Phosphorylation state and protein levels measured by imaging in BRAF(V600E/D) melanoma cell lines following treatment with combinations of two compounds (immunofluorescence dataset 1 of 4) - Dataset (ID:20274)

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Cell Small Molecule 1 Small Molecule 2 Small Mol 1 Concentration Small Mol 2 Concentration Small Mol Conc Unit Time Point Time Point Unit Replicate Primary Antibody (488) Secondary Antibody (488) Protein Detected (488) Mean Fluorescence Intensity (488) Primary Antibody (568) Secondary Antibody (568) Protein Detected (568) Mean Fluorescence Intensity (568) Primary Antibody (647) Secondary Antibody (647) Protein Detected (647) Mean Fluorescence Intensity (647) Plate Name Plate Well
A-375 Vemurafenib JNK-IN-8 0.000 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 2008.37 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 365.17 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 143.22
A375-P10
B8
A-375 Vemurafenib PF562271 0.316 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 444.44 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 148.39 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 1227.74
A375-P2
C8
A-375 Vemurafenib Pictilisib 0.000 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 1903.13 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 389.14 NGFR rabbit IgG(H+L) / donkey NGFR 273.51
A375-P7
B5
A-375 Vemurafenib PF562271 0.316 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 412.38 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 150.32 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 413.56
A375-P2
C9
A-375 Vemurafenib JNK-IN-8 0.000 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 1645.39 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 328.55 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 123.98
A375-P10
B9
A-375 Vemurafenib Dasatinib 1.000 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 460.82 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 157.29 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 607.39
A375-P2
D10
A-375 Vemurafenib JNK-IN-8 0.000 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 2096.28 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 413.41 NGFR rabbit IgG(H+L) / donkey NGFR 143.89
A375-P7
B9
A-375 Vemurafenib Dasatinib 1.000 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 499.71 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 175.91 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 1034.98
A375-P2
D11
A-375 Vemurafenib Palbociclib 0.316 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 296.34 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 131.61 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 266.72
A375-P10
C10
A-375 Vemurafenib 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 725.92 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 207.31 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 1613.01
A375-P2
D2
A-375 Vemurafenib Saracatinib 0.316 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 630.31 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 169.48 NGFR rabbit IgG(H+L) / donkey NGFR 213.50
A375-P7
C3
A-375 Vemurafenib 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 605.18 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 171.95 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 1664.46
A375-P2
D3
A-375 Vemurafenib Palbociclib 0.316 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 118.73 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 92.50 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 196.46
A375-P10
C11
A-375 Vemurafenib Trametinib 1.000 0.200
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 434.07 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 144.84 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 1630.59
A375-P2
D4
A-375 Vemurafenib JNK-IN-8 0.316 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 477.45 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 147.57 NGFR rabbit IgG(H+L) / donkey NGFR 140.32
A375-P7
C9
A-375 Vemurafenib Trametinib 1.000 0.600
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 324.90 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 136.79 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 2172.61
A375-P2
D5
A-375 Vemurafenib Saracatinib 0.316 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 553.91 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 156.05 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 233.07
A375-P10
C2
A-375 Vemurafenib Defactinib 1.000 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 275.62 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 127.43 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 741.69
A375-P2
D6
A-375 Vemurafenib Saracatinib 1.000 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 614.51 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 174.13 NGFR rabbit IgG(H+L) / donkey NGFR 225.89
A375-P7
D3
A-375 Vemurafenib Defactinib 1.000 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 284.43 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 121.47 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 393.84
A375-P2
D7
A-375 Vemurafenib Saracatinib 0.316 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 465.64 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 166.19 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 247.07
A375-P10
C3
A-375 Vemurafenib PF562271 1.000 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 423.13 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 151.80 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 703.18
A375-P2
D8
A-375 Vemurafenib JNK-IN-8 1.000 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 345.98 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 120.79 NGFR rabbit IgG(H+L) / donkey NGFR 369.72
A375-P7
D8
A-375 Vemurafenib PF562271 1.000 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 436.36 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 141.72 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 437.53
A375-P2
D9
COLO 858 Vemurafenib JNK-IN-8 1.000 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 150.00 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 141.64 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 45.05
Colo858-P10
D9