Phosphorylation state and protein levels measured by imaging in BRAF(V600E/D) melanoma cell lines following treatment with combinations of two compounds (immunofluorescence dataset 1 of 4) - Dataset (ID:20274)

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Cell Small Molecule 1 Small Molecule 2 Small Mol 1 Concentration Small Mol 2 Concentration Small Mol Conc Unit Time Point Time Point Unit Replicate Primary Antibody (488) Secondary Antibody (488) Protein Detected (488) Mean Fluorescence Intensity (488) Primary Antibody (568) Secondary Antibody (568) Protein Detected (568) Mean Fluorescence Intensity (568) Primary Antibody (647) Secondary Antibody (647) Protein Detected (647) Mean Fluorescence Intensity (647) Plate Name Plate Well
WM-115 Vemurafenib PF562271 0.000 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 458.53 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 139.65 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 111.65
WM115-P4
E8
WM-115 Vemurafenib 0.316
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 629.57 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 139.69 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 1697.79
WM115-P2
C3
COLO 858 Vemurafenib Palbociclib 0.316 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 167.48 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 139.73 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 657.31
Colo858-P9
C10
A-375 Vemurafenib JNK-IN-8 1.000 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 441.74 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 139.78 NGFR rabbit IgG(H+L) / donkey NGFR 262.27
A375-P7
D9
COLO 858 Vemurafenib AZD8055 0.316 0.600
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 419.76 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 139.79 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 59.36
Colo858-P8
C7
A-375 Vemurafenib PF562271 0.316 3.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 321.66 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 139.80 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 224.82
A375-P4
F9
WM-115 Vemurafenib Dasatinib 0.316 3.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 480.31 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 139.83 NGFR rabbit IgG(H+L) / donkey NGFR 889.64
WM115-P1
F11
A-375 Vemurafenib Defactinib 0.316 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 364.31 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 139.86 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 286.08
A375-P4
F6
WM-115 Vemurafenib Saracatinib 1.000 3.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 608.80 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 140.11 NGFR rabbit IgG(H+L) / donkey NGFR 1797.50
WM115-P7
G3
A-375 Vemurafenib Dasatinib 0.316 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 352.46 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 140.18 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 207.24
A375-P4
F10
A-375 Vemurafenib Palbociclib 0.000 3.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 560.61 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 140.38 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 108.53
A375-P10
E11
A-375 Vemurafenib Saracatinib 1.000 3.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 538.87 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 140.47 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 538.00
A375-P8
G3
A-375 Vemurafenib Trametinib 0.316 0.200
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 302.60 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 140.61 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 828.66
A375-P3
C4
A-375 Vemurafenib Defactinib 1.000 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 308.19 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 140.67 NGFR rabbit IgG(H+L) / donkey NGFR 468.47
A375-P1
D6
WM-115 Vemurafenib 0.316
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 657.31 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 140.75 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 1503.46
WM115-P2
F3
WM-115 Vemurafenib Trametinib 0.316 0.200
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 437.02 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 140.81 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 491.42
WM115-P3
C4
WM-115 Vemurafenib Defactinib 1.000 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 316.47 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 140.81 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 992.77
WM115-P2
D6
WM-115 Vemurafenib Saracatinib 1.000 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 536.82 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 140.82 NGFR rabbit IgG(H+L) / donkey NGFR 2334.75
WM115-P7
G2
A-375 Vemurafenib Saracatinib 1.000 3.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 571.55 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 140.90 NGFR rabbit IgG(H+L) / donkey NGFR 209.37
A375-P7
G3
WM-115 Vemurafenib AZD8055 0.000 0.600
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 882.34 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 141.39 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 210.67
WM115-P9
E7
A-375 Vemurafenib Dasatinib 1.000 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 337.51 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 141.47 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 415.59
A375-P3
D10
WM-115 Vemurafenib Saracatinib 0.316 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 651.38 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 141.57 NGFR rabbit IgG(H+L) / donkey NGFR 1715.91
WM115-P7
C2
COLO 858 Vemurafenib Trametinib 0.316 0.600
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 389.65 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 141.61 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 275.98
Colo858-P2
C5
A-375 Vemurafenib Trametinib 0.000 0.200
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 316.96 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 141.63 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 839.25
A375-P3
B4
COLO 858 Vemurafenib JNK-IN-8 1.000 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 150.00 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 141.64 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 45.05
Colo858-P10
D9