Phosphorylation state and protein levels measured by imaging in BRAF(V600E/D) melanoma cell lines following treatment with combinations of two compounds (immunofluorescence dataset 1 of 4) - Dataset (ID:20274)

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Cell Small Molecule 1 Small Molecule 2 Small Mol 1 Concentration Small Mol 2 Concentration Small Mol Conc Unit Time Point Time Point Unit Replicate Primary Antibody (488) Secondary Antibody (488) Protein Detected (488) Mean Fluorescence Intensity (488) Primary Antibody (568) Secondary Antibody (568) Protein Detected (568) Mean Fluorescence Intensity (568) Primary Antibody (647) Secondary Antibody (647) Protein Detected (647) Mean Fluorescence Intensity (647) Plate Name Plate Well
A-375 Vemurafenib Dasatinib 1.000 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 428.90 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 155.70 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 377.11
A375-P3
D11
A-375 Vemurafenib Dasatinib 0.000 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 2181.70 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 406.40 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 169.81
A375-P4
B10
A-375 Vemurafenib Dasatinib 1.000 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 468.48 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 159.69 NGFR rabbit IgG(H+L) / donkey NGFR 243.06
A375-P1
G10
COLO 858 Vemurafenib Dasatinib 1.000 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 631.13 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 190.51 NGFR rabbit IgG(H+L) / donkey NGFR 45.27
Colo858-P1
D11
COLO 858 Vemurafenib Dasatinib 0.000 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 3740.18 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 484.10 NGFR rabbit IgG(H+L) / donkey NGFR 116.54
Colo858-P1
E10
WM-115 Vemurafenib Dasatinib 0.316 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 521.99 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 144.36 NGFR rabbit IgG(H+L) / donkey NGFR 1045.50
WM115-P1
C10
A-375 Vemurafenib Dasatinib 1.000 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 540.13 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 175.10 NGFR rabbit IgG(H+L) / donkey NGFR 180.58
A375-P1
D11
COLO 858 Vemurafenib Dasatinib 0.316 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 718.46 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 187.45 NGFR rabbit IgG(H+L) / donkey NGFR 61.02
Colo858-P1
F10
COLO 858 Vemurafenib Dasatinib 0.000 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 3650.28 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 501.72 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 7537.77
Colo858-P2
E10
A-375 Vemurafenib Dasatinib 0.000 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 2483.07 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 422.64 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 223.37
A375-P3
E10
COLO 858 Vemurafenib Dasatinib 0.000 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 3684.23 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 505.69 NGFR rabbit IgG(H+L) / donkey NGFR 115.96
Colo858-P1
B10
A-375 Vemurafenib Dasatinib 0.000 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 2722.08 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 510.86 NGFR rabbit IgG(H+L) / donkey NGFR 141.68
A375-P1
B10
A-375 Vemurafenib Dasatinib 1.000 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 536.52 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 174.46 NGFR rabbit IgG(H+L) / donkey NGFR 300.18
A375-P1
D10
A-375 Vemurafenib Dasatinib 0.316 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 318.19 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 137.96 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 175.13
A375-P4
C11
A-375 Vemurafenib Dasatinib 1.000 3.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 418.26 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 153.76 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 706.45
A375-P2
G11
A-375 Vemurafenib Dasatinib 0.316 3.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 381.09 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 137.39 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 164.20
A375-P4
F11
A-375 Vemurafenib Dasatinib 1.000 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 451.00 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 158.28 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 691.02
A375-P2
G10
A-375 Vemurafenib Dasatinib 0.316 3.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 523.10 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 171.20 NGFR rabbit IgG(H+L) / donkey NGFR 138.23
A375-P1
F11
COLO 858 Vemurafenib Dasatinib 0.000 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 3387.57 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 414.22 NGFR rabbit IgG(H+L) / donkey NGFR 87.43
Colo858-P1
B11
COLO 858 Vemurafenib Dasatinib 1.000 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 526.85 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 175.22 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 615.97
Colo858-P2
D10
A-375 Vemurafenib Dasatinib 0.316 3.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 438.91 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 154.77 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 318.75
A375-P3
F11
A-375 Vemurafenib Dasatinib 0.316 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 550.44 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 170.79 NGFR rabbit IgG(H+L) / donkey NGFR 155.36
A375-P1
F10
COLO 858 Vemurafenib Dasatinib 0.316 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 610.87 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 183.14 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 1075.09
Colo858-P2
C11
A-375 Vemurafenib Dasatinib 0.316 3.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 502.70 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 172.60 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 1310.16
A375-P2
F11
COLO 858 Vemurafenib Dasatinib 1.000 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 640.45 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 179.90 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 721.38
Colo858-P2
D11