Phosphorylation state and protein levels measured by imaging in BRAF(V600E/D) melanoma cell lines following treatment with combinations of two compounds (immunofluorescence dataset 1 of 4) - Dataset (ID:20274)

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Cell Small Molecule 1 Small Molecule 2 Small Mol 1 Concentration Small Mol 2 Concentration Small Mol Conc Unit Time Point Time Point Unit Replicate Primary Antibody (488) Secondary Antibody (488) Protein Detected (488) Mean Fluorescence Intensity (488) Primary Antibody (568) Secondary Antibody (568) Protein Detected (568) Mean Fluorescence Intensity (568) Primary Antibody (647) Secondary Antibody (647) Protein Detected (647) Mean Fluorescence Intensity (647) Plate Name Plate Well
A-375 Vemurafenib Palbociclib 1.000 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 246.62 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 114.97 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 222.09
A375-P10
D10
WM-115 Vemurafenib Trametinib 1.000 0.600
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 338.96 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 115.06 NGFR rabbit IgG(H+L) / donkey NGFR 3192.24
WM115-P1
D5
WM-115 Vemurafenib AZD8055 1.000 0.200
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 534.00 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 115.14 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 266.81
WM115-P10
D6
WM-115 Vemurafenib Palbociclib 0.316 3.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 312.05 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 115.19 NGFR rabbit IgG(H+L) / donkey NGFR 1560.44
WM115-P7
F11
WM-115 Vemurafenib JNK-IN-8 0.316 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 456.62 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 115.25 NGFR rabbit IgG(H+L) / donkey NGFR 1262.01
WM115-P7
F8
A-375 Vemurafenib Defactinib 0.316 3.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 296.09 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 115.34 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 240.34
A375-P4
F7
A-375 Vemurafenib Defactinib 1.000 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 303.07 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 115.50 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 227.55
A375-P4
G6
WM-115 Vemurafenib PF562271 0.000 3.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 313.91 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 115.64 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 120.95
WM115-P3
E9
WM-115 Vemurafenib Defactinib 1.000 3.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 473.91 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 115.67 NGFR rabbit IgG(H+L) / donkey NGFR 1519.03
WM115-P1
G7
WM-115 Vemurafenib Pictilisib 0.316 3.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 606.66 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 115.68 NGFR rabbit IgG(H+L) / donkey NGFR 959.04
WM115-P7
F5
WM-115 Vemurafenib Defactinib 0.316 3.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 488.37 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 115.79 NGFR rabbit IgG(H+L) / donkey NGFR 1294.99
WM115-P1
F7
WM-115 Vemurafenib Pictilisib 0.000 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 499.81 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 115.87 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 146.91
WM115-P9
B5
WM-115 Vemurafenib Palbociclib 0.000 3.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 305.69 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 116.06 NGFR rabbit IgG(H+L) / donkey NGFR 1300.91
WM115-P7
E11
A-375 Vemurafenib Saracatinib 1.000 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 383.99 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 116.42 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 206.01
A375-P10
G2
WM-115 Vemurafenib Defactinib 1.000 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 495.77 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 116.67 NGFR rabbit IgG(H+L) / donkey NGFR 2045.07
WM115-P1
G6
WM-115 Vemurafenib Dasatinib 0.316 3.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 364.00 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 116.70 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 634.04
WM115-P2
F11
COLO 858 Vemurafenib AZD8055 1.000 0.200
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 238.85 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 117.12 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 363.67
Colo858-P10
G6
A-375 Vemurafenib JNK-IN-8 1.000 3.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 379.81 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 117.13 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 256.19
A375-P9
G9
A-375 Vemurafenib Dasatinib 1.000 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 212.10 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 117.17 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 217.37
A375-P4
G10
COLO 858 Vemurafenib AZD8055 0.316 0.600
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 163.55 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 117.33 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 270.97
Colo858-P10
C7
A-375 Vemurafenib JNK-IN-8 1.000 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 348.73 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 117.36 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 318.34
A375-P9
D9
A-375 Vemurafenib Trametinib 0.000 0.600
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 171.18 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 117.55 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 365.44
A375-P4
E5
WM-115 Vemurafenib Pictilisib 0.316 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 491.21 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 117.62 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 47.00
WM115-P8
C5
A-375 Vemurafenib PF562271 1.000 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 212.72 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 117.70 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 207.21
A375-P4
D9
WM-115 Vemurafenib Saracatinib 0.316 3.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 479.32 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 117.79 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 317.57
WM115-P9
F3