Phosphorylation state and protein levels measured by imaging in BRAF(V600E/D) melanoma cell lines following treatment with combinations of two compounds (immunofluorescence dataset 1 of 4) - Dataset (ID:20274)

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Cell Small Molecule 1 Small Molecule 2 Small Mol 1 Concentration Small Mol 2 Concentration Small Mol Conc Unit Time Point Time Point Unit Replicate Primary Antibody (488) Secondary Antibody (488) Protein Detected (488) Mean Fluorescence Intensity (488) Primary Antibody (568) Secondary Antibody (568) Protein Detected (568) Mean Fluorescence Intensity (568) Primary Antibody (647) Secondary Antibody (647) Protein Detected (647) Mean Fluorescence Intensity (647) Plate Name Plate Well
COLO 858 Vemurafenib Trametinib 0.000 0.200
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 487.69 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 174.14 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 246.52
Colo858-P2
B4
A-375 Vemurafenib Saracatinib 0.316 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 465.64 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 166.19 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 247.07
A375-P10
C3
A-375 Vemurafenib JNK-IN-8 0.316 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 357.45 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 128.98 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 247.37
A375-P9
C9
COLO 858 Vemurafenib JNK-IN-8 0.316 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 532.79 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 177.02 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 248.12
Colo858-P9
F8
WM-115 Vemurafenib Dasatinib 0.316 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 527.66 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 148.90 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 248.29
WM115-P3
C10
COLO 858 Vemurafenib Defactinib 0.316 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 543.72 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 167.84 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 248.68
Colo858-P4
C6
A-375 Vemurafenib Defactinib 0.316 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 476.63 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 143.31 NGFR rabbit IgG(H+L) / donkey NGFR 248.80
A375-P1
C7
WM-115 Vemurafenib 0.316
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 453.28 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 118.64 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 249.06
WM115-P4
C3
COLO 858 Vemurafenib Defactinib 0.316 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 422.62 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 168.94 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 249.19
Colo858-P4
F6
COLO 858 Vemurafenib Saracatinib 1.000 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 499.63 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 170.39 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 249.37
Colo858-P8
G2
COLO 858 Vemurafenib Saracatinib 1.000 3.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 536.35 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 165.83 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 249.75
Colo858-P10
G3
COLO 858 Vemurafenib PF562271 0.316 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 326.90 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 155.90 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 250.06
Colo858-P4
C9
WM-115 Vemurafenib Defactinib 0.000 3.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 475.64 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 155.71 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 250.67
WM115-P3
E7
A-375 Vemurafenib JNK-IN-8 0.000 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 2058.90 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 364.89 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 252.32
A375-P9
E8
A-375 Vemurafenib PF562271 0.000 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 2421.26 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 433.32 NGFR rabbit IgG(H+L) / donkey NGFR 252.56
A375-P1
E8
COLO 858 Vemurafenib Trametinib 1.000 0.200
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 432.54 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 151.03 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 253.26
Colo858-P2
D4
A-375 Vemurafenib 0.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 2409.02 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 391.32 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 254.09
A375-P4
B3
COLO 858 Vemurafenib JNK-IN-8 1.000 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 1067.04 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 182.92 NGFR rabbit IgG(H+L) / donkey NGFR 254.47
Colo858-P7
D9
WM-115 Vemurafenib Defactinib 0.000 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 625.27 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 177.31 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 254.54
WM115-P3
B7
WM-115 Vemurafenib PF562271 0.000 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 644.33 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 162.03 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 254.66
WM115-P3
E8
A-375 Vemurafenib PF562271 0.000 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 2137.32 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 362.84 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 255.30
A375-P3
E8
A-375 Vemurafenib JNK-IN-8 1.000 3.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 379.81 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 117.13 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 256.19
A375-P9
G9
COLO 858 Vemurafenib Palbociclib 1.000 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 146.77 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 106.96 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 257.34
Colo858-P8
G10
A-375 Vemurafenib PF562271 0.000 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 2497.37 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 448.69 NGFR rabbit IgG(H+L) / donkey NGFR 257.39
A375-P1
B8
COLO 858 Vemurafenib PF562271 1.000 3.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 301.00 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 131.34 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 257.41
Colo858-P4
G9