Phosphorylation state and protein levels measured by imaging in BRAF(V600E/D) melanoma cell lines following treatment with combinations of two compounds (immunofluorescence dataset 1 of 4) - Dataset (ID:20274)

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Cell Small Molecule 1 Small Molecule 2 Small Mol 1 Concentration Small Mol 2 Concentration Small Mol Conc Unit Time Point Time Point Unit Replicate Primary Antibody (488) Secondary Antibody (488) Protein Detected (488) Mean Fluorescence Intensity (488) Primary Antibody (568) Secondary Antibody (568) Protein Detected (568) Mean Fluorescence Intensity (568) Primary Antibody (647) Secondary Antibody (647) Protein Detected (647) Mean Fluorescence Intensity (647) Plate Name Plate Well
COLO 858 Vemurafenib Dasatinib 1.000 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 631.13 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 190.51 NGFR rabbit IgG(H+L) / donkey NGFR 45.27
Colo858-P1
D11
COLO 858 Vemurafenib 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 957.68 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 217.74 NGFR rabbit IgG(H+L) / donkey NGFR 769.46
Colo858-P1
D2
COLO 858 Vemurafenib 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 807.64 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 196.69 NGFR rabbit IgG(H+L) / donkey NGFR 771.35
Colo858-P1
D3
COLO 858 Vemurafenib Trametinib 1.000 0.200
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 582.43 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 155.15 NGFR rabbit IgG(H+L) / donkey NGFR 1119.00
Colo858-P1
D4
COLO 858 Vemurafenib Trametinib 1.000 0.600
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 509.49 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 177.92 NGFR rabbit IgG(H+L) / donkey NGFR 1008.58
Colo858-P1
D5
COLO 858 Vemurafenib Defactinib 1.000 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 338.46 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 172.71 NGFR rabbit IgG(H+L) / donkey NGFR 272.57
Colo858-P1
D6
COLO 858 Vemurafenib Defactinib 1.000 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 303.50 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 112.14 NGFR rabbit IgG(H+L) / donkey NGFR 133.45
Colo858-P1
D7
COLO 858 Vemurafenib PF562271 1.000 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 401.40 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 139.60 NGFR rabbit IgG(H+L) / donkey NGFR 422.47
Colo858-P1
D8
COLO 858 Vemurafenib PF562271 1.000 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 491.86 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 154.52 NGFR rabbit IgG(H+L) / donkey NGFR 126.90
Colo858-P1
D9
A-375 Vemurafenib Trametinib 0.316 0.200
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 232.02 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 123.94 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 304.95
A375-P4
C4
A-375 Vemurafenib Trametinib 0.316 0.600
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 182.46 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 119.76 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 395.54
A375-P4
C5
A-375 Vemurafenib Defactinib 0.000 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 1907.41 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 403.84 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 7872.32
A375-P2
B7
A-375 Vemurafenib Defactinib 0.316 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 264.22 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 130.48 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 290.31
A375-P4
C6
A-375 Vemurafenib Trametinib 0.316 0.600
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 463.41 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 158.22 NGFR rabbit IgG(H+L) / donkey NGFR 1124.92
A375-P1
C5
A-375 Vemurafenib Defactinib 0.316 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 291.00 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 125.75 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 263.71
A375-P4
C7
A-375 Vemurafenib PF562271 0.000 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 2470.37 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 465.05 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 7713.06
A375-P2
B8
A-375 Vemurafenib PF562271 0.316 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 230.55 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 119.38 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 292.63
A375-P4
C8
COLO 858 Vemurafenib Saracatinib 0.000 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 2470.40 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 370.68 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 125.42
Colo858-P10
B3
A-375 Vemurafenib PF562271 0.316 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 251.99 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 136.92 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 225.32
A375-P4
C9
A-375 Vemurafenib PF562271 0.000 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 1538.44 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 307.16 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 7914.56
A375-P2
B9
A-375 Vemurafenib Dasatinib 1.000 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 255.46 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 125.55 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 223.89
A375-P4
D10
COLO 858 Vemurafenib AZD8055 0.000 0.600
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 843.11 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 169.92 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 82.52
Colo858-P10
B7
A-375 Vemurafenib Dasatinib 1.000 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 381.61 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 152.57 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 194.41
A375-P4
D11
A-375 Vemurafenib Dasatinib 0.316 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 476.42 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 172.28 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 1134.30
A375-P2
C10
WM-115 Vemurafenib Pictilisib 0.316 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 427.24 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 110.14 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 191.66
WM115-P10
C4