Phosphorylation state and protein levels measured by imaging in BRAF(V600E/D) melanoma cell lines following treatment with combinations of two compounds (immunofluorescence dataset 1 of 4) - Dataset (ID:20274)

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Cell Small Molecule 1 Small Molecule 2 Small Mol 1 Concentration Small Mol 2 Concentration Small Mol Conc Unit Time Point Time Point Unit Replicate Primary Antibody (488) Secondary Antibody (488) Protein Detected (488) Mean Fluorescence Intensity (488) Primary Antibody (568) Secondary Antibody (568) Protein Detected (568) Mean Fluorescence Intensity (568) Primary Antibody (647) Secondary Antibody (647) Protein Detected (647) Mean Fluorescence Intensity (647) Plate Name Plate Well
COLO 858 Vemurafenib Trametinib 1.000 0.600
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 415.48 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 142.44 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 287.16
Colo858-P2
D5
COLO 858 Vemurafenib Trametinib 0.000 0.600
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 402.57 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 143.40 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 885.01
Colo858-P3
B5
COLO 858 Vemurafenib AZD8055 1.000 0.600
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 72.49 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 109.75 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 309.34
Colo858-P10
D7
COLO 858 Vemurafenib AZD8055 0.000 0.600
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 724.98 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 167.96 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 91.85
Colo858-P10
E7
COLO 858 Vemurafenib AZD8055 0.316 0.600
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 163.55 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 117.33 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 270.97
Colo858-P10
C7
A-375 Vemurafenib AZD8055 0.000 0.600
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 1446.04 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 307.33 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 471.65
A375-P8
B7
A-375 Vemurafenib Trametinib 0.316 0.600
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 463.41 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 158.22 NGFR rabbit IgG(H+L) / donkey NGFR 1124.92
A375-P1
C5
A-375 Vemurafenib Trametinib 0.316 0.600
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 182.46 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 119.76 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 395.54
A375-P4
C5
A-375 Vemurafenib AZD8055 0.316 0.600
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 989.80 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 212.12 NGFR rabbit IgG(H+L) / donkey NGFR 424.21
A375-P7
F7
A-375 Vemurafenib Trametinib 0.316 0.600
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 282.51 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 133.75 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 401.92
A375-P4
F5
A-375 Vemurafenib AZD8055 0.000 0.600
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 1304.14 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 256.23 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 279.80
A375-P9
E7
COLO 858 Vemurafenib AZD8055 0.316 0.600
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 239.09 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 130.90 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 262.54
Colo858-P10
F7
COLO 858 Vemurafenib Trametinib 0.316 0.600
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 360.75 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 126.77 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 402.98
Colo858-P4
C5
A-375 Vemurafenib Defactinib 0.000 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 2453.91 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 457.23 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 7382.49
A375-P2
B6
COLO 858 Vemurafenib PF562271 1.000 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 529.15 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 153.33 NGFR rabbit IgG(H+L) / donkey NGFR 402.57
Colo858-P1
G8
A-375 Vemurafenib Saracatinib 0.000 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 2084.99 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 403.10 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 190.22
A375-P10
B2
A-375 Vemurafenib Saracatinib 0.316 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 583.64 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 137.10 NGFR rabbit IgG(H+L) / donkey NGFR 286.84
A375-P7
F2
COLO 858 Vemurafenib Palbociclib 0.000 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 1145.34 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 202.17 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 96.67
Colo858-P10
B10
A-375 Vemurafenib Dasatinib 0.316 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 348.98 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 146.33 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 218.98
A375-P4
C10
A-375 Vemurafenib PF562271 0.000 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 1984.98 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 378.90 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 182.56
A375-P4
B8
A-375 Vemurafenib Dasatinib 0.000 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 2728.23 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 495.24 NGFR rabbit IgG(H+L) / donkey NGFR 132.92
A375-P1
E10
COLO 858 Vemurafenib Defactinib 1.000 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 627.55 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 151.58 NGFR rabbit IgG(H+L) / donkey NGFR 225.14
Colo858-P1
G6
COLO 858 Vemurafenib Saracatinib 0.000 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 2259.14 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 363.05 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 145.26
Colo858-P10
B2
A-375 Vemurafenib Defactinib 0.000 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 2059.99 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 371.47 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 198.06
A375-P4
B6
COLO 858 Vemurafenib Defactinib 0.316 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 717.48 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 197.50 NGFR rabbit IgG(H+L) / donkey NGFR 163.65
Colo858-P1
F6