Phosphorylation state and protein levels measured by imaging in BRAF(V600E/D) melanoma cell lines following treatment with combinations of two compounds (immunofluorescence dataset 1 of 4) - Dataset (ID:20274)

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Cell Small Molecule 1 Small Molecule 2 Small Mol 1 Concentration Small Mol 2 Concentration Small Mol Conc Unit Time Point Time Point Unit Replicate Primary Antibody (488) Secondary Antibody (488) Protein Detected (488) Mean Fluorescence Intensity (488) Primary Antibody (568) Secondary Antibody (568) Protein Detected (568) Mean Fluorescence Intensity (568) Primary Antibody (647) Secondary Antibody (647) Protein Detected (647) Mean Fluorescence Intensity (647) Plate Name Plate Well
A-375 Vemurafenib Pictilisib 0.000 3.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 1133.62 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 249.77 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 233.49
A375-P9
E5
A-375 Vemurafenib AZD8055 0.000 0.200
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 1296.78 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 279.58 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 298.21
A375-P9
E6
A-375 Vemurafenib AZD8055 0.000 0.600
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 1304.14 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 256.23 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 279.80
A375-P9
E7
A-375 Vemurafenib JNK-IN-8 0.000 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 2058.90 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 364.89 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 252.32
A375-P9
E8
A-375 Vemurafenib JNK-IN-8 0.000 3.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 1736.29 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 310.44 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 203.47
A375-P9
E9
A-375 Vemurafenib Palbociclib 0.316 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 238.66 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 92.75 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 330.32
A375-P9
F10
A-375 Vemurafenib Palbociclib 0.316 3.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 182.69 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 86.52 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 266.67
A375-P9
F11
A-375 Vemurafenib Saracatinib 0.316 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 464.35 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 135.24 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 330.83
A375-P9
F2
A-375 Vemurafenib Saracatinib 0.316 3.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 540.45 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 153.39 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 337.65
A375-P9
F3
A-375 Vemurafenib Pictilisib 0.316 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 760.67 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 203.41 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 473.87
A375-P9
F4
A-375 Vemurafenib Pictilisib 0.316 3.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 602.27 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 170.12 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 361.62
A375-P9
F5
A-375 Vemurafenib AZD8055 0.316 0.200
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 797.38 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 184.78 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 473.97
A375-P9
F6
A-375 Vemurafenib AZD8055 0.316 0.600
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 940.86 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 181.09 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 405.15
A375-P9
F7
A-375 Vemurafenib JNK-IN-8 0.316 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 384.74 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 127.81 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 332.07
A375-P9
F8
A-375 Vemurafenib JNK-IN-8 0.316 3.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 411.56 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 120.50 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 209.21
A375-P9
F9
A-375 Vemurafenib Palbociclib 1.000 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 232.73 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 98.23 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 303.24
A375-P9
G10
A-375 Vemurafenib Palbociclib 1.000 3.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 203.98 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 101.43 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 264.85
A375-P9
G11
A-375 Vemurafenib Saracatinib 1.000 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 426.53 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 126.28 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 299.21
A375-P9
G2
A-375 Vemurafenib Saracatinib 1.000 3.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 473.40 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 139.53 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 307.92
A375-P9
G3
A-375 Vemurafenib Pictilisib 1.000 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 610.64 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 188.49 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 447.56
A375-P9
G4
A-375 Vemurafenib Pictilisib 1.000 3.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 589.31 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 160.08 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 343.41
A375-P9
G5
A-375 Vemurafenib AZD8055 1.000 0.200
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 709.09 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 175.17 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 490.12
A375-P9
G6
A-375 Vemurafenib AZD8055 1.000 0.600
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 672.99 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 167.19 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 410.14
A375-P9
G7
A-375 Vemurafenib JNK-IN-8 1.000 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 326.16 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 102.57 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 297.95
A375-P9
G8
A-375 Vemurafenib JNK-IN-8 1.000 3.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 379.81 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 117.13 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 256.19
A375-P9
G9