Phosphorylation state and protein levels measured by imaging in BRAF(V600E/D) melanoma cell lines following treatment with combinations of two compounds (immunofluorescence dataset 1 of 4) - Dataset (ID:20274)

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Cell Small Molecule 1 Small Molecule 2 Small Mol 1 Concentration Small Mol 2 Concentration Small Mol Conc Unit Time Point Time Point Unit Replicate Primary Antibody (488) Secondary Antibody (488) Protein Detected (488) Mean Fluorescence Intensity (488) Primary Antibody (568) Secondary Antibody (568) Protein Detected (568) Mean Fluorescence Intensity (568) Primary Antibody (647) Secondary Antibody (647) Protein Detected (647) Mean Fluorescence Intensity (647) Plate Name Plate Well
COLO 858 Vemurafenib JNK-IN-8 1.000 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 410.15 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 169.53 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 360.95
Colo858-P9
G8
COLO 858 Vemurafenib JNK-IN-8 0.000 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 2327.77 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 344.76 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 109.17
Colo858-P10
B9
A-375 Vemurafenib JNK-IN-8 0.316 3.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 563.89 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 174.96 NGFR rabbit IgG(H+L) / donkey NGFR 185.32
A375-P7
F9
A-375 Vemurafenib JNK-IN-8 0.316 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 439.99 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 126.76 NGFR rabbit IgG(H+L) / donkey NGFR 270.31
A375-P7
F8
COLO 858 Vemurafenib JNK-IN-8 0.000 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 2263.15 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 326.36 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 180.07
Colo858-P9
E8
COLO 858 Vemurafenib JNK-IN-8 0.000 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 2139.92 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 327.50 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 99.24
Colo858-P10
B8
A-375 Vemurafenib JNK-IN-8 0.000 3.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 2087.72 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 373.66 NGFR rabbit IgG(H+L) / donkey NGFR 130.03
A375-P7
E9
COLO 858 Vemurafenib JNK-IN-8 0.000 3.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 2609.00 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 359.04 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 207.86
Colo858-P9
E9
A-375 Vemurafenib JNK-IN-8 0.000 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 2413.32 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 408.16 NGFR rabbit IgG(H+L) / donkey NGFR 329.47
A375-P7
E8
COLO 858 Vemurafenib JNK-IN-8 0.316 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 1101.43 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 173.56 NGFR rabbit IgG(H+L) / donkey NGFR 152.71
Colo858-P7
C9
A-375 Vemurafenib JNK-IN-8 1.000 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 441.74 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 139.78 NGFR rabbit IgG(H+L) / donkey NGFR 262.27
A375-P7
D9
A-375 Vemurafenib JNK-IN-8 1.000 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 345.98 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 120.79 NGFR rabbit IgG(H+L) / donkey NGFR 369.72
A375-P7
D8
COLO 858 Vemurafenib JNK-IN-8 1.000 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 319.66 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 167.76 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 297.48
Colo858-P9
D9
COLO 858 Vemurafenib JNK-IN-8 0.316 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 1450.25 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 227.96 NGFR rabbit IgG(H+L) / donkey NGFR 177.02
Colo858-P7
C8
COLO 858 Vemurafenib JNK-IN-8 1.000 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 999.15 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 160.59 NGFR rabbit IgG(H+L) / donkey NGFR 195.26
Colo858-P7
D8
COLO 858 Vemurafenib JNK-IN-8 1.000 3.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 392.22 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 165.29 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 238.96
Colo858-P9
G9
A-375 Vemurafenib JNK-IN-8 0.316 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 411.11 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 156.52 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 84.74
A375-P10
C9
A-375 Vemurafenib JNK-IN-8 0.316 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 477.45 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 147.57 NGFR rabbit IgG(H+L) / donkey NGFR 140.32
A375-P7
C9
A-375 Vemurafenib JNK-IN-8 0.316 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 413.43 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 139.34 NGFR rabbit IgG(H+L) / donkey NGFR 264.58
A375-P7
C8
COLO 858 Vemurafenib JNK-IN-8 0.000 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 3107.60 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 379.72 NGFR rabbit IgG(H+L) / donkey NGFR 160.75
Colo858-P7
B8
A-375 Vemurafenib JNK-IN-8 0.316 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 413.18 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 160.47 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 115.79
A375-P10
C8
COLO 858 Vemurafenib JNK-IN-8 0.000 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 3266.62 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 411.78 NGFR rabbit IgG(H+L) / donkey NGFR 137.05
Colo858-P7
B9
COLO 858 Vemurafenib JNK-IN-8 1.000 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 300.94 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 147.84 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 85.73
Colo858-P10
G8
A-375 Vemurafenib JNK-IN-8 0.000 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 2096.28 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 413.41 NGFR rabbit IgG(H+L) / donkey NGFR 143.89
A375-P7
B9
A-375 Vemurafenib JNK-IN-8 0.000 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 2530.85 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 466.71 NGFR rabbit IgG(H+L) / donkey NGFR 357.38
A375-P7
B8