Phosphorylation state and protein levels measured by imaging in BRAF(V600E/D) melanoma cell lines following treatment with combinations of two compounds (immunofluorescence dataset 1 of 4) - Dataset (ID:20274)

 Full screen view
Filter results: >>>>
Cell Small Molecule 1 Small Molecule 2 Small Mol 1 Concentration Small Mol 2 Concentration Small Mol Conc Unit Time Point Time Point Unit Replicate Primary Antibody (488) Secondary Antibody (488) Protein Detected (488) Mean Fluorescence Intensity (488) Primary Antibody (568) Secondary Antibody (568) Protein Detected (568) Mean Fluorescence Intensity (568) Primary Antibody (647) Secondary Antibody (647) Protein Detected (647) Mean Fluorescence Intensity (647) Plate Name Plate Well
A-375 Vemurafenib JNK-IN-8 1.000 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 441.74 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 139.78 NGFR rabbit IgG(H+L) / donkey NGFR 262.27
A375-P7
D9
A-375 Vemurafenib JNK-IN-8 1.000 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 345.98 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 120.79 NGFR rabbit IgG(H+L) / donkey NGFR 369.72
A375-P7
D8
COLO 858 Vemurafenib JNK-IN-8 0.316 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 408.59 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 173.38 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 602.34
Colo858-P8
C8
A-375 Vemurafenib JNK-IN-8 0.316 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 411.11 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 156.52 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 84.74
A375-P10
C9
A-375 Vemurafenib JNK-IN-8 0.316 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 477.45 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 147.57 NGFR rabbit IgG(H+L) / donkey NGFR 140.32
A375-P7
C9
A-375 Vemurafenib JNK-IN-8 0.316 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 413.43 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 139.34 NGFR rabbit IgG(H+L) / donkey NGFR 264.58
A375-P7
C8
A-375 Vemurafenib JNK-IN-8 0.316 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 413.18 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 160.47 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 115.79
A375-P10
C8
COLO 858 Vemurafenib JNK-IN-8 1.000 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 212.83 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 131.15 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 74.34
Colo858-P10
D8
A-375 Vemurafenib JNK-IN-8 0.000 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 2096.28 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 413.41 NGFR rabbit IgG(H+L) / donkey NGFR 143.89
A375-P7
B9
COLO 858 Vemurafenib JNK-IN-8 0.000 3.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 3190.16 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 401.49 NGFR rabbit IgG(H+L) / donkey NGFR 133.47
Colo858-P7
E9
A-375 Vemurafenib JNK-IN-8 0.000 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 2530.85 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 466.71 NGFR rabbit IgG(H+L) / donkey NGFR 357.38
A375-P7
B8
COLO 858 Vemurafenib JNK-IN-8 1.000 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 150.00 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 141.64 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 45.05
Colo858-P10
D9
COLO 858 Vemurafenib JNK-IN-8 0.000 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 2934.72 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 367.66 NGFR rabbit IgG(H+L) / donkey NGFR 174.84
Colo858-P7
E8
COLO 858 Vemurafenib JNK-IN-8 1.000 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 410.15 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 169.53 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 360.95
Colo858-P9
G8
COLO 858 Vemurafenib JNK-IN-8 1.000 3.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 392.22 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 165.29 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 238.96
Colo858-P9
G9
COLO 858 Vemurafenib JNK-IN-8 0.316 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 1147.74 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 196.08 NGFR rabbit IgG(H+L) / donkey NGFR 110.66
Colo858-P7
F8
COLO 858 Vemurafenib JNK-IN-8 0.316 3.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 538.05 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 185.81 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 204.99
Colo858-P9
F9
A-375 Vemurafenib JNK-IN-8 0.000 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 1645.39 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 328.55 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 123.98
A375-P10
B9
COLO 858 Vemurafenib JNK-IN-8 0.000 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 2327.77 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 344.76 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 109.17
Colo858-P10
B9
WM-115 Vemurafenib JNK-IN-8 0.000 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 906.98 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 259.11 NGFR rabbit IgG(H+L) / donkey NGFR 1044.06
WM115-P7
B9
WM-115 Vemurafenib JNK-IN-8 0.000 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 1071.42 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 277.53 NGFR rabbit IgG(H+L) / donkey NGFR 1202.27
WM115-P7
B8
COLO 858 Vemurafenib JNK-IN-8 1.000 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 1067.04 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 182.92 NGFR rabbit IgG(H+L) / donkey NGFR 254.47
Colo858-P7
D9
A-375 Vemurafenib JNK-IN-8 0.000 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 2008.37 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 365.17 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 143.22
A375-P10
B8
COLO 858 Vemurafenib JNK-IN-8 0.000 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 2139.92 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 327.50 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 99.24
Colo858-P10
B8
COLO 858 Vemurafenib JNK-IN-8 0.000 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 2263.15 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 326.36 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 180.07
Colo858-P9
E8