Phosphorylation state and protein levels measured by imaging in BRAF(V600E/D) melanoma cell lines following treatment with combinations of two compounds (immunofluorescence dataset 1 of 4) - Dataset (ID:20274)

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Cell Small Molecule 1 Small Molecule 2 Small Mol 1 Concentration Small Mol 2 Concentration Small Mol Conc Unit Time Point Time Point Unit Replicate Primary Antibody (488) Secondary Antibody (488) Protein Detected (488) Mean Fluorescence Intensity (488) Primary Antibody (568) Secondary Antibody (568) Protein Detected (568) Mean Fluorescence Intensity (568) Primary Antibody (647) Secondary Antibody (647) Protein Detected (647) Mean Fluorescence Intensity (647) Plate Name Plate Well
WM-115 Vemurafenib 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 534.38 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 108.97 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 504.13
WM115-P3
D2
WM-115 Vemurafenib 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 474.36 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 105.22 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 493.87
WM115-P3
D3
WM-115 Vemurafenib Trametinib 1.000 0.600
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 310.66 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 114.38 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 485.74
WM115-P3
D5
WM-115 Vemurafenib Defactinib 1.000 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 228.98 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 92.30 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 518.23
WM115-P3
D6
A-375 Vemurafenib Saracatinib 0.000 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 2123.95 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 394.84 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 232.89
A375-P9
B2
A-375 Vemurafenib Pictilisib 0.000 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 1855.29 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 354.28 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 322.58
A375-P9
B4
A-375 Vemurafenib Palbociclib 1.000 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 183.33 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 90.28 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 232.77
A375-P9
D11
A-375 Vemurafenib JNK-IN-8 1.000 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 326.16 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 102.57 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 297.95
A375-P9
G8
A-375 Vemurafenib Palbociclib 0.000 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 580.03 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 130.73 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 143.65
A375-P9
B11
COLO 858 Vemurafenib 0.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 2417.04 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 305.53 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 131.05
Colo858-P3
B2
A-375 Vemurafenib Palbociclib 0.000 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 798.61 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 172.50 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 224.54
A375-P9
B10
COLO 858 Vemurafenib Trametinib 0.000 0.200
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 402.10 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 160.23 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 920.59
Colo858-P3
B4
A-375 Vemurafenib Saracatinib 0.000 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 2249.34 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 403.96 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 236.71
A375-P9
B3
COLO 858 Vemurafenib PF562271 0.000 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 2469.24 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 302.08 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 166.15
Colo858-P3
B8
COLO 858 Vemurafenib Dasatinib 0.316 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 641.27 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 182.89 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 187.13
Colo858-P3
C10
COLO 858 Vemurafenib Dasatinib 0.316 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 873.02 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 240.79 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 169.62
Colo858-P3
C11
COLO 858 Vemurafenib Trametinib 0.316 0.200
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 430.56 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 152.66 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 990.88
Colo858-P3
C4
COLO 858 Vemurafenib Trametinib 0.316 0.600
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 392.14 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 142.60 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 946.80
Colo858-P3
C5
COLO 858 Vemurafenib Defactinib 0.316 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 328.77 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 155.25 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 545.11
Colo858-P3
C7
COLO 858 Vemurafenib PF562271 0.316 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 385.79 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 155.04 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 655.95
Colo858-P3
C8
A-375 Vemurafenib AZD8055 0.000 0.600
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 1278.24 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 266.10 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 287.39
A375-P9
B7
A-375 Vemurafenib JNK-IN-8 0.000 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 1646.35 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 314.06 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 227.19
A375-P9
B9
COLO 858 Vemurafenib Trametinib 1.000 0.200
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 499.48 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 165.16 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 1013.30
Colo858-P3
D4
WM-115 Vemurafenib Trametinib 0.000 0.200
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 454.23 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 106.23 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 478.03
WM115-P3
E4
WM-115 Vemurafenib 0.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 1020.88 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 220.00 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 297.91
WM115-P3
E3