Phosphorylation state and protein levels measured by imaging in BRAF(V600E/D) melanoma cell lines following treatment with combinations of two compounds (immunofluorescence dataset 1 of 4) - Dataset (ID:20274)

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Cell Small Molecule 1 Small Molecule 2 Small Mol 1 Concentration Small Mol 2 Concentration Small Mol Conc Unit Time Point Time Point Unit Replicate Primary Antibody (488) Secondary Antibody (488) Protein Detected (488) Mean Fluorescence Intensity (488) Primary Antibody (568) Secondary Antibody (568) Protein Detected (568) Mean Fluorescence Intensity (568) Primary Antibody (647) Secondary Antibody (647) Protein Detected (647) Mean Fluorescence Intensity (647) Plate Name Plate Well
A-375 Vemurafenib Dasatinib 0.000 1.000
uM
48
hr
1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 2722.08 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 510.86 NGFR rabbit IgG(H+L) / donkey NGFR 141.68
A375-P1
B10
A-375 Vemurafenib Dasatinib 0.000 3.000
uM
48
hr
1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 2000.92 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 389.89 NGFR rabbit IgG(H+L) / donkey NGFR 116.46
A375-P1
B11
A-375 Vemurafenib 0.000
uM
48
hr
1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 3094.28 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 552.89 NGFR rabbit IgG(H+L) / donkey NGFR 593.97
A375-P1
B2
A-375 Vemurafenib 0.000
uM
48
hr
1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 2981.15 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 513.45 NGFR rabbit IgG(H+L) / donkey NGFR 573.44
A375-P1
B3
A-375 Vemurafenib Trametinib 0.000 0.200
uM
48
hr
1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 521.55 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 174.42 NGFR rabbit IgG(H+L) / donkey NGFR 1215.18
A375-P1
B4
A-375 Vemurafenib Trametinib 0.000 0.600
uM
48
hr
1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 496.87 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 163.74 NGFR rabbit IgG(H+L) / donkey NGFR 1256.19
A375-P1
B5
A-375 Vemurafenib Defactinib 0.000 1.000
uM
48
hr
1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 2523.19 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 469.18 NGFR rabbit IgG(H+L) / donkey NGFR 305.98
A375-P1
B6
A-375 Vemurafenib Defactinib 0.000 3.000
uM
48
hr
1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 1985.46 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 359.03 NGFR rabbit IgG(H+L) / donkey NGFR 315.78
A375-P1
B7
A-375 Vemurafenib PF562271 0.000 1.000
uM
48
hr
1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 2497.37 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 448.69 NGFR rabbit IgG(H+L) / donkey NGFR 257.39
A375-P1
B8
A-375 Vemurafenib PF562271 0.000 3.000
uM
48
hr
1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 1339.47 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 253.18 NGFR rabbit IgG(H+L) / donkey NGFR 154.21
A375-P1
B9
A-375 Vemurafenib Dasatinib 0.316 1.000
uM
48
hr
1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 538.29 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 166.43 NGFR rabbit IgG(H+L) / donkey NGFR 167.43
A375-P1
C10
A-375 Vemurafenib Dasatinib 0.316 3.000
uM
48
hr
1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 559.44 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 178.24 NGFR rabbit IgG(H+L) / donkey NGFR 158.24
A375-P1
C11
A-375 Vemurafenib 0.316
uM
48
hr
1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 658.19 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 196.52 NGFR rabbit IgG(H+L) / donkey NGFR 784.50
A375-P1
C2
A-375 Vemurafenib 0.316
uM
48
hr
1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 786.50 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 210.29 NGFR rabbit IgG(H+L) / donkey NGFR 822.61
A375-P1
C3
A-375 Vemurafenib Trametinib 0.316 0.200
uM
48
hr
1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 475.69 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 152.94 NGFR rabbit IgG(H+L) / donkey NGFR 1151.49
A375-P1
C4
A-375 Vemurafenib Trametinib 0.316 0.600
uM
48
hr
1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 463.41 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 158.22 NGFR rabbit IgG(H+L) / donkey NGFR 1124.92
A375-P1
C5
A-375 Vemurafenib Defactinib 0.316 1.000
uM
48
hr
1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 621.35 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 166.51 NGFR rabbit IgG(H+L) / donkey NGFR 366.71
A375-P1
C6
A-375 Vemurafenib Defactinib 0.316 3.000
uM
48
hr
1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 476.63 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 143.31 NGFR rabbit IgG(H+L) / donkey NGFR 248.80
A375-P1
C7
A-375 Vemurafenib PF562271 0.316 1.000
uM
48
hr
1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 503.75 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 156.55 NGFR rabbit IgG(H+L) / donkey NGFR 566.55
A375-P1
C8
A-375 Vemurafenib PF562271 0.316 3.000
uM
48
hr
1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 516.73 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 165.19 NGFR rabbit IgG(H+L) / donkey NGFR 493.56
A375-P1
C9
A-375 Vemurafenib Dasatinib 1.000 1.000
uM
48
hr
1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 536.52 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 174.46 NGFR rabbit IgG(H+L) / donkey NGFR 300.18
A375-P1
D10
A-375 Vemurafenib Dasatinib 1.000 3.000
uM
48
hr
1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 540.13 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 175.10 NGFR rabbit IgG(H+L) / donkey NGFR 180.58
A375-P1
D11
A-375 Vemurafenib 1.000
uM
48
hr
1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 674.52 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 188.75 NGFR rabbit IgG(H+L) / donkey NGFR 856.39
A375-P1
D2
A-375 Vemurafenib 1.000
uM
48
hr
1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 661.81 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 185.37 NGFR rabbit IgG(H+L) / donkey NGFR 873.80
A375-P1
D3
A-375 Vemurafenib Trametinib 1.000 0.200
uM
48
hr
1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 550.54 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 169.58 NGFR rabbit IgG(H+L) / donkey NGFR 1008.81
A375-P1
D4
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