Phosphorylation state and protein levels measured by imaging in BRAF(V600E/D) melanoma cell lines following treatment with combinations of two compounds (immunofluorescence dataset 1 of 4) - Dataset (ID:20274)

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Cell Small Molecule 1 Small Molecule 2 Small Mol 1 Concentration Small Mol 2 Concentration Small Mol Conc Unit Time Point Time Point Unit Replicate Primary Antibody (488) Secondary Antibody (488) Protein Detected (488) Mean Fluorescence Intensity (488) Primary Antibody (568) Secondary Antibody (568) Protein Detected (568) Mean Fluorescence Intensity (568) Primary Antibody (647) Secondary Antibody (647) Protein Detected (647) Mean Fluorescence Intensity (647) Plate Name Plate Well
A-375 Vemurafenib Pictilisib 0.000 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 1276.95 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 295.45 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 259.89
A375-P9
B5
A-375 Vemurafenib AZD8055 0.000 0.200
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 1456.45 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 303.11 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 331.28
A375-P9
B6
A-375 Vemurafenib AZD8055 0.000 0.600
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 1278.24 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 266.10 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 287.39
A375-P9
B7
A-375 Vemurafenib JNK-IN-8 0.000 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 2067.25 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 368.91 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 300.37
A375-P9
B8
A-375 Vemurafenib JNK-IN-8 0.000 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 1646.35 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 314.06 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 227.19
A375-P9
B9
A-375 Vemurafenib Palbociclib 0.316 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 292.25 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 111.45 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 355.00
A375-P9
C10
A-375 Vemurafenib Palbociclib 0.316 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 178.64 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 90.48 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 285.72
A375-P9
C11
A-375 Vemurafenib Saracatinib 0.316 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 536.72 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 150.82 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 375.04
A375-P9
C2
A-375 Vemurafenib Saracatinib 0.316 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 476.93 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 152.20 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 381.97
A375-P9
C3
A-375 Vemurafenib Pictilisib 0.316 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 673.91 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 162.97 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 443.49
A375-P9
C4
A-375 Vemurafenib Pictilisib 0.316 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 551.56 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 153.45 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 331.81
A375-P9
C5
A-375 Vemurafenib AZD8055 0.316 0.200
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 790.57 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 180.60 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 527.06
A375-P9
C6
A-375 Vemurafenib AZD8055 0.316 0.600
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 864.29 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 193.44 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 475.17
A375-P9
C7
A-375 Vemurafenib JNK-IN-8 0.316 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 437.81 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 154.75 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 403.71
A375-P9
C8
A-375 Vemurafenib JNK-IN-8 0.316 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 357.45 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 128.98 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 247.37
A375-P9
C9
A-375 Vemurafenib Palbociclib 1.000 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 255.93 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 106.20 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 332.86
A375-P9
D10
A-375 Vemurafenib Palbociclib 1.000 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 183.33 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 90.28 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 232.77
A375-P9
D11
A-375 Vemurafenib Saracatinib 1.000 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 494.11 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 146.18 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 368.49
A375-P9
D2
A-375 Vemurafenib Saracatinib 1.000 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 465.95 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 146.60 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 397.54
A375-P9
D3
A-375 Vemurafenib Pictilisib 1.000 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 532.41 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 156.22 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 408.72
A375-P9
D4
A-375 Vemurafenib Pictilisib 1.000 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 541.14 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 161.66 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 366.57
A375-P9
D5
A-375 Vemurafenib AZD8055 1.000 0.200
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 486.53 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 154.16 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 551.14
A375-P9
D6
A-375 Vemurafenib AZD8055 1.000 0.600
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 594.27 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 163.46 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 514.47
A375-P9
D7
A-375 Vemurafenib JNK-IN-8 1.000 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 244.73 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 108.68 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 392.33
A375-P9
D8
A-375 Vemurafenib JNK-IN-8 1.000 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 348.73 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 117.36 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 318.34
A375-P9
D9