Phosphorylation state and protein levels measured by imaging in BRAF(V600E/D) melanoma cell lines following treatment with combinations of two compounds (immunofluorescence dataset 1 of 4) - Dataset (ID:20274)

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Cell Small Molecule 1 Small Molecule 2 Small Mol 1 Concentration Small Mol 2 Concentration Small Mol Conc Unit Time Point Time Point Unit Replicate Primary Antibody (488) Secondary Antibody (488) Protein Detected (488) Mean Fluorescence Intensity (488) Primary Antibody (568) Secondary Antibody (568) Protein Detected (568) Mean Fluorescence Intensity (568) Primary Antibody (647) Secondary Antibody (647) Protein Detected (647) Mean Fluorescence Intensity (647) Plate Name Plate Well
WM-115 Vemurafenib JNK-IN-8 0.000 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 705.77 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 198.34 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 186.90
WM115-P9
B9
WM-115 Vemurafenib JNK-IN-8 0.000 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 654.58 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 191.24 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 55.00
WM115-P10
B9
A-375 Vemurafenib PF562271 0.000 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 1103.99 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 207.56 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 188.71
A375-P3
B9
COLO 858 Vemurafenib PF562271 0.000 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 2017.22 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 307.47 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 141.78
Colo858-P4
B9
COLO 858 Vemurafenib PF562271 0.000 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 2172.45 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 364.23 NGFR rabbit IgG(H+L) / donkey NGFR 74.98
Colo858-P1
B9
WM-115 Vemurafenib PF562271 0.000 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 400.82 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 163.70 NGFR rabbit IgG(H+L) / donkey NGFR 1013.88
WM115-P1
B9
COLO 858 Vemurafenib PF562271 0.000 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 2296.04 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 351.54 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 298.57
Colo858-P3
B9
COLO 858 Vemurafenib PF562271 0.000 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 1929.29 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 342.40 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 5195.37
Colo858-P2
B9
A-375 Vemurafenib JNK-IN-8 0.000 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 1789.77 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 386.63 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 6862.16
A375-P8
B9
A-375 Vemurafenib JNK-IN-8 0.000 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 1646.35 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 314.06 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 227.19
A375-P9
B9
COLO 858 Vemurafenib JNK-IN-8 0.000 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 2571.01 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 408.55 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 5297.17
Colo858-P8
B9
A-375 Vemurafenib JNK-IN-8 0.000 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 1645.39 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 328.55 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 123.98
A375-P10
B9
A-375 Vemurafenib PF562271 0.000 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 1339.47 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 253.18 NGFR rabbit IgG(H+L) / donkey NGFR 154.21
A375-P1
B9
COLO 858 Vemurafenib JNK-IN-8 0.000 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 2776.56 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 389.01 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 288.12
Colo858-P9
B9
A-375 Vemurafenib PF562271 0.000 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 1538.44 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 307.16 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 7914.56
A375-P2
B9
WM-115 Vemurafenib Palbociclib 0.316 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 274.29 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 100.46 NGFR rabbit IgG(H+L) / donkey NGFR 1930.11
WM115-P7
C10
WM-115 Vemurafenib Dasatinib 0.316 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 371.56 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 124.27 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 117.18
WM115-P4
C10
A-375 Vemurafenib Dasatinib 0.316 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 348.98 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 146.33 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 218.98
A375-P4
C10
COLO 858 Vemurafenib Palbociclib 0.316 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 838.35 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 139.35 NGFR rabbit IgG(H+L) / donkey NGFR 802.84
Colo858-P7
C10
COLO 858 Vemurafenib Palbociclib 0.316 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 -32.74 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 96.94 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 324.72
Colo858-P10
C10
WM-115 Vemurafenib Dasatinib 0.316 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 479.77 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 132.80 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 562.92
WM115-P2
C10
WM-115 Vemurafenib Dasatinib 0.316 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 527.66 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 148.90 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 248.29
WM115-P3
C10
A-375 Vemurafenib Palbociclib 0.316 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 472.08 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 159.91 NGFR rabbit IgG(H+L) / donkey NGFR 749.94
A375-P7
C10
A-375 Vemurafenib Dasatinib 0.316 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 538.29 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 166.43 NGFR rabbit IgG(H+L) / donkey NGFR 167.43
A375-P1
C10
A-375 Vemurafenib Palbociclib 0.316 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 368.02 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 126.53 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 2094.50
A375-P8
C10