Phosphorylation state and protein levels measured by imaging in BRAF(V600E/D) melanoma cell lines following treatment with combinations of two compounds (immunofluorescence dataset 1 of 4) - Dataset (ID:20274)

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Cell Small Molecule 1 Small Molecule 2 Small Mol 1 Concentration Small Mol 2 Concentration Small Mol Conc Unit Time Point Time Point Unit Replicate Primary Antibody (488) Secondary Antibody (488) Protein Detected (488) Mean Fluorescence Intensity (488) Primary Antibody (568) Secondary Antibody (568) Protein Detected (568) Mean Fluorescence Intensity (568) Primary Antibody (647) Secondary Antibody (647) Protein Detected (647) Mean Fluorescence Intensity (647) Plate Name Plate Well
A-375 Vemurafenib Saracatinib 0.316 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 583.64 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 137.10 NGFR rabbit IgG(H+L) / donkey NGFR 286.84
A375-P7
F2
COLO 858 Vemurafenib Pictilisib 0.000 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 2097.61 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 302.09 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 153.69
Colo858-P9
E4
A-375 Vemurafenib Dasatinib 1.000 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 451.00 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 158.28 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 691.02
A375-P2
G10
A-375 Vemurafenib Palbociclib 0.316 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 354.17 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 209.98 NGFR rabbit IgG(H+L) / donkey NGFR 635.87
A375-P7
F10
A-375 Vemurafenib Pictilisib 1.000 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 488.77 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 173.57 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 268.96
A375-P10
D4
COLO 858 Vemurafenib Dasatinib 1.000 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 600.35 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 183.29 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 209.16
Colo858-P3
G10
A-375 Vemurafenib Defactinib 0.000 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 2453.91 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 457.23 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 7382.49
A375-P2
B6
COLO 858 Vemurafenib PF562271 1.000 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 401.40 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 139.60 NGFR rabbit IgG(H+L) / donkey NGFR 422.47
Colo858-P1
D8
A-375 Vemurafenib JNK-IN-8 0.000 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 2413.32 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 408.16 NGFR rabbit IgG(H+L) / donkey NGFR 329.47
A375-P7
E8
COLO 858 Vemurafenib Dasatinib 0.000 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 3740.18 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 484.10 NGFR rabbit IgG(H+L) / donkey NGFR 116.54
Colo858-P1
E10
A-375 Vemurafenib PF562271 0.316 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 491.30 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 152.20 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 1191.87
A375-P2
F8
A-375 Vemurafenib Dasatinib 1.000 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 536.52 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 174.46 NGFR rabbit IgG(H+L) / donkey NGFR 300.18
A375-P1
D10
COLO 858 Vemurafenib Palbociclib 0.316 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 -32.74 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 96.94 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 324.72
Colo858-P10
C10
COLO 858 Vemurafenib PF562271 0.316 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 391.70 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 155.91 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 642.08
Colo858-P3
F8
COLO 858 Vemurafenib Defactinib 1.000 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 459.68 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 183.60 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 594.63
Colo858-P3
G6
A-375 Vemurafenib Pictilisib 0.000 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 2339.94 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 429.64 NGFR rabbit IgG(H+L) / donkey NGFR 360.80
A375-P7
E4
A-375 Vemurafenib Defactinib 0.316 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 522.88 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 155.12 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 1413.46
A375-P2
F6
COLO 858 Vemurafenib Dasatinib 0.316 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 509.69 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 173.36 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 192.18
Colo858-P3
F10
A-375 Vemurafenib Saracatinib 0.000 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 2657.88 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 504.90 NGFR rabbit IgG(H+L) / donkey NGFR 261.89
A375-P7
E2
A-375 Vemurafenib Saracatinib 1.000 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 532.78 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 182.44 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 261.07
A375-P10
D2
A-375 Vemurafenib Palbociclib 0.000 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 1109.41 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 193.91 NGFR rabbit IgG(H+L) / donkey NGFR 477.86
A375-P7
E10
A-375 Vemurafenib JNK-IN-8 1.000 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 345.98 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 120.79 NGFR rabbit IgG(H+L) / donkey NGFR 369.72
A375-P7
D8
A-375 Vemurafenib Dasatinib 0.316 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 550.44 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 170.79 NGFR rabbit IgG(H+L) / donkey NGFR 155.36
A375-P1
F10
COLO 858 Vemurafenib PF562271 0.000 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 2416.35 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 295.01 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 178.42
Colo858-P3
E8
COLO 858 Vemurafenib Dasatinib 0.000 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 3971.65 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 473.49 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 136.32
Colo858-P3
E10