Phosphorylation state and protein levels measured by imaging in BRAF(V600E/D) melanoma cell lines following treatment with combinations of two compounds (immunofluorescence dataset 1 of 4) - Dataset (ID:20274)

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Cell Small Molecule 1 Small Molecule 2 Small Mol 1 Concentration Small Mol 2 Concentration Small Mol Conc Unit Time Point Time Point Unit Replicate Primary Antibody (488) Secondary Antibody (488) Protein Detected (488) Mean Fluorescence Intensity (488) Primary Antibody (568) Secondary Antibody (568) Protein Detected (568) Mean Fluorescence Intensity (568) Primary Antibody (647) Secondary Antibody (647) Protein Detected (647) Mean Fluorescence Intensity (647) Plate Name Plate Well
COLO 858 Vemurafenib Palbociclib 0.000 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 1145.34 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 202.17 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 96.67
Colo858-P10
B10
A-375 Vemurafenib Pictilisib 1.000 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 480.09 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 167.47 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 215.99
A375-P10
D5
COLO 858 Vemurafenib Saracatinib 0.000 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 2470.40 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 370.68 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 125.42
Colo858-P10
B3
COLO 858 Vemurafenib Defactinib 1.000 3.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 411.59 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 129.73 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 198.34
Colo858-P4
G7
COLO 858 Vemurafenib Defactinib 1.000 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 489.69 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 158.65 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 265.38
Colo858-P4
G6
COLO 858 Vemurafenib PF562271 1.000 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 372.17 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 119.07 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 261.05
Colo858-P4
G8
COLO 858 Vemurafenib Trametinib 1.000 0.200
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 488.28 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 120.56 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 377.84
Colo858-P4
G4
A-375 Vemurafenib Pictilisib 1.000 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 488.77 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 173.57 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 268.96
A375-P10
D4
COLO 858 Vemurafenib Trametinib 1.000 0.600
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 402.07 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 121.59 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 381.76
Colo858-P4
G5
COLO 858 Vemurafenib PF562271 1.000 3.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 301.00 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 131.34 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 257.41
Colo858-P4
G9
COLO 858 Vemurafenib Dasatinib 1.000 3.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 435.79 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 183.36 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 80.88
Colo858-P4
G11
COLO 858 Vemurafenib Dasatinib 1.000 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 457.37 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 167.03 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 97.78
Colo858-P4
G10
A-375 Vemurafenib Palbociclib 0.000 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 745.75 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 167.99 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 134.37
A375-P10
B10
A-375 Vemurafenib Trametinib 0.000 0.200
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 230.23 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 126.27 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 293.14
A375-P4
B4
COLO 858 Vemurafenib 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 694.26 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 360.82 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 347.86
Colo858-P4
G2
A-375 Vemurafenib Trametinib 0.000 0.600
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 260.65 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 134.67 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 350.66
A375-P4
B5
WM-115 Vemurafenib Palbociclib 0.000 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 109.09 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 69.20 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 60.22
WM115-P10
B11
A-375 Vemurafenib Defactinib 0.000 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 2059.99 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 371.47 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 198.06
A375-P4
B6
WM-115 Vemurafenib Saracatinib 0.000 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 588.33 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 160.22 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 96.17
WM115-P10
B3
COLO 858 Vemurafenib Pictilisib 0.000 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 1919.44 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 291.57 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 113.37
Colo858-P10
B4
A-375 Vemurafenib Palbociclib 0.000 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 573.25 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 137.42 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 113.14
A375-P10
B11
A-375 Vemurafenib PF562271 0.000 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 1984.98 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 378.90 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 182.56
A375-P4
B8
WM-115 Vemurafenib JNK-IN-8 0.000 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 866.90 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 214.45 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 72.76
WM115-P10
B8
A-375 Vemurafenib Saracatinib 1.000 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 485.65 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 175.90 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 226.89
A375-P10
D3
COLO 858 Vemurafenib PF562271 0.316 3.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 422.05 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 130.92 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 244.29
Colo858-P4
F9