / donkey NGFR 139.87
Colo858-B1-rep1
A4
COLO 858 Vemurafenib 0.3160
uM
 48
hr
 1 1228 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 378.70 NGFR rabbit IgG(H+L) / donkey NGFR 125.18
Colo858-B1-rep1
A9
COLO 858 Vemurafenib C646 0.3160 0.1067
uM
 48
hr
 2 2646 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 378.38 NGFR rabbit IgG(H+L) / donkey NGFR 139.85
Colo858-B2-rep2
E3
COLO 858 Vemurafenib 0.3160
uM
 48
hr
 1 1149 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 377.09 NGFR rabbit IgG(H+L) / donkey NGFR 157.77
Colo858-B2-rep1
A10
COLO 858 Vemurafenib 0.3160
uM
 48
hr
 2 851 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 376.95 NGFR rabbit IgG(H+L) / donkey NGFR 149.87
Colo858-A2-rep2
A7
COLO 858 Vemurafenib 0.3160
uM
 48
hr
 1 970 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 376.63 NGFR rabbit IgG(H+L) / donkey NGFR 125.61
Colo858-B1-rep1
A8
COLO 858 Vemurafenib UNC1215 0.3160 2.6667
uM
 48
hr
 1 2700 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 375.51 NGFR rabbit IgG(H+L) / donkey NGFR 134.85
Colo858-A2-rep1
C8
COLO 858 Vemurafenib BIX-01294 0.3160 0.1067
uM
 48
hr
 1 2175 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 374.62 NGFR rabbit IgG(H+L) / donkey NGFR 150.11
Colo858-B2-rep1
F5
A-375 Vemurafenib Entinostat 1.0000 0.5333
uM
 48
hr
 1 3313 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 374.52 NGFR rabbit IgG(H+L) / donkey NGFR 366.22
A375-B1-rep1
D4
COLO 858 Vemurafenib 0.3160
uM
 48
hr
 1 1008 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 371.10 NGFR rabbit IgG(H+L) / donkey NGFR 120.59
Colo858-B1-rep1
A5
COLO 858 Vemurafenib 0.3160
uM
 48
hr
 2 1202 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 368.53 NGFR rabbit IgG(H+L) / donkey NGFR 150.06
Colo858-B2-rep2
A4
COLO 858 Vemurafenib GSK-J4 0.3160 0.1067
uM
 48
hr
 2 3387 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 368.22 NGFR rabbit IgG(H+L) / donkey NGFR 158.47
Colo858-B2-rep2
D6
COLO 858 Vemurafenib MC1568 0.3160 2.6667
uM
 48
hr
 1 1318 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 365.04 NGFR rabbit IgG(H+L) / donkey NGFR 114.40
Colo858-A2-rep1
G4
COLO 858 Vemurafenib 0.3160
uM
 48
hr
 1 964 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 364.09 NGFR rabbit IgG(H+L) / donkey NGFR 110.85
Colo858-A2-rep1
H2
COLO 858 Vemurafenib Garcinol 0.3160 0.1067
uM
 48
hr
 2 2520 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 363.75 NGFR rabbit IgG(H+L) / donkey NGFR 149.22
Colo858-B2-rep2
F3
COLO 858 Vemurafenib 0.3160
uM
 48
hr
 1 1184 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 361.53 NGFR rabbit IgG(H+L) / donkey NGFR 140.40
Colo858-B1-rep1
A2
A-375 Vemurafenib Belinostat 1.0000 2.6667
uM
 48
hr
 2 319 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 360.58 NGFR rabbit IgG(H+L) / donkey NGFR 26.94
A375-A2-rep2
C4
COLO 858 Vemurafenib 0.3160
uM
 48
hr
 2 1033 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 359.83 NGFR rabbit IgG(H+L) / donkey NGFR 196.89
Colo858-A2-rep2
A2
COLO 858 Vemurafenib Selisistat 0.3160 2.6667
uM
 48
hr
 1 3015 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 359.72 NGFR rabbit IgG(H+L) / donkey NGFR 105.69
Colo858-A2-rep1
C5
COLO 858 Vemurafenib BIX-01294 0.3160 0.5333
uM
 48
hr
 2 1928 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 358.98 NGFR rabbit IgG(H+L) / donkey NGFR 120.70
Colo858-B1-rep2
F5
COLO 858 Vemurafenib GSK-J2 0.3160 0.1067
uM
 48
hr
 2 2915 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 358.92 NGFR rabbit IgG(H+L) / donkey NGFR 159.25
Colo858-B2-rep2
C6
COLO 858 Vemurafenib JW55 0.3160 0.1067
uM
 48
hr
 2 2613 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 358.73 NGFR rabbit IgG(H+L) / donkey NGFR 151.57
Colo858-B2-rep2
D3