Protein levels measured by imaging in BRAF(V600E/D) melanoma cell lines following treatment with Vemurafenib in combination with chromatin-targeting compounds (immunofluorescence dataset 3 of 4) - Dataset (ID:20276)

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Cell Small Molecule 1 Small Molecule 2 Small Mol 1 Concentration Small Mol 2 Concentration Small Mol Conc Unit Time Point Time Point Unit Replicate Number of Cells Primary Antibody (488) Secondary Antibody (488) Protein Detected (488) Mean Fluorescence Intensity (488) Primary Antibody (647) Secondary Antibody (647) Protein Detected (647) Mean Fluorescence Intensity (647) Plate Name Plate Well
COLO 858 Vemurafenib Rocilinostat 0.3160 0.1067
uM
 48
hr
 2 2119 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 332.77 NGFR rabbit IgG(H+L) / donkey NGFR 180.07
Colo858-B2-rep2
B5
COLO 858 Vemurafenib GSK-J1 0.3160 0.1067
uM
 48
hr
 2 1949 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 408.18 NGFR rabbit IgG(H+L) / donkey NGFR 166.40
Colo858-B2-rep2
B6
COLO 858 Vemurafenib PFI-1 0.3160 0.1067
uM
 48
hr
 2 2090 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 400.42 NGFR rabbit IgG(H+L) / donkey NGFR 145.17
Colo858-B2-rep2
B7
COLO 858 Vemurafenib UNC669 0.3160 0.1067
uM
 48
hr
 2 1966 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 419.20 NGFR rabbit IgG(H+L) / donkey NGFR 177.46
Colo858-B2-rep2
B8
COLO 858 Vemurafenib Veliparib 0.3160 0.1067
uM
 48
hr
 2 2385 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 352.61 NGFR rabbit IgG(H+L) / donkey NGFR 183.60
Colo858-B2-rep2
C2
COLO 858 Vemurafenib Rucaparib 0.3160 0.1067
uM
 48
hr
 2 2779 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 314.05 NGFR rabbit IgG(H+L) / donkey NGFR 173.73
Colo858-B2-rep2
C3
COLO 858 Vemurafenib Belinostat 0.3160 0.1067
uM
 48
hr
 2 796 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 190.45 NGFR rabbit IgG(H+L) / donkey NGFR 129.48
Colo858-B2-rep2
C4
COLO 858 Vemurafenib Selisistat 0.3160 0.1067
uM
 48
hr
 2 2910 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 357.93 NGFR rabbit IgG(H+L) / donkey NGFR 146.71
Colo858-B2-rep2
C5
COLO 858 Vemurafenib GSK-J2 0.3160 0.1067
uM
 48
hr
 2 2915 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 358.92 NGFR rabbit IgG(H+L) / donkey NGFR 159.25
Colo858-B2-rep2
C6
COLO 858 Vemurafenib (+)-JQ1 0.3160 0.1067
uM
 48
hr
 2 2000 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 144.50 NGFR rabbit IgG(H+L) / donkey NGFR 27.98
Colo858-B2-rep2
C7
COLO 858 Vemurafenib UNC1215 0.3160 0.1067
uM
 48
hr
 2 2912 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 347.33 NGFR rabbit IgG(H+L) / donkey NGFR 170.30
Colo858-B2-rep2
C8
COLO 858 Vemurafenib Vorinostat 0.3160 0.1067
uM
 48
hr
 2 1954 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 264.42 NGFR rabbit IgG(H+L) / donkey NGFR 180.44
Colo858-B2-rep2
D2
COLO 858 Vemurafenib JW55 0.3160 0.1067
uM
 48
hr
 2 2613 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 358.73 NGFR rabbit IgG(H+L) / donkey NGFR 151.57
Colo858-B2-rep2
D3
COLO 858 Vemurafenib Entinostat 0.3160 0.1067
uM
 48
hr
 2 3440 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 282.63 NGFR rabbit IgG(H+L) / donkey NGFR 158.24
Colo858-B2-rep2
D4
COLO 858 Vemurafenib AGK2 0.3160 0.1067
uM
 48
hr
 2 2858 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 305.97 NGFR rabbit IgG(H+L) / donkey NGFR 137.70
Colo858-B2-rep2
D5
COLO 858 Vemurafenib GSK-J4 0.3160 0.1067
uM
 48
hr
 2 3387 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 368.22 NGFR rabbit IgG(H+L) / donkey NGFR 158.47
Colo858-B2-rep2
D6
COLO 858 Vemurafenib (-)-JQ1 0.3160 0.1067
uM
 48
hr
 2 3368 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 384.19 NGFR rabbit IgG(H+L) / donkey NGFR 168.62
Colo858-B2-rep2
D7
COLO 858 Vemurafenib IOX2 0.3160 0.1067
uM
 48
hr
 2 3195 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 403.34 NGFR rabbit IgG(H+L) / donkey NGFR 186.02
Colo858-B2-rep2
D8
COLO 858 Vemurafenib Azacitidine 0.3160 0.1067
uM
 48
hr
 2 2234 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 274.09 NGFR rabbit IgG(H+L) / donkey NGFR 137.68
Colo858-B2-rep2
E2
COLO 858 Vemurafenib C646 0.3160 0.1067
uM
 48
hr
 2 2646 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 378.38 NGFR rabbit IgG(H+L) / donkey NGFR 139.85
Colo858-B2-rep2
E3
COLO 858 Vemurafenib Mocetinostat 0.3160 0.1067
uM
 48
hr
 2 3092 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 219.79 NGFR rabbit IgG(H+L) / donkey NGFR 83.00
Colo858-B2-rep2
E4
COLO 858 Vemurafenib Resveratrol 0.3160 0.1067
uM
 48
hr
 2 3137 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 346.17 NGFR rabbit IgG(H+L) / donkey NGFR 157.63
Colo858-B2-rep2
E5
COLO 858 Vemurafenib Daminozide 0.3160 0.1067
uM
 48
hr
 2 3171 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 314.34 NGFR rabbit IgG(H+L) / donkey NGFR 161.15
Colo858-B2-rep2
E6
COLO 858 Vemurafenib I-BET 0.3160 0.1067
uM
 48
hr
 2 2556 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 250.08 NGFR rabbit IgG(H+L) / donkey NGFR 76.21
Colo858-B2-rep2
E7
COLO 858 Vemurafenib Epigallocatechin gallate 0.3160 0.1067
uM
 48
hr
 2 3297 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 322.94 NGFR rabbit IgG(H+L) / donkey NGFR 169.39
Colo858-B2-rep2
E8