Phosphorylation state and protein levels measured by imaging in BRAF(V600E/D) melanoma cell lines following treatment with combinations of two compounds (immunofluorescence dataset 1 of 4) - Dataset (ID:20274)

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Cell Small Molecule 1 Small Molecule 2 Small Mol 1 Concentration Small Mol 2 Concentration Small Mol Conc Unit Time Point Time Point Unit Replicate Primary Antibody (488) Secondary Antibody (488) Protein Detected (488) Mean Fluorescence Intensity (488) Primary Antibody (568) Secondary Antibody (568) Protein Detected (568) Mean Fluorescence Intensity (568) Primary Antibody (647) Secondary Antibody (647) Protein Detected (647) Mean Fluorescence Intensity (647) Plate Name Plate Well
A-375 Vemurafenib Trametinib 0.000 0.600
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 325.60 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 148.87 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 851.11
A375-P3
B5
COLO 858 Vemurafenib Pictilisib 1.000 3.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 325.98 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 123.65 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 273.97
Colo858-P10
G5
A-375 Vemurafenib JNK-IN-8 1.000 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 326.16 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 102.57 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 297.95
A375-P9
G8
A-375 Vemurafenib PF562271 0.316 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 326.18 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 125.96 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 512.01
A375-P3
C8
COLO 858 Vemurafenib PF562271 0.316 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 326.69 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 138.54 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 734.57
Colo858-P2
C9
COLO 858 Vemurafenib PF562271 0.316 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 326.90 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 155.90 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 250.06
Colo858-P4
C9
COLO 858 Vemurafenib Trametinib 0.000 0.600
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 328.47 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 131.35 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 411.58
Colo858-P4
E5
COLO 858 Vemurafenib Defactinib 0.316 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 328.77 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 155.25 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 545.11
Colo858-P3
C7
COLO 858 Vemurafenib Saracatinib 1.000 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 328.92 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 156.06 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 328.01
Colo858-P10
G2
WM-115 Vemurafenib 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 330.57 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 78.62 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 180.73
WM115-P4
G2
COLO 858 Vemurafenib Defactinib 0.316 3.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 331.57 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 133.03 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 675.62
Colo858-P2
F7
WM-115 Vemurafenib Dasatinib 1.000 3.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 331.92 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 96.47 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 122.36
WM115-P4
G11
COLO 858 Vemurafenib Defactinib 1.000 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 333.65 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 159.51 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 359.15
Colo858-P2
D7
WM-115 Vemurafenib Dasatinib 0.316 3.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 334.98 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 110.25 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 108.33
WM115-P4
F11
WM-115 Vemurafenib Dasatinib 0.316 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 335.14 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 108.46 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 112.45
WM115-P4
F10
COLO 858 Vemurafenib AZD8055 1.000 0.200
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 335.63 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 128.69 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 38.37
Colo858-P8
G6
A-375 Vemurafenib Dasatinib 1.000 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 337.51 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 141.47 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 415.59
A375-P3
D10
WM-115 Vemurafenib PF562271 1.000 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 337.71 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 105.57 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 1142.99
WM115-P2
D8
WM-115 Vemurafenib JNK-IN-8 1.000 3.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 338.21 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 91.80 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 187.51
WM115-P8
G9
WM-115 Vemurafenib Trametinib 0.000 0.200
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 338.40 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 110.02 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 186.62
WM115-P4
B4
WM-115 Vemurafenib Dasatinib 1.000 3.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 338.44 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 101.42 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 334.82
WM115-P2
G11
COLO 858 Vemurafenib Defactinib 1.000 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 338.46 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 172.71 NGFR rabbit IgG(H+L) / donkey NGFR 272.57
Colo858-P1
D6
WM-115 Vemurafenib Trametinib 1.000 0.600
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 338.96 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 115.06 NGFR rabbit IgG(H+L) / donkey NGFR 3192.24
WM115-P1
D5
WM-115 Vemurafenib Palbociclib 0.000 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 339.36 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 109.01 NGFR rabbit IgG(H+L) / donkey NGFR 1247.51
WM115-P7
E10
COLO 858 Vemurafenib Defactinib 1.000 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 340.85 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 136.56 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 534.18
Colo858-P2
G6