Phosphorylation state and protein levels measured by imaging in BRAF(V600E/D) melanoma cell lines following treatment with combinations of two compounds (immunofluorescence dataset 1 of 4) - Dataset (ID:20274)

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Cell Small Molecule 1 Small Molecule 2 Small Mol 1 Concentration Small Mol 2 Concentration Small Mol Conc Unit Time Point Time Point Unit Replicate Primary Antibody (488) Secondary Antibody (488) Protein Detected (488) Mean Fluorescence Intensity (488) Primary Antibody (568) Secondary Antibody (568) Protein Detected (568) Mean Fluorescence Intensity (568) Primary Antibody (647) Secondary Antibody (647) Protein Detected (647) Mean Fluorescence Intensity (647) Plate Name Plate Well
WM-115 Vemurafenib Palbociclib 1.000 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 147.10 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 61.29 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 375.03
WM115-P9
D10
WM-115 Vemurafenib Palbociclib 1.000 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 147.16 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 61.76 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 159.57
WM115-P10
D10
COLO 858 Vemurafenib JNK-IN-8 1.000 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 150.00 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 141.64 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 45.05
Colo858-P10
D9
WM-115 Vemurafenib Palbociclib 0.316 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 150.21 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 69.37 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 398.64
WM115-P9
C10
A-375 Vemurafenib Palbociclib 1.000 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 150.26 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 90.18 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 169.63
A375-P10
D11
WM-115 Vemurafenib Palbociclib 0.000 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 151.38 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 99.35 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 2839.07
WM115-P8
B11
WM-115 Vemurafenib Palbociclib 0.316 3.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 151.53 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 59.55 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 1027.76
WM115-P8
F11
WM-115 Vemurafenib Palbociclib 1.000 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 155.14 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 73.93 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 692.64
WM115-P8
G10
WM-115 Vemurafenib PF562271 0.316 3.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 156.06 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 80.75 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 83.91
WM115-P4
F9
WM-115 Vemurafenib Palbociclib 0.316 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 156.27 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 70.38 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 196.20
WM115-P10
C10
WM-115 Vemurafenib Palbociclib 0.000 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 157.69 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 89.34 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 67.20
WM115-P10
B10
WM-115 Vemurafenib Palbociclib 1.000 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 159.56 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 67.99 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 645.54
WM115-P8
D11
WM-115 Vemurafenib PF562271 1.000 3.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 160.70 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 88.10 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 97.08
WM115-P4
G9
COLO 858 Vemurafenib AZD8055 0.316 0.600
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 163.55 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 117.33 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 270.97
Colo858-P10
C7
WM-115 Vemurafenib Palbociclib 0.000 3.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 164.67 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 73.89 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 62.75
WM115-P10
E11
A-375 Vemurafenib Defactinib 1.000 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 165.74 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 130.82 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 499.78
A375-P3
D6
COLO 858 Vemurafenib Palbociclib 0.316 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 167.48 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 139.73 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 657.31
Colo858-P9
C10
WM-115 Vemurafenib PF562271 0.316 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 168.87 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 94.15 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 91.48
WM115-P4
C9
WM-115 Vemurafenib Palbociclib 0.000 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 170.14 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 76.83 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 109.85
WM115-P9
E10
A-375 Vemurafenib Trametinib 0.000 0.600
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 171.18 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 117.55 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 365.44
A375-P4
E5
A-375 Vemurafenib Palbociclib 1.000 3.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 172.27 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 95.77 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 180.14
A375-P10
G11
A-375 Vemurafenib Palbociclib 1.000 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 172.81 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 93.73 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 207.21
A375-P10
G10
WM-115 Vemurafenib Palbociclib 0.316 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 173.45 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 75.38 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 1286.81
WM115-P8
C10
WM-115 Vemurafenib Palbociclib 0.316 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 174.07 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 61.66 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 187.84
WM115-P10
F10
WM-115 Vemurafenib Palbociclib 0.000 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 177.77 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 107.45 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 2909.88
WM115-P8
B10