Phosphorylation state and protein levels measured by imaging in BRAF(V600E/D) melanoma cell lines following treatment with combinations of two compounds (immunofluorescence dataset 1 of 4) - Dataset (ID:20274)

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Cell Small Molecule 1 Small Molecule 2 Small Mol 1 Concentration Small Mol 2 Concentration Small Mol Conc Unit Time Point Time Point Unit Replicate Primary Antibody (488) Secondary Antibody (488) Protein Detected (488) Mean Fluorescence Intensity (488) Primary Antibody (568) Secondary Antibody (568) Protein Detected (568) Mean Fluorescence Intensity (568) Primary Antibody (647) Secondary Antibody (647) Protein Detected (647) Mean Fluorescence Intensity (647) Plate Name Plate Well
COLO 858 Vemurafenib 0.316
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 1188.52 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 232.45 NGFR rabbit IgG(H+L) / donkey NGFR 555.83
Colo858-P1
F2
COLO 858 Vemurafenib 0.316
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 1193.30 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 206.40 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 350.18
Colo858-P4
C2
A-375 Vemurafenib PF562271 0.000 3.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 1198.87 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 189.88 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 186.48
A375-P3
E9
A-375 Vemurafenib AZD8055 0.000 0.600
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 1214.84 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 269.03 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 176.94
A375-P10
B7
COLO 858 Vemurafenib Pictilisib 0.316 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 1215.24 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 189.44 NGFR rabbit IgG(H+L) / donkey NGFR 547.76
Colo858-P7
C5
COLO 858 Vemurafenib Palbociclib 0.000 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 1217.43 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 210.54 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 181.92
Colo858-P9
E10
COLO 858 Vemurafenib 0.316
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 1228.10 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 356.29 NGFR rabbit IgG(H+L) / donkey NGFR 611.83
Colo858-P1
F3
WM-115 Vemurafenib Saracatinib 0.000 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 1228.57 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 363.06 NGFR rabbit IgG(H+L) / donkey NGFR 1118.25
WM115-P7
B2
A-375 Vemurafenib AZD8055 0.000 0.600
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 1255.45 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 286.02 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 186.22
A375-P10
E7
A-375 Vemurafenib Pictilisib 0.000 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 1276.95 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 295.45 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 259.89
A375-P9
B5
A-375 Vemurafenib AZD8055 0.000 0.600
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 1278.24 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 266.10 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 287.39
A375-P9
B7
A-375 Vemurafenib AZD8055 0.000 0.200
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 1296.78 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 279.58 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 298.21
A375-P9
E6
A-375 Vemurafenib AZD8055 0.000 0.200
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 1301.07 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 298.21 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 167.81
A375-P10
E6
COLO 858 Vemurafenib 0.316
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 1303.68 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 244.16 NGFR rabbit IgG(H+L) / donkey NGFR 525.63
Colo858-P1
C2
A-375 Vemurafenib AZD8055 0.000 0.600
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 1304.14 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 256.23 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 279.80
A375-P9
E7
COLO 858 Vemurafenib AZD8055 0.000 0.200
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 1310.64 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 188.82 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 132.94
Colo858-P8
E6
COLO 858 Vemurafenib Pictilisib 0.316 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 1311.46 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 207.38 NGFR rabbit IgG(H+L) / donkey NGFR 642.66
Colo858-P7
F4
COLO 858 Vemurafenib Saracatinib 1.000 3.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 1312.96 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 200.04 NGFR rabbit IgG(H+L) / donkey NGFR 329.77
Colo858-P7
G3
A-375 Vemurafenib Defactinib 0.000 3.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 1313.01 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 271.50 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 180.78
A375-P4
E7
A-375 Vemurafenib Pictilisib 0.000 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 1326.42 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 315.74 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 174.73
A375-P10
B5
A-375 Vemurafenib Defactinib 0.000 3.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 1330.25 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 270.77 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 275.47
A375-P3
E7
WM-115 Vemurafenib 0.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 1336.19 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 271.63 NGFR rabbit IgG(H+L) / donkey NGFR 1363.41
WM115-P1
B2
COLO 858 Vemurafenib Palbociclib 0.000 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 1339.44 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 236.98 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 267.54
Colo858-P9
B10
A-375 Vemurafenib PF562271 0.000 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 1339.47 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 253.18 NGFR rabbit IgG(H+L) / donkey NGFR 154.21
A375-P1
B9
COLO 858 Vemurafenib AZD8055 0.000 0.200
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 1343.04 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 187.72 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 68.93
Colo858-P10
B6