Phosphorylation state and protein levels measured by imaging in BRAF(V600E/D) melanoma cell lines following treatment with combinations of two compounds (immunofluorescence dataset 1 of 4) - Dataset (ID:20274)

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Cell Small Molecule 1 Small Molecule 2 Small Mol 1 Concentration Small Mol 2 Concentration Small Mol Conc Unit Time Point Time Point Unit Replicate Primary Antibody (488) Secondary Antibody (488) Protein Detected (488) Mean Fluorescence Intensity (488) Primary Antibody (568) Secondary Antibody (568) Protein Detected (568) Mean Fluorescence Intensity (568) Primary Antibody (647) Secondary Antibody (647) Protein Detected (647) Mean Fluorescence Intensity (647) Plate Name Plate Well
WM-115 Vemurafenib Dasatinib 0.316 3.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 480.31 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 139.83 NGFR rabbit IgG(H+L) / donkey NGFR 889.64
WM115-P1
F11
WM-115 Vemurafenib Dasatinib 1.000 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 460.58 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 134.16 NGFR rabbit IgG(H+L) / donkey NGFR 1331.67
WM115-P1
G10
COLO 858 Vemurafenib Dasatinib 0.000 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 3881.18 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 466.69 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 124.41
Colo858-P4
B10
COLO 858 Vemurafenib Dasatinib 0.316 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 555.02 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 174.48 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 110.85
Colo858-P4
C10
A-375 Vemurafenib Dasatinib 0.000 3.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 1602.55 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 398.75 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 8219.91
A375-P2
E11
COLO 858 Vemurafenib Dasatinib 0.000 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 3490.89 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 412.36 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 99.67
Colo858-P4
B11
WM-115 Vemurafenib Dasatinib 0.316 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 540.70 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 143.61 NGFR rabbit IgG(H+L) / donkey NGFR 908.83
WM115-P1
F10
A-375 Vemurafenib Dasatinib 0.000 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 2344.02 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 501.25 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 9278.64
A375-P2
E10
COLO 858 Vemurafenib Dasatinib 1.000 3.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 499.31 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 227.65 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 121.00
Colo858-P3
G11
WM-115 Vemurafenib Dasatinib 1.000 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 523.31 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 146.67 NGFR rabbit IgG(H+L) / donkey NGFR 1460.75
WM115-P1
D10
WM-115 Vemurafenib Dasatinib 1.000 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 466.73 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 136.01 NGFR rabbit IgG(H+L) / donkey NGFR 1258.98
WM115-P1
D11
WM-115 Vemurafenib Dasatinib 0.000 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 805.51 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 205.67 NGFR rabbit IgG(H+L) / donkey NGFR 546.41
WM115-P1
E10
COLO 858 Vemurafenib Dasatinib 0.316 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 971.55 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 199.36 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 94.58
Colo858-P4
C11
COLO 858 Vemurafenib Dasatinib 0.000 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 3684.23 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 505.69 NGFR rabbit IgG(H+L) / donkey NGFR 115.96
Colo858-P1
B10
A-375 Vemurafenib Dasatinib 0.316 3.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 381.09 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 137.39 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 164.20
A375-P4
F11
WM-115 Vemurafenib Dasatinib 0.316 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 438.37 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 136.41 NGFR rabbit IgG(H+L) / donkey NGFR 1154.86
WM115-P1
C11
WM-115 Vemurafenib Dasatinib 0.000 3.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 641.16 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 176.08 NGFR rabbit IgG(H+L) / donkey NGFR 547.42
WM115-P1
E11
A-375 Vemurafenib Dasatinib 1.000 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 499.71 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 175.91 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 1034.98
A375-P2
D11
COLO 858 Vemurafenib Dasatinib 0.316 3.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 733.06 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 204.44 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 138.01
Colo858-P3
F11
A-375 Vemurafenib Dasatinib 1.000 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 460.82 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 157.29 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 607.39
A375-P2
D10
COLO 858 Vemurafenib Dasatinib 0.316 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 509.69 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 173.36 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 192.18
Colo858-P3
F10
COLO 858 Vemurafenib Dasatinib 0.000 3.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 3639.67 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 456.21 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 112.58
Colo858-P3
E11
WM-115 Vemurafenib Dasatinib 0.316 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 521.99 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 144.36 NGFR rabbit IgG(H+L) / donkey NGFR 1045.50
WM115-P1
C10
WM-115 Vemurafenib Dasatinib 0.000 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 554.72 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 184.01 NGFR rabbit IgG(H+L) / donkey NGFR 580.80
WM115-P1
B11
WM-115 Vemurafenib Dasatinib 0.000 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 734.28 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 203.21 NGFR rabbit IgG(H+L) / donkey NGFR 624.02
WM115-P1
B10