Phosphorylation state and protein levels measured by imaging in BRAF(V600E/D) melanoma cell lines following treatment with combinations of two compounds (immunofluorescence dataset 1 of 4) - Dataset (ID:20274)

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Cell Small Molecule 1 Small Molecule 2 Small Mol 1 Concentration Small Mol 2 Concentration Small Mol Conc Unit Time Point Time Point Unit Replicate Primary Antibody (488) Secondary Antibody (488) Protein Detected (488) Mean Fluorescence Intensity (488) Primary Antibody (568) Secondary Antibody (568) Protein Detected (568) Mean Fluorescence Intensity (568) Primary Antibody (647) Secondary Antibody (647) Protein Detected (647) Mean Fluorescence Intensity (647) Plate Name Plate Well
COLO 858 Vemurafenib Dasatinib 1.000 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 442.01 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 173.57 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 141.54
Colo858-P3
D11
A-375 Vemurafenib Saracatinib 0.316 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 536.72 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 150.82 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 375.04
A375-P9
C2
COLO 858 Vemurafenib 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 622.44 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 170.39 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 761.59
Colo858-P3
D3
A-375 Vemurafenib AZD8055 0.316 0.200
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 790.57 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 180.60 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 527.06
A375-P9
C6
A-375 Vemurafenib JNK-IN-8 0.316 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 357.45 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 128.98 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 247.37
A375-P9
C9
A-375 Vemurafenib Pictilisib 0.316 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 551.56 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 153.45 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 331.81
A375-P9
C5
COLO 858 Vemurafenib PF562271 1.000 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 345.37 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 149.25 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 684.43
Colo858-P3
D8
A-375 Vemurafenib JNK-IN-8 0.316 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 437.81 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 154.75 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 403.71
A375-P9
C8
COLO 858 Vemurafenib Dasatinib 0.000 3.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 3639.67 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 456.21 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 112.58
Colo858-P3
E11
A-375 Vemurafenib AZD8055 0.316 0.600
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 864.29 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 193.44 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 475.17
A375-P9
C7
COLO 858 Vemurafenib 0.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 2543.56 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 325.24 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 151.83
Colo858-P3
E3
A-375 Vemurafenib Saracatinib 1.000 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 465.95 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 146.60 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 397.54
A375-P9
D3
A-375 Vemurafenib AZD8055 1.000 0.200
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 486.53 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 154.16 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 551.14
A375-P9
D6
A-375 Vemurafenib Saracatinib 1.000 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 494.11 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 146.18 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 368.49
A375-P9
D2
A-375 Vemurafenib Pictilisib 0.316 3.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 602.27 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 170.12 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 361.62
A375-P9
F5
A-375 Vemurafenib Pictilisib 1.000 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 541.14 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 161.66 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 366.57
A375-P9
D5
COLO 858 Vemurafenib Dasatinib 0.316 3.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 733.06 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 204.44 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 138.01
Colo858-P3
F11
A-375 Vemurafenib Pictilisib 1.000 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 532.41 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 156.22 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 408.72
A375-P9
D4
COLO 858 Vemurafenib 0.316
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 986.51 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 219.91 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 678.93
Colo858-P3
F3
A-375 Vemurafenib JNK-IN-8 1.000 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 244.73 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 108.68 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 392.33
A375-P9
D8
A-375 Vemurafenib Saracatinib 0.000 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 2149.26 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 379.30 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 222.58
A375-P9
E2
A-375 Vemurafenib AZD8055 1.000 0.600
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 594.27 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 163.46 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 514.47
A375-P9
D7
COLO 858 Vemurafenib PF562271 0.316 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 391.70 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 155.91 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 642.08
Colo858-P3
F8
WM-115 Vemurafenib PF562271 1.000 3.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 320.65 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 105.65 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 173.92
WM115-P3
G9
WM-115 Vemurafenib PF562271 1.000 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 423.89 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 108.70 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 368.15
WM115-P3
G8