Protein levels measured by imaging in BRAF(V600E/D) melanoma cell lines following treatment with Vemurafenib in combination with chromatin-targeting compounds (immunofluorescence dataset 3 of 4) - Dataset (ID:20276)

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Cell Small Molecule 1 Small Molecule 2 Small Mol 1 Concentration Small Mol 2 Concentration Small Mol Conc Unit Time Point Time Point Unit Replicate Number of Cells Primary Antibody (488) Secondary Antibody (488) Protein Detected (488) Mean Fluorescence Intensity (488) Primary Antibody (647) Secondary Antibody (647) Protein Detected (647) Mean Fluorescence Intensity (647) Plate Name Plate Well
A-375 Vemurafenib GSK-J4 1.0000 0.5333
uM
 48
hr
 1 3385 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 193.70 NGFR rabbit IgG(H+L) / donkey NGFR 193.11
A375-B1-rep1
D6
A-375 Vemurafenib (-)-JQ1 1.0000 0.5333
uM
 48
hr
 1 3215 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 162.83 NGFR rabbit IgG(H+L) / donkey NGFR 203.67
A375-B1-rep1
D7
A-375 Vemurafenib IOX2 1.0000 0.5333
uM
 48
hr
 1 2771 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 155.39 NGFR rabbit IgG(H+L) / donkey NGFR 201.39
A375-B1-rep1
D8
A-375 Vemurafenib Azacitidine 1.0000 0.5333
uM
 48
hr
 1 1856 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 191.48 NGFR rabbit IgG(H+L) / donkey NGFR 101.73
A375-B1-rep1
E2
A-375 Vemurafenib C646 1.0000 0.5333
uM
 48
hr
 1 2174 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 163.13 NGFR rabbit IgG(H+L) / donkey NGFR 179.34
A375-B1-rep1
E3
A-375 Vemurafenib Mocetinostat 1.0000 0.5333
uM
 48
hr
 1 2312 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 248.09 NGFR rabbit IgG(H+L) / donkey NGFR 431.39
A375-B1-rep1
E4
A-375 Vemurafenib Resveratrol 1.0000 0.5333
uM
 48
hr
 1 2611 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 166.44 NGFR rabbit IgG(H+L) / donkey NGFR 163.00
A375-B1-rep1
E5
A-375 Vemurafenib Daminozide 1.0000 0.5333
uM
 48
hr
 1 2948 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 168.03 NGFR rabbit IgG(H+L) / donkey NGFR 185.80
A375-B1-rep1
E6
A-375 Vemurafenib I-BET 1.0000 0.5333
uM
 48
hr
 1 2611 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 126.80 NGFR rabbit IgG(H+L) / donkey NGFR 177.94
A375-B1-rep1
E7
A-375 Vemurafenib Epigallocatechin gallate 1.0000 0.5333
uM
 48
hr
 1 2719 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 177.56 NGFR rabbit IgG(H+L) / donkey NGFR 199.89
A375-B1-rep1
E8
A-375 Vemurafenib Decitabine 1.0000 0.5333
uM
 48
hr
 1 1887 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 172.55 NGFR rabbit IgG(H+L) / donkey NGFR 167.68
A375-B1-rep1
F2
A-375 Vemurafenib Garcinol 1.0000 0.5333
uM
 48
hr
 1 2101 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 167.05 NGFR rabbit IgG(H+L) / donkey NGFR 180.94
A375-B1-rep1
F3
A-375 Vemurafenib Pracinostat 1.0000 0.5333
uM
 48
hr
 1 1766 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 425.92 NGFR rabbit IgG(H+L) / donkey NGFR 51.29
A375-B1-rep1
F4
A-375 Vemurafenib BIX-01294 1.0000 0.5333
uM
 48
hr
 1 1955 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 141.58 NGFR rabbit IgG(H+L) / donkey NGFR 118.50
A375-B1-rep1
F5
A-375 Vemurafenib Methylstat 1.0000 0.5333
uM
 48
hr
 1 2144 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 145.50 NGFR rabbit IgG(H+L) / donkey NGFR 118.86
A375-B1-rep1
F6
A-375 Vemurafenib I-BET151 1.0000 0.5333
uM
 48
hr
 1 2049 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 135.76 NGFR rabbit IgG(H+L) / donkey NGFR 89.42
A375-B1-rep1
F7
A-375 Vemurafenib CX-5461 1.0000 0.5333
uM
 48
hr
 1 1324 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 136.85 NGFR rabbit IgG(H+L) / donkey NGFR 142.81
A375-B1-rep1
F8
A-375 Vemurafenib RG-108 1.0000 0.5333
uM
 48
hr
 1 1759 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 168.66 NGFR rabbit IgG(H+L) / donkey NGFR 137.19
A375-B1-rep1
G2
A-375 Vemurafenib Anacardic acid 1.0000 0.5333
uM
 48
hr
 1 1888 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 172.64 NGFR rabbit IgG(H+L) / donkey NGFR 136.19
A375-B1-rep1
G3
A-375 Vemurafenib MC1568 1.0000 0.5333
uM
 48
hr
 1 1814 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 224.30 NGFR rabbit IgG(H+L) / donkey NGFR 186.50
A375-B1-rep1
G4
A-375 Vemurafenib UNC0638 1.0000 0.5333
uM
 48
hr
 1 1729 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 168.58 NGFR rabbit IgG(H+L) / donkey NGFR 130.93
A375-B1-rep1
G5
A-375 Vemurafenib Tranylcypromine 1.0000 0.5333
uM
 48
hr
 1 1525 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 168.96 NGFR rabbit IgG(H+L) / donkey NGFR 187.91
A375-B1-rep1
G6
A-375 Vemurafenib Ischemin 1.0000 0.5333
uM
 48
hr
 1 1542 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 160.46 NGFR rabbit IgG(H+L) / donkey NGFR 152.67
A375-B1-rep1
G7
A-375 Vemurafenib 1.0000
uM
 48
hr
 1 1276 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 179.66 NGFR rabbit IgG(H+L) / donkey NGFR 140.40
A375-B1-rep1
H10
A-375 Vemurafenib 1.0000
uM
 48
hr
 1 1345 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 189.98 NGFR rabbit IgG(H+L) / donkey NGFR 146.49
A375-B1-rep1
H11