Phosphorylation state and protein levels measured by imaging in BRAF(V600E/D) melanoma cell lines following treatment with combinations of two compounds (immunofluorescence dataset 1 of 4) - Dataset (ID:20274)

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Cell Small Molecule 1 Small Molecule 2 Small Mol 1 Concentration Small Mol 2 Concentration Small Mol Conc Unit Time Point Time Point Unit Replicate Primary Antibody (488) Secondary Antibody (488) Protein Detected (488) Mean Fluorescence Intensity (488) Primary Antibody (568) Secondary Antibody (568) Protein Detected (568) Mean Fluorescence Intensity (568) Primary Antibody (647) Secondary Antibody (647) Protein Detected (647) Mean Fluorescence Intensity (647) Plate Name Plate Well
WM-115 Vemurafenib Dasatinib 1.000 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 476.39 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 124.76 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 447.86
WM115-P2
D10
WM-115 Vemurafenib Palbociclib 1.000 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 147.10 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 61.29 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 375.03
WM115-P9
D10
WM-115 Vemurafenib Palbociclib 1.000 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 186.36 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 74.65 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 642.16
WM115-P8
D10
WM-115 Vemurafenib Dasatinib 1.000 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 323.70 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 105.77 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 117.54
WM115-P4
D10
COLO 858 Vemurafenib Dasatinib 1.000 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 631.13 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 190.51 NGFR rabbit IgG(H+L) / donkey NGFR 45.27
Colo858-P1
D11
WM-115 Vemurafenib Dasatinib 1.000 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 455.70 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 126.94 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 285.61
WM115-P3
D11
A-375 Vemurafenib Palbociclib 1.000 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 183.33 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 90.28 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 232.77
A375-P9
D11
COLO 858 Vemurafenib Dasatinib 1.000 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 640.45 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 179.90 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 721.38
Colo858-P2
D11
COLO 858 Vemurafenib Dasatinib 1.000 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 442.01 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 173.57 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 141.54
Colo858-P3
D11
COLO 858 Vemurafenib Palbociclib 1.000 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 75.05 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 123.44 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 740.29
Colo858-P9
D11
A-375 Vemurafenib Dasatinib 1.000 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 428.90 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 155.70 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 377.11
A375-P3
D11
WM-115 Vemurafenib Dasatinib 1.000 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 466.73 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 136.01 NGFR rabbit IgG(H+L) / donkey NGFR 1258.98
WM115-P1
D11
A-375 Vemurafenib Palbociclib 1.000 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 303.56 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 110.58 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 1046.90
A375-P8
D11
COLO 858 Vemurafenib Dasatinib 1.000 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 487.10 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 166.79 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 79.46
Colo858-P4
D11
WM-115 Vemurafenib Dasatinib 1.000 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 439.93 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 133.97 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 473.71
WM115-P2
D11
A-375 Vemurafenib Palbociclib 1.000 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 150.26 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 90.18 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 169.63
A375-P10
D11
A-375 Vemurafenib Palbociclib 1.000 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 443.96 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 189.38 NGFR rabbit IgG(H+L) / donkey NGFR 431.55
A375-P7
D11
COLO 858 Vemurafenib Palbociclib 1.000 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 -5.82 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 126.17 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 354.21
Colo858-P10
D11
A-375 Vemurafenib Dasatinib 1.000 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 499.71 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 175.91 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 1034.98
A375-P2
D11
A-375 Vemurafenib Dasatinib 1.000 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 540.13 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 175.10 NGFR rabbit IgG(H+L) / donkey NGFR 180.58
A375-P1
D11
WM-115 Vemurafenib Palbociclib 1.000 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 73.48 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 51.63 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 142.88
WM115-P10
D11
COLO 858 Vemurafenib Palbociclib 1.000 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 142.44 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 108.82 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 233.16
Colo858-P8
D11
WM-115 Vemurafenib Palbociclib 1.000 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 159.56 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 67.99 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 645.54
WM115-P8
D11
WM-115 Vemurafenib Palbociclib 1.000 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 254.15 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 92.86 NGFR rabbit IgG(H+L) / donkey NGFR 2118.67
WM115-P7
D11
WM-115 Vemurafenib Dasatinib 1.000 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 292.02 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 108.65 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 117.09
WM115-P4
D11