Phosphorylation state and protein levels measured by imaging in BRAF(V600E/D) melanoma cell lines following treatment with combinations of two compounds (immunofluorescence dataset 1 of 4) - Dataset (ID:20274)

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Cell Small Molecule 1 Small Molecule 2 Small Mol 1 Concentration Small Mol 2 Concentration Small Mol Conc Unit Time Point Time Point Unit Replicate Primary Antibody (488) Secondary Antibody (488) Protein Detected (488) Mean Fluorescence Intensity (488) Primary Antibody (568) Secondary Antibody (568) Protein Detected (568) Mean Fluorescence Intensity (568) Primary Antibody (647) Secondary Antibody (647) Protein Detected (647) Mean Fluorescence Intensity (647) Plate Name Plate Well
COLO 858 Vemurafenib Palbociclib 0.316 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 -32.74 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 96.94 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 324.72
Colo858-P10
C10
COLO 858 Vemurafenib Palbociclib 0.316 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 -64.90 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 106.44 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 296.56
Colo858-P10
C11
COLO 858 Vemurafenib Saracatinib 0.316 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 660.16 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 204.73 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 340.10
Colo858-P10
C2
COLO 858 Vemurafenib Saracatinib 0.316 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 538.19 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 208.99 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 236.98
Colo858-P10
C3
COLO 858 Vemurafenib Pictilisib 0.316 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 475.95 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 189.54 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 276.54
Colo858-P10
C4
COLO 858 Vemurafenib Pictilisib 0.316 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 236.14 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 145.43 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 266.67
Colo858-P10
C5
COLO 858 Vemurafenib AZD8055 0.316 0.200
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 90.47 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 111.48 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 341.30
Colo858-P10
C6
COLO 858 Vemurafenib AZD8055 0.316 0.600
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 163.55 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 117.33 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 270.97
Colo858-P10
C7
COLO 858 Vemurafenib JNK-IN-8 0.316 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 379.77 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 163.52 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 71.75
Colo858-P10
C8
COLO 858 Vemurafenib JNK-IN-8 0.316 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 274.03 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 156.06 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 43.62
Colo858-P10
C9
COLO 858 Vemurafenib Palbociclib 1.000 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 69.97 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 110.48 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 396.58
Colo858-P10
D10
COLO 858 Vemurafenib Palbociclib 1.000 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 -5.82 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 126.17 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 354.21
Colo858-P10
D11
COLO 858 Vemurafenib Saracatinib 1.000 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 613.97 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 195.54 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 317.39
Colo858-P10
D2
COLO 858 Vemurafenib Saracatinib 1.000 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 515.09 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 183.61 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 182.81
Colo858-P10
D3
COLO 858 Vemurafenib Pictilisib 1.000 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 480.32 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 196.01 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 271.38
Colo858-P10
D4
COLO 858 Vemurafenib Pictilisib 1.000 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 146.50 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 149.92 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 238.64
Colo858-P10
D5
COLO 858 Vemurafenib AZD8055 1.000 0.200
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 74.58 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 137.33 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 387.96
Colo858-P10
D6
COLO 858 Vemurafenib AZD8055 1.000 0.600
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 72.49 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 109.75 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 309.34
Colo858-P10
D7
COLO 858 Vemurafenib JNK-IN-8 1.000 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 212.83 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 131.15 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 74.34
Colo858-P10
D8
COLO 858 Vemurafenib JNK-IN-8 1.000 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 150.00 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 141.64 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 45.05
Colo858-P10
D9
COLO 858 Vemurafenib Palbociclib 0.000 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 1123.26 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 197.24 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 99.59
Colo858-P10
E10
COLO 858 Vemurafenib Palbociclib 0.000 3.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 660.73 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 175.10 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 94.05
Colo858-P10
E11
COLO 858 Vemurafenib Saracatinib 0.000 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 2300.56 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 334.83 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 116.61
Colo858-P10
E2
COLO 858 Vemurafenib Saracatinib 0.000 3.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 2346.96 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 348.18 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 123.82
Colo858-P10
E3
COLO 858 Vemurafenib Pictilisib 0.000 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 1955.75 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 284.43 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 123.24
Colo858-P10
E4