Protein levels measured by imaging in BRAF(V600E/D) melanoma cell lines following treatment with Vemurafenib in combination with chromatin-targeting compounds (immunofluorescence dataset 3 of 4) - Dataset (ID:20276)

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Cell Small Molecule 1 Small Molecule 2 Small Mol 1 Concentration Small Mol 2 Concentration Small Mol Conc Unit Time Point Time Point Unit Replicate Number of Cells Primary Antibody (488) Secondary Antibody (488) Protein Detected (488) Mean Fluorescence Intensity (488) Primary Antibody (647) Secondary Antibody (647) Protein Detected (647) Mean Fluorescence Intensity (647) Plate Name Plate Well
A-375 Vemurafenib Veliparib 1.0000 0.5333
uM
 48
hr
 2 2518 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 186.55 NGFR rabbit IgG(H+L) / donkey NGFR 195.58
A375-B1-rep2
C2
A-375 Vemurafenib Rucaparib 1.0000 0.5333
uM
 48
hr
 2 2421 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 168.96 NGFR rabbit IgG(H+L) / donkey NGFR 176.80
A375-B1-rep2
C3
A-375 Vemurafenib Belinostat 1.0000 0.5333
uM
 48
hr
 2 2075 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 151.98 NGFR rabbit IgG(H+L) / donkey NGFR 20.46
A375-B1-rep2
C4
A-375 Vemurafenib Selisistat 1.0000 0.5333
uM
 48
hr
 2 2918 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 176.89 NGFR rabbit IgG(H+L) / donkey NGFR 184.29
A375-B1-rep2
C5
A-375 Vemurafenib GSK-J2 1.0000 0.5333
uM
 48
hr
 2 3124 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 156.22 NGFR rabbit IgG(H+L) / donkey NGFR 175.54
A375-B1-rep2
C6
A-375 Vemurafenib (+)-JQ1 1.0000 0.5333
uM
 48
hr
 2 2283 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 118.15 NGFR rabbit IgG(H+L) / donkey NGFR 74.90
A375-B1-rep2
C7
A-375 Vemurafenib UNC1215 1.0000 0.5333
uM
 48
hr
 2 2762 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 166.53 NGFR rabbit IgG(H+L) / donkey NGFR 192.69
A375-B1-rep2
C8
A-375 Vemurafenib Vorinostat 1.0000 0.5333
uM
 48
hr
 2 2869 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 443.59 NGFR rabbit IgG(H+L) / donkey NGFR 119.80
A375-B1-rep2
D2
A-375 Vemurafenib JW55 1.0000 0.5333
uM
 48
hr
 2 2687 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 161.93 NGFR rabbit IgG(H+L) / donkey NGFR 175.65
A375-B1-rep2
D3
A-375 Vemurafenib Entinostat 1.0000 0.5333
uM
 48
hr
 2 3686 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 391.97 NGFR rabbit IgG(H+L) / donkey NGFR 328.37
A375-B1-rep2
D4
A-375 Vemurafenib AGK2 1.0000 0.5333
uM
 48
hr
 2 1946 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 233.50 NGFR rabbit IgG(H+L) / donkey NGFR 153.50
A375-B1-rep2
D5
A-375 Vemurafenib GSK-J4 1.0000 0.5333
uM
 48
hr
 2 3446 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 193.35 NGFR rabbit IgG(H+L) / donkey NGFR 187.41
A375-B1-rep2
D6
A-375 Vemurafenib (-)-JQ1 1.0000 0.5333
uM
 48
hr
 2 3373 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 173.27 NGFR rabbit IgG(H+L) / donkey NGFR 195.44
A375-B1-rep2
D7
A-375 Vemurafenib IOX2 1.0000 0.5333
uM
 48
hr
 2 2889 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 180.12 NGFR rabbit IgG(H+L) / donkey NGFR 201.82
A375-B1-rep2
D8
A-375 Vemurafenib Azacitidine 1.0000 0.5333
uM
 48
hr
 2 2016 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 219.91 NGFR rabbit IgG(H+L) / donkey NGFR 107.93
A375-B1-rep2
E2
A-375 Vemurafenib C646 1.0000 0.5333
uM
 48
hr
 2 2684 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 170.48 NGFR rabbit IgG(H+L) / donkey NGFR 184.36
A375-B1-rep2
E3
A-375 Vemurafenib Mocetinostat 1.0000 0.5333
uM
 48
hr
 2 2584 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 288.63 NGFR rabbit IgG(H+L) / donkey NGFR 378.86
A375-B1-rep2
E4
A-375 Vemurafenib Resveratrol 1.0000 0.5333
uM
 48
hr
 2 2798 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 177.44 NGFR rabbit IgG(H+L) / donkey NGFR 162.07
A375-B1-rep2
E5
A-375 Vemurafenib Daminozide 1.0000 0.5333
uM
 48
hr
 2 3093 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 188.94 NGFR rabbit IgG(H+L) / donkey NGFR 179.22
A375-B1-rep2
E6
A-375 Vemurafenib I-BET 1.0000 0.5333
uM
 48
hr
 2 2710 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 145.52 NGFR rabbit IgG(H+L) / donkey NGFR 139.02
A375-B1-rep2
E7
A-375 Vemurafenib Epigallocatechin gallate 1.0000 0.5333
uM
 48
hr
 2 2866 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 196.38 NGFR rabbit IgG(H+L) / donkey NGFR 183.28
A375-B1-rep2
E8
A-375 Vemurafenib Decitabine 1.0000 0.5333
uM
 48
hr
 2 2061 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 195.89 NGFR rabbit IgG(H+L) / donkey NGFR 175.61
A375-B1-rep2
F2
A-375 Vemurafenib Garcinol 1.0000 0.5333
uM
 48
hr
 2 2319 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 196.18 NGFR rabbit IgG(H+L) / donkey NGFR 178.69
A375-B1-rep2
F3
A-375 Vemurafenib Pracinostat 1.0000 0.5333
uM
 48
hr
 2 2095 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 492.96 NGFR rabbit IgG(H+L) / donkey NGFR 61.23
A375-B1-rep2
F4
A-375 Vemurafenib BIX-01294 1.0000 0.5333
uM
 48
hr
 2 2173 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 148.24 NGFR rabbit IgG(H+L) / donkey NGFR 105.39
A375-B1-rep2
F5