Phosphorylation state and protein levels measured by imaging in BRAF(V600E/D) melanoma cell lines following treatment with combinations of two compounds (immunofluorescence dataset 1 of 4) - Dataset (ID:20274)

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Cell Small Molecule 1 Small Molecule 2 Small Mol 1 Concentration Small Mol 2 Concentration Small Mol Conc Unit Time Point Time Point Unit Replicate Primary Antibody (488) Secondary Antibody (488) Protein Detected (488) Mean Fluorescence Intensity (488) Primary Antibody (568) Secondary Antibody (568) Protein Detected (568) Mean Fluorescence Intensity (568) Primary Antibody (647) Secondary Antibody (647) Protein Detected (647) Mean Fluorescence Intensity (647) Plate Name Plate Well
COLO 858 Vemurafenib AZD8055 0.000 0.200
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 1310.64 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 188.82 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 132.94
Colo858-P8
E6
COLO 858 Vemurafenib Defactinib 1.000 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 303.50 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 112.14 NGFR rabbit IgG(H+L) / donkey NGFR 133.45
Colo858-P1
D7
COLO 858 Vemurafenib JNK-IN-8 0.000 3.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 3190.16 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 401.49 NGFR rabbit IgG(H+L) / donkey NGFR 133.47
Colo858-P7
E9
COLO 858 Vemurafenib Saracatinib 0.000 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 3359.90 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 399.82 NGFR rabbit IgG(H+L) / donkey NGFR 133.84
Colo858-P7
B3
A-375 Vemurafenib PF562271 0.000 3.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 823.97 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 170.97 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 134.09
A375-P4
E9
A-375 Vemurafenib Palbociclib 0.000 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 745.75 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 167.99 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 134.37
A375-P10
B10
COLO 858 Vemurafenib Defactinib 0.316 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 637.08 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 199.79 NGFR rabbit IgG(H+L) / donkey NGFR 134.41
Colo858-P1
C6
WM-115 Vemurafenib Palbociclib 1.000 3.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 67.64 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 50.32 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 134.52
WM115-P10
G11
WM-115 Vemurafenib Trametinib 0.316 0.600
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 201.43 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 79.20 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 134.69
WM115-P4
C5
A-375 Vemurafenib PF562271 0.000 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 787.92 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 188.07 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 135.23
A375-P4
B9
COLO 858 Vemurafenib 0.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 2508.82 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 298.52 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 135.52
Colo858-P3
E2
A-375 Vemurafenib PF562271 0.000 3.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 1095.66 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 214.29 NGFR rabbit IgG(H+L) / donkey NGFR 135.55
A375-P1
E9
COLO 858 Vemurafenib Dasatinib 0.000 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 3971.65 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 473.49 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 136.32
Colo858-P3
E10
WM-115 Vemurafenib 0.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 832.56 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 183.70 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 136.68
WM115-P4
B2
WM-115 Vemurafenib Saracatinib 0.000 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 712.23 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 152.78 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 136.83
WM115-P9
E2
COLO 858 Vemurafenib JNK-IN-8 0.000 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 3266.62 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 411.78 NGFR rabbit IgG(H+L) / donkey NGFR 137.05
Colo858-P7
B9
WM-115 Vemurafenib PF562271 0.000 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 393.81 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 144.62 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 137.51
WM115-P3
B9
WM-115 Vemurafenib Palbociclib 1.000 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 100.60 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 52.74 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 137.72
WM115-P10
G10
COLO 858 Vemurafenib Dasatinib 0.316 3.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 733.06 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 204.44 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 138.01
Colo858-P3
F11
COLO 858 Vemurafenib Saracatinib 0.000 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 3102.17 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 379.15 NGFR rabbit IgG(H+L) / donkey NGFR 138.08
Colo858-P7
E2
A-375 Vemurafenib Pictilisib 1.000 3.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 566.92 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 161.07 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 138.17
A375-P8
G5
A-375 Vemurafenib Dasatinib 0.316 3.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 523.10 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 171.20 NGFR rabbit IgG(H+L) / donkey NGFR 138.23
A375-P1
F11
WM-115 Vemurafenib Pictilisib 0.000 3.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 535.58 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 149.41 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 138.23
WM115-P10
E5
WM-115 Vemurafenib Saracatinib 0.000 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 647.13 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 167.16 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 140.20
WM115-P9
B3
A-375 Vemurafenib JNK-IN-8 0.316 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 477.45 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 147.57 NGFR rabbit IgG(H+L) / donkey NGFR 140.32
A375-P7
C9