Phosphorylation state and protein levels measured by imaging in BRAF(V600E/D) melanoma cell lines following treatment with combinations of two compounds (immunofluorescence dataset 1 of 4) - Dataset (ID:20274)

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Cell Small Molecule 1 Small Molecule 2 Small Mol 1 Concentration Small Mol 2 Concentration Small Mol Conc Unit Time Point Time Point Unit Replicate Primary Antibody (488) Secondary Antibody (488) Protein Detected (488) Mean Fluorescence Intensity (488) Primary Antibody (568) Secondary Antibody (568) Protein Detected (568) Mean Fluorescence Intensity (568) Primary Antibody (647) Secondary Antibody (647) Protein Detected (647) Mean Fluorescence Intensity (647) Plate Name Plate Well
COLO 858 Vemurafenib PF562271 0.316 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 597.72 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 178.20 NGFR rabbit IgG(H+L) / donkey NGFR 290.02
Colo858-P1
C8
COLO 858 Vemurafenib PF562271 0.000 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 2298.64 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 314.68 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 5205.37
Colo858-P2
E8
WM-115 Vemurafenib PF562271 1.000 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 353.70 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 108.28 NGFR rabbit IgG(H+L) / donkey NGFR 2500.22
WM115-P1
D8
A-375 Vemurafenib PF562271 0.316 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 393.59 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 134.83 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 503.03
A375-P3
F8
WM-115 Vemurafenib PF562271 0.316 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 302.14 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 108.52 NGFR rabbit IgG(H+L) / donkey NGFR 1188.55
WM115-P1
C9
WM-115 Vemurafenib PF562271 0.000 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 227.39 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 122.23 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 85.18
WM115-P4
B9
WM-115 Vemurafenib PF562271 0.000 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 521.81 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 159.77 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 121.47
WM115-P4
B8
WM-115 Vemurafenib PF562271 1.000 3.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 320.65 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 105.65 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 173.92
WM115-P3
G9
WM-115 Vemurafenib PF562271 0.316 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 473.33 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 129.87 NGFR rabbit IgG(H+L) / donkey NGFR 1998.34
WM115-P1
C8
A-375 Vemurafenib PF562271 0.316 3.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 280.58 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 133.67 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 278.95
A375-P3
F9
COLO 858 Vemurafenib PF562271 0.000 3.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 1427.02 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 303.74 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 4955.35
Colo858-P2
E9
WM-115 Vemurafenib PF562271 1.000 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 337.71 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 105.57 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 1142.99
WM115-P2
D8
WM-115 Vemurafenib PF562271 0.000 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 400.82 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 163.70 NGFR rabbit IgG(H+L) / donkey NGFR 1013.88
WM115-P1
B9
A-375 Vemurafenib PF562271 0.316 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 251.99 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 136.92 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 225.32
A375-P4
C9
COLO 858 Vemurafenib PF562271 1.000 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 529.15 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 153.33 NGFR rabbit IgG(H+L) / donkey NGFR 402.57
Colo858-P1
G8
WM-115 Vemurafenib PF562271 0.000 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 768.10 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 194.55 NGFR rabbit IgG(H+L) / donkey NGFR 966.13
WM115-P1
B8
COLO 858 Vemurafenib PF562271 0.316 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 391.00 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 158.71 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 294.18
Colo858-P4
C8
COLO 858 Vemurafenib PF562271 0.316 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 326.90 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 155.90 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 250.06
Colo858-P4
C9
A-375 Vemurafenib PF562271 1.000 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 391.19 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 127.30 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 388.14
A375-P3
G8
A-375 Vemurafenib PF562271 1.000 3.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 350.60 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 131.47 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 244.17
A375-P3
G9
COLO 858 Vemurafenib PF562271 1.000 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 296.53 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 154.50 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 580.76
Colo858-P2
D8
WM-115 Vemurafenib PF562271 1.000 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 423.89 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 108.70 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 368.15
WM115-P3
G8
A-375 Vemurafenib PF562271 0.316 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 230.55 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 119.38 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 292.63
A375-P4
C8
WM-115 Vemurafenib PF562271 0.316 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 357.32 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 127.53 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 2700.27
WM115-P2
C9
WM-115 Vemurafenib PF562271 0.316 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 610.00 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 174.32 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 1784.41
WM115-P2
C8