Phosphorylation state and protein levels measured by imaging in BRAF(V600E/D) melanoma cell lines following treatment with combinations of two compounds (immunofluorescence dataset 1 of 4) - Dataset (ID:20274)

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Cell Small Molecule 1 Small Molecule 2 Small Mol 1 Concentration Small Mol 2 Concentration Small Mol Conc Unit Time Point Time Point Unit Replicate Primary Antibody (488) Secondary Antibody (488) Protein Detected (488) Mean Fluorescence Intensity (488) Primary Antibody (568) Secondary Antibody (568) Protein Detected (568) Mean Fluorescence Intensity (568) Primary Antibody (647) Secondary Antibody (647) Protein Detected (647) Mean Fluorescence Intensity (647) Plate Name Plate Well
WM-115 Vemurafenib Dasatinib 0.316 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 527.66 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 148.90 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 248.29
WM115-P3
C10
WM-115 Vemurafenib Dasatinib 0.316 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 399.49 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 123.94 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 257.51
WM115-P3
C11
WM-115 Vemurafenib 0.316
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 657.69 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 132.47 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 534.52
WM115-P3
C2
WM-115 Vemurafenib 0.316
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 529.42 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 135.37 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 552.89
WM115-P3
C3
WM-115 Vemurafenib Trametinib 0.316 0.200
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 437.02 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 140.81 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 491.42
WM115-P3
C4
WM-115 Vemurafenib Trametinib 0.316 0.600
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 399.88 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 131.14 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 491.50
WM115-P3
C5
WM-115 Vemurafenib Defactinib 0.316 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 387.18 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 137.65 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 523.09
WM115-P3
C6
WM-115 Vemurafenib Defactinib 0.316 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 402.24 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 136.00 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 494.72
WM115-P3
C7
WM-115 Vemurafenib PF562271 0.316 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 416.27 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 128.04 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 457.06
WM115-P3
C8
WM-115 Vemurafenib PF562271 0.316 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 248.74 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 107.79 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 179.07
WM115-P3
C9
WM-115 Vemurafenib Dasatinib 1.000 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 451.42 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 127.73 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 288.35
WM115-P3
D10
WM-115 Vemurafenib Dasatinib 1.000 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 455.70 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 126.94 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 285.61
WM115-P3
D11
WM-115 Vemurafenib 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 534.38 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 108.97 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 504.13
WM115-P3
D2
WM-115 Vemurafenib 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 474.36 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 105.22 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 493.87
WM115-P3
D3
WM-115 Vemurafenib Trametinib 1.000 0.200
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 376.53 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 100.56 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 459.53
WM115-P3
D4
WM-115 Vemurafenib Trametinib 1.000 0.600
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 310.66 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 114.38 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 485.74
WM115-P3
D5
WM-115 Vemurafenib Defactinib 1.000 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 228.98 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 92.30 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 518.23
WM115-P3
D6
WM-115 Vemurafenib Defactinib 1.000 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 237.71 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 91.67 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 450.80
WM115-P3
D7
WM-115 Vemurafenib PF562271 1.000 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 277.98 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 96.00 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 390.35
WM115-P3
D8
WM-115 Vemurafenib PF562271 1.000 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 258.39 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 93.53 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 177.11
WM115-P3
D9
WM-115 Vemurafenib Dasatinib 0.000 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 670.41 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 170.03 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 113.14
WM115-P3
E10
WM-115 Vemurafenib Dasatinib 0.000 3.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 639.56 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 178.56 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 176.26
WM115-P3
E11
WM-115 Vemurafenib 0.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 977.03 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 201.87 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 276.36
WM115-P3
E2
WM-115 Vemurafenib 0.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 1020.88 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 220.00 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 297.91
WM115-P3
E3
WM-115 Vemurafenib Trametinib 0.000 0.200
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 454.23 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 106.23 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 478.03
WM115-P3
E4