Phosphorylation state and protein levels measured by imaging in BRAF(V600E/D) melanoma cell lines following treatment with combinations of two compounds (immunofluorescence dataset 1 of 4) - Dataset (ID:20274)

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Cell Small Molecule 1 Small Molecule 2 Small Mol 1 Concentration Small Mol 2 Concentration Small Mol Conc Unit Time Point Time Point Unit Replicate Primary Antibody (488) Secondary Antibody (488) Protein Detected (488) Mean Fluorescence Intensity (488) Primary Antibody (568) Secondary Antibody (568) Protein Detected (568) Mean Fluorescence Intensity (568) Primary Antibody (647) Secondary Antibody (647) Protein Detected (647) Mean Fluorescence Intensity (647) Plate Name Plate Well
COLO 858 Vemurafenib Pictilisib 1.000 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 251.38 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 166.68 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 483.43
Colo858-P9
D5
COLO 858 Vemurafenib AZD8055 1.000 0.200
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 293.01 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 180.96 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 792.68
Colo858-P9
D6
COLO 858 Vemurafenib AZD8055 1.000 0.600
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 360.69 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 174.53 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 813.43
Colo858-P9
D7
COLO 858 Vemurafenib JNK-IN-8 1.000 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 247.27 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 169.05 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 359.05
Colo858-P9
D8
COLO 858 Vemurafenib JNK-IN-8 1.000 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 319.66 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 167.76 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 297.48
Colo858-P9
D9
COLO 858 Vemurafenib Palbociclib 0.000 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 1217.43 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 210.54 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 181.92
Colo858-P9
E10
COLO 858 Vemurafenib Palbociclib 0.000 3.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 785.10 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 180.24 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 189.75
Colo858-P9
E11
COLO 858 Vemurafenib Saracatinib 0.000 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 2484.31 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 351.08 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 109.58
Colo858-P9
E2
COLO 858 Vemurafenib Saracatinib 0.000 3.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 2762.32 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 383.98 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 125.22
Colo858-P9
E3
COLO 858 Vemurafenib Pictilisib 0.000 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 2097.61 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 302.09 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 153.69
Colo858-P9
E4
COLO 858 Vemurafenib Pictilisib 0.000 3.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 2047.57 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 286.72 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 162.29
Colo858-P9
E5
COLO 858 Vemurafenib AZD8055 0.000 0.200
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 1564.84 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 230.30 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 166.92
Colo858-P9
E6
COLO 858 Vemurafenib AZD8055 0.000 0.600
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 863.53 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 158.60 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 99.17
Colo858-P9
E7
COLO 858 Vemurafenib JNK-IN-8 0.000 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 2263.15 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 326.36 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 180.07
Colo858-P9
E8
COLO 858 Vemurafenib JNK-IN-8 0.000 3.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 2609.00 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 359.04 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 207.86
Colo858-P9
E9
COLO 858 Vemurafenib Palbociclib 0.316 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 75.79 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 117.87 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 622.25
Colo858-P9
F10
COLO 858 Vemurafenib Palbociclib 0.316 3.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 36.38 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 123.60 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 628.22
Colo858-P9
F11
COLO 858 Vemurafenib Saracatinib 0.316 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 664.10 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 220.19 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 549.42
Colo858-P9
F2
COLO 858 Vemurafenib Saracatinib 0.316 3.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 622.82 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 222.22 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 521.65
Colo858-P9
F3
COLO 858 Vemurafenib Pictilisib 0.316 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 587.51 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 222.89 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 543.83
Colo858-P9
F4
COLO 858 Vemurafenib Pictilisib 0.316 3.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 373.45 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 157.06 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 491.11
Colo858-P9
F5
COLO 858 Vemurafenib AZD8055 0.316 0.200
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 239.05 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 123.69 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 665.48
Colo858-P9
F6
COLO 858 Vemurafenib AZD8055 0.316 0.600
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 345.13 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 143.25 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 575.18
Colo858-P9
F7
COLO 858 Vemurafenib JNK-IN-8 0.316 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 532.79 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 177.02 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 248.12
Colo858-P9
F8
COLO 858 Vemurafenib JNK-IN-8 0.316 3.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 538.05 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 185.81 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 204.99
Colo858-P9
F9