Phosphorylation state and protein levels measured by imaging in BRAF(V600E/D) melanoma cell lines following treatment with combinations of two compounds (immunofluorescence dataset 1 of 4) - Dataset (ID:20274)

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Cell Small Molecule 1 Small Molecule 2 Small Mol 1 Concentration Small Mol 2 Concentration Small Mol Conc Unit Time Point Time Point Unit Replicate Primary Antibody (488) Secondary Antibody (488) Protein Detected (488) Mean Fluorescence Intensity (488) Primary Antibody (568) Secondary Antibody (568) Protein Detected (568) Mean Fluorescence Intensity (568) Primary Antibody (647) Secondary Antibody (647) Protein Detected (647) Mean Fluorescence Intensity (647) Plate Name Plate Well
A-375 Vemurafenib Dasatinib 0.316 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 348.98 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 146.33 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 218.98
A375-P4
C10
WM-115 Vemurafenib JNK-IN-8 1.000 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 206.24 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 78.96 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 51.00
WM115-P10
D8
A-375 Vemurafenib Palbociclib 1.000 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 246.62 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 114.97 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 222.09
A375-P10
D10
A-375 Vemurafenib Saracatinib 0.000 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 2084.99 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 403.10 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 190.22
A375-P10
B2
A-375 Vemurafenib 0.316
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 684.49 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 172.91 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 357.40
A375-P4
C2
COLO 858 Vemurafenib Dasatinib 1.000 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 457.37 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 167.03 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 97.78
Colo858-P4
G10
A-375 Vemurafenib 0.316
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 688.95 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 166.69 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 363.53
A375-P4
C3
COLO 858 Vemurafenib Trametinib 1.000 0.200
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 488.28 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 120.56 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 377.84
Colo858-P4
G4
A-375 Vemurafenib Trametinib 0.316 0.200
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 232.02 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 123.94 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 304.95
A375-P4
C4
WM-115 Vemurafenib Pictilisib 0.000 3.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 535.58 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 149.41 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 138.23
WM115-P10
E5
COLO 858 Vemurafenib JNK-IN-8 1.000 3.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 314.27 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 161.24 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 74.70
Colo858-P10
G9
COLO 858 Vemurafenib Palbociclib 0.000 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 1123.26 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 197.24 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 99.59
Colo858-P10
E10
COLO 858 Vemurafenib Defactinib 0.316 3.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 324.24 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 134.44 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 221.29
Colo858-P4
F7
COLO 858 Vemurafenib AZD8055 0.316 0.200
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 90.47 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 111.48 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 341.30
Colo858-P10
C6
A-375 Vemurafenib JNK-IN-8 0.316 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 411.11 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 156.52 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 84.74
A375-P10
C9
COLO 858 Vemurafenib Palbociclib 1.000 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 -5.82 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 126.17 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 354.21
Colo858-P10
D11
COLO 858 Vemurafenib PF562271 0.316 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 475.47 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 151.80 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 312.91
Colo858-P4
F8
COLO 858 Vemurafenib Trametinib 1.000 0.600
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 402.07 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 121.59 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 381.76
Colo858-P4
G5
COLO 858 Vemurafenib Dasatinib 0.316 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 599.47 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 201.31 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 130.11
Colo858-P4
F10
COLO 858 Vemurafenib Palbociclib 0.316 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 -32.74 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 96.94 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 324.72
Colo858-P10
C10
COLO 858 Vemurafenib Dasatinib 0.316 3.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 703.20 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 198.29 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 91.17
Colo858-P4
F11
COLO 858 Vemurafenib Palbociclib 0.316 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 -64.90 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 106.44 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 296.56
Colo858-P10
C11
A-375 Vemurafenib JNK-IN-8 0.316 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 413.18 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 160.47 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 115.79
A375-P10
C8
COLO 858 Vemurafenib PF562271 0.000 3.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 1683.11 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 315.88 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 157.68
Colo858-P4
E9
COLO 858 Vemurafenib 0.316
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 987.46 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 196.33 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 354.14
Colo858-P4
F2