Phosphorylation state and protein levels measured by imaging in BRAF(V600E/D) melanoma cell lines following treatment with combinations of two compounds (immunofluorescence dataset 1 of 4) - Dataset (ID:20274)

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Cell Small Molecule 1 Small Molecule 2 Small Mol 1 Concentration Small Mol 2 Concentration Small Mol Conc Unit Time Point Time Point Unit Replicate Primary Antibody (488) Secondary Antibody (488) Protein Detected (488) Mean Fluorescence Intensity (488) Primary Antibody (568) Secondary Antibody (568) Protein Detected (568) Mean Fluorescence Intensity (568) Primary Antibody (647) Secondary Antibody (647) Protein Detected (647) Mean Fluorescence Intensity (647) Plate Name Plate Well
WM-115 Vemurafenib Trametinib 0.316 0.200
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 444.74 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 106.86 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 693.45
WM115-P2
F4
A-375 Vemurafenib Trametinib 0.000 0.600
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 445.42 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 156.24 NGFR rabbit IgG(H+L) / donkey NGFR 1195.67
A375-P1
E5
COLO 858 Vemurafenib Trametinib 0.316 0.200
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 445.89 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 133.24 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 403.95
Colo858-P4
C4
WM-115 Vemurafenib Trametinib 0.316 0.600
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 446.09 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 119.13 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 484.62
WM115-P3
F5
WM-115 Vemurafenib Trametinib 0.316 0.200
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 446.29 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 110.40 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 454.27
WM115-P3
F4
WM-115 Vemurafenib Defactinib 0.316 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 447.13 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 106.30 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 516.28
WM115-P3
F6
COLO 858 Vemurafenib Trametinib 0.316 0.600
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 447.45 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 164.60 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 948.70
Colo858-P3
F5
WM-115 Vemurafenib Trametinib 0.000 0.600
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 447.75 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 133.22 NGFR rabbit IgG(H+L) / donkey NGFR 2593.32
WM115-P1
E5
WM-115 Vemurafenib PF562271 1.000 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 447.94 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 119.74 NGFR rabbit IgG(H+L) / donkey NGFR 2263.76
WM115-P1
G8
A-375 Vemurafenib Dasatinib 1.000 3.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 448.26 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 160.44 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 208.39
A375-P4
G11
A-375 Vemurafenib PF562271 1.000 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 448.60 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 136.13 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 635.30
A375-P2
G8
WM-115 Vemurafenib Trametinib 0.316 0.600
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 448.75 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 106.75 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 746.32
WM115-P2
F5
COLO 858 Vemurafenib AZD8055 1.000 0.200
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 449.08 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 183.61 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 748.66
Colo858-P9
G6
WM-115 Vemurafenib Defactinib 0.000 3.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 449.61 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 155.85 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 3343.29
WM115-P2
E7
A-375 Vemurafenib Dasatinib 0.316 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 449.80 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 172.05 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 327.81
A375-P3
C11
A-375 Vemurafenib Dasatinib 1.000 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 451.00 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 158.28 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 691.02
A375-P2
G10
WM-115 Vemurafenib Dasatinib 1.000 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 451.42 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 127.73 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 288.35
WM115-P3
D10
WM-115 Vemurafenib PF562271 0.316 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 451.63 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 105.14 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 424.95
WM115-P3
F8
WM-115 Vemurafenib Saracatinib 1.000 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 451.91 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 92.09 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 172.48
WM115-P10
D2
COLO 858 Vemurafenib PF562271 1.000 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 451.99 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 128.69 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 434.70
Colo858-P2
D9
WM-115 Vemurafenib Trametinib 0.316 0.200
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 452.41 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 133.24 NGFR rabbit IgG(H+L) / donkey NGFR 3213.79
WM115-P1
C4
COLO 858 Vemurafenib JNK-IN-8 1.000 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 452.54 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 159.43 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 240.59
Colo858-P8
D9
A-375 Vemurafenib JNK-IN-8 1.000 3.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 452.80 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 127.71 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 425.14
A375-P8
G9
WM-115 Vemurafenib 0.316
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 453.28 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 118.64 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 249.06
WM115-P4
C3
WM-115 Vemurafenib Trametinib 0.316 0.600
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 453.51 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 135.82 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 731.90
WM115-P2
C5