Phosphorylation state and protein levels measured by imaging in BRAF(V600E/D) melanoma cell lines following treatment with combinations of two compounds (immunofluorescence dataset 1 of 4) - Dataset (ID:20274)

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Cell Small Molecule 1 Small Molecule 2 Small Mol 1 Concentration Small Mol 2 Concentration Small Mol Conc Unit Time Point Time Point Unit Replicate Primary Antibody (488) Secondary Antibody (488) Protein Detected (488) Mean Fluorescence Intensity (488) Primary Antibody (568) Secondary Antibody (568) Protein Detected (568) Mean Fluorescence Intensity (568) Primary Antibody (647) Secondary Antibody (647) Protein Detected (647) Mean Fluorescence Intensity (647) Plate Name Plate Well
WM-115 Vemurafenib Pictilisib 1.000 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 425.95 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 85.95 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 161.81
WM115-P10
G4
WM-115 Vemurafenib Pictilisib 1.000 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 385.98 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 86.02 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 343.20
WM115-P9
G4
WM-115 Vemurafenib JNK-IN-8 0.316 3.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 345.84 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 86.32 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 42.90
WM115-P10
F9
A-375 Vemurafenib Palbociclib 0.316 3.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 182.69 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 86.52 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 266.67
A375-P9
F11
WM-115 Vemurafenib AZD8055 0.316 0.600
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 755.26 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 86.53 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 468.23
WM115-P9
F7
WM-115 Vemurafenib AZD8055 1.000 0.200
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 573.82 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 86.77 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 490.85
WM115-P9
G6
WM-115 Vemurafenib AZD8055 1.000 0.200
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 617.87 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 86.86 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 28.01
WM115-P8
G6
WM-115 Vemurafenib PF562271 1.000 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 235.11 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 87.45 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 154.60
WM115-P4
G8
WM-115 Vemurafenib 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 399.46 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 87.77 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 201.01
WM115-P4
G3
WM-115 Vemurafenib Defactinib 1.000 3.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 249.46 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 88.02 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 160.63
WM115-P4
G7
WM-115 Vemurafenib PF562271 1.000 3.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 160.70 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 88.10 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 97.08
WM115-P4
G9
WM-115 Vemurafenib Defactinib 1.000 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 134.07 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 88.13 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 186.13
WM115-P4
D7
WM-115 Vemurafenib AZD8055 0.316 0.200
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 610.19 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 88.70 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 471.70
WM115-P9
C6
WM-115 Vemurafenib Pictilisib 1.000 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 379.44 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 88.99 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 354.48
WM115-P9
D4
WM-115 Vemurafenib JNK-IN-8 1.000 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 300.62 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 89.31 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 40.05
WM115-P10
D9
WM-115 Vemurafenib Palbociclib 0.000 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 157.69 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 89.34 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 67.20
WM115-P10
B10
WM-115 Vemurafenib Pictilisib 1.000 3.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 513.38 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 89.69 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 31.49
WM115-P8
G5
WM-115 Vemurafenib AZD8055 1.000 0.600
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 593.79 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 89.71 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 528.75
WM115-P9
D7
WM-115 Vemurafenib Saracatinib 0.316 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 408.29 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 89.79 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 390.46
WM115-P9
F2
WM-115 Vemurafenib AZD8055 1.000 0.200
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 514.99 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 89.87 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 30.79
WM115-P8
D6
A-375 Vemurafenib Palbociclib 1.000 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 150.26 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 90.18 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 169.63
A375-P10
D11
WM-115 Vemurafenib Defactinib 1.000 3.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 358.03 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 90.20 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 412.30
WM115-P3
G7
A-375 Vemurafenib Palbociclib 1.000 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 183.33 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 90.28 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 232.77
A375-P9
D11
WM-115 Vemurafenib Pictilisib 1.000 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 287.69 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 90.48 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 273.12
WM115-P9
D5
A-375 Vemurafenib Palbociclib 0.316 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 178.64 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 90.48 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 285.72
A375-P9
C11